Time filter

Source Type

Feng Z.-X.,Affiliated Hospital of Zunyi Medical College | Chen Q.,Affiliated Hospital of Zunyi Medical College | Xiao J.-H.,Key Laboratory of Cell Engineering of Guizhou Province | Feng J.,Affiliated Hospital of Zunyi Medical College
Chinese Journal of Cancer Prevention and Treatment | Year: 2016

OBJECTIVE: Survivin and IL-8 were highly expressed in acute myeloid leukemia and a variety of malignant tumors, and closely related to the therapeutic effect and prognosis of leukemia. The objective of this study was investigate the effect of sCD40L on proliferation of HL-60 cells and expressions of IL-8 and Survivin, and explore its possible mechanism. METHODS: HL-60 cells were cultered with three different concentrations of sCD40L (2, 4 and 6 μg/mL) for 24, 48 and 72 h, respectively. The MTT assay was used to examine the effect of different concentrations of sCD40L on Leukemia HL-60 cell proliferation in vitro at different incubation times. RT-PCR was used to determine the effect of different concentrations of sCD40L on the expression of Survivin mRNA in HL-60 cells at different incubation times. ELISA was used to detect changes in cytokine IL-8 levels in the culture supernatants of leukemia HL-60 cell of different concentrations of sCD40L at different incubation times. RESULTS: Three different concentrations of sCD40L could all significantly inhibit HL-60 cell proliferation and there was a dose-dependent tendency. There was statistical significance between different concentrations (F=150.076, P<0.05), and there was statistical significance between different time (F=358.969, P<0.05). sCD40L had the best inhibitory effect on HL-60 for 48 h. Three different concentrations of sCD40L could all significantly inhibit the expression of Survivin mRNA in HL-60 cells. The 6.00 μg/mL sCD40L group was the lowest. In the range of 24-72 h, Survivin mRNA expression in HL-60 cells was the lowest for 48 h. The expression rates of Survivin mRNA was respectively 89.7±6.2. Compared with As2O3 control group, it was statistically significant (P<0.05). Three different concentrations of sCD40L could all significantly inhibit IL-8 secretion in HL-60 cells. The 6.00 μg/mL group had the lowest expression rate. In the range of 24-72 h, IL-8 secretion in HL60 cells was the lowest for 48 h. The expression rates of IL-8 was respectively 380.2±29.5. Compared with As2O3 control group, it was statistically significant (P<0.05). CONCLUSIONS: sCD40L can significantly inhibit the proliferation of HL-60 cells in vitro, yet it is concentration and time dependent. The mechanisms of sCD40L in inhibiting the proliferation of HL-60 cells and inducing apoptosis in HL-60 cells may be related to their inhibition of IL-8 and Survivin expression. © 2016, Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.

Loading Key Laboratory of Cell Engineering of Guizhou Province collaborators
Loading Key Laboratory of Cell Engineering of Guizhou Province collaborators