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Yu J.,Zhejiang University | Yu J.,Key Laboratory of Combined Multiorgan Transplantation | Fei J.,Zhejiang University | Fei J.,Key Laboratory of Combined Multiorgan Transplantation | And 5 more authors.
Journal of International Medical Research | Year: 2012

Objectives: To investigate the myocardially protective effects of Salvia miltiorrhiza injection in streptozotocininduced diabetic rats and the possible mechanisms involved. METHODS: Adult male Sprague-Dawley rats were randomized into three groups (n = 10 per group): diabetes, no treatment (Sm-); diabetes, S. miltiorrhiza injection (Sm+); control (no diabetes; saline treatment). After model induction and 4 weeks' treatment, heart function of five rats from each group was tested by Langendorff isolated in vivo heart perfusion. In the remaining rats, pathological changes of the myocardium were observed by haematoxylin and eosin staining, and protein levels of thrombospondin-1 (TSP-1) and transforming growth factor-β1 (TGFβ1) were assessed by immuno histo - chemistry. RESULTS: Left ventricular systolic end pressure and left ventricular developed pressure were significantly improved in the Sm+ group compared with the Sm- group. Pathological changes were ameliorated through significantly reduced TSP-1 and TGF-β1 protein levels. CONCLUSIONS: S. miltiorrhiza injection may improve the heart function of diabetic rats and protect against cardiomyopathy by downregulating TSP-1 and TGF-β1 in myocardial tissue. © 2012 Field House Publishing LLP.

Yu Z.,Zhejiang University | Yu Z.,Key Laboratory of Combined Multiorgan Transplantation | Zhou X.,Zhejiang University | Zhou X.,Key Laboratory of Combined Multiorgan Transplantation | And 6 more authors.
Molecular Medicine Reports | Year: 2015

The aim of this study was to investigate the effect of cyclosporine A (CsA) and tacrolimus (FK506) on interleukin.15 (IL.15) production during acute rejection following heart transplantation in mice. Inbred male Balb/c (H.2d) and C57BL/6 (H.2b) mice were used to establish a heterotopic intra.abdominal cardiac transplantation model. The mice were divided in four groups: syngeneic control, allogeneic acute rejection, allogeneic rejection treated with CsA, and allogeneic rejection treated with FK506. The expression of IL.15, IL.2, and tumor necrosis factor-α (TNF-α) was measured using reverse transcription.polymerase chain reaction (RT.PCR) and western blotting. A low level of IL.15 was detected in transplanted hearts of the control group, with a significant increase observed in the allogeneic acute rejection group. Compared to the allogeneic acute rejection group, IL.15 expression was significantly decreased in the CsA.and FK506.treated allogeneic rejection groups. The TNF-α expression pattern was similar to that of IL.15 in all groups. IL.2 expression was increased in the allogeneic acute rejection group and was inhibited in mice treated with CsA and FK506. In conclusion, increased IL.15 expression in rejected murine heart grafts may be reduced by CsA and FK506 in vivo.

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