Time filter

Source Type

Yan C.,Chinese Academy of Sciences | Yan C.,Tianjin Medical University | Yan C.,Key Laboratory of Cancer Immunology and Biotherapy | Yang M.,Chinese Academy of Sciences | And 7 more authors.

Mesenchymal stem cells (MSCs) are promising vehicles for delivering therapeutic agents in tumor therapy. Human umbilical cord-derived mesenchymal stem cells (HUMSCs) resemble bone marrow-derived MSCs with respect to hepatic differentiation potential in injured livers in animals, while their hepatic differentiation under the hepatocarcinoma microenvironment is unclear. In this study, HUMSCs were isolated and transduced by lentiviral vectors coding the soluble human tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL) gene driven by alpha-fetoprotein (AFP) promoter to investigate the therapeutic effects of these HUMSC against orthotopically implanted hepatocarcinoma in mice. We showed that HUMSCs can be transduced by lentivirus efficiently. HUMSCs developed cuboidal morphology, and expressed AFP and albumin in a two-step protocol. HUMSCs were capable of migrating to hepatocarcinoma in vitro as well as in vivo. In the orthotopical hepatocarcinoma microenvironment, the AFP promoter was activated during the early hepatic differentiation of HUMSCs. After intravenous injected, MSC.AFPILZ-sTRAIL expressed sTRAIL exclusively at the tumor site, and exhibited significant antitumor activity. This effect was stronger when in combination with 5-FU. The treatment was tolerated well in mice. Collectively, our results provide a potential strategy for targeted tumor therapy relying on the use of the tumor tropism and specific differentiation of HUMSCs as vehicles. © 2013 Elsevier Ltd. Source

Yang L.,Tianjin Medical University | Yang L.,Key Laboratory of Cancer Immunology and Biotherapy | Eksioglu E.A.,H. Lee Moffitt Cancer Center and Research Institute | Wei S.,H. Lee Moffitt Cancer Center and Research Institute

Myelodysplastic syndromes (MDSs) are hematopoietic stem cell disorders with a high potential to develop into acute myeloid leukemia (AML). We have recently demonstrated that naïve T cells, but not memory T cells, from MDS patients exhibit a pronounced deficiency in the mRNA coding for the catalytic subunit of telomerase (hTER T). We discuss the importance of this finding for lymphocytic homeostasis in MDS patients. © 2013 Landes Bioscience. Source

Dong W.,Tianjin Hongqiao Hospital | Qian Y.,Tianjin Medical University | Qian Y.,Key Laboratory of Cancer Immunology and Biotherapy | Yang L.,Tianjin Medical University | Yang L.,Key Laboratory of Cancer Immunology and Biotherapy
Leukemia Research

Telomeres are specialized structures maintaining chromosome integrity during cellular division and preventing from premature senescence and apoptosis. The rate-limiting component of telomerase is human telomerase reverse transcriptase (hTERT), for which multiple transcripts exist. The aim of this work was to characterize hTERT splice variants in MDS and its relation to telomerase activity, telomere length and hTERT expression. The telomere length in PBMCs of patients with MDS cases was significantly shorter compared to controls (n= 30, p= 0.002). MDS patients had significantly higher basal telomerase activity (p= 0.022) and higher total hTERT (p= 0.007), α+β+ hTERT variant (p= 0.016) and α+β- hTERT variant expression than control. The ratio of α+β- transcript to α+β+ transcript was significantly increased in cases (p= 0.039). This study provided a detailed insight into the hTERT transcript pattern in MDS while correlation analysis showed that only telomerase activity was significantly correlated with total hTERT expression in MDS. © 2014 Elsevier Ltd. Source

Wang F.,Tianjin Medical University | Wang F.,Key Laboratory of Cancer Immunology and Biotherapy | Ren X.,Tianjin Medical University | Ren X.,Key Laboratory of Cancer Immunology and Biotherapy | And 2 more authors.
Oncology Letters

MicroRNAs (miRNAs) are a family of small endogenous noncoding RNAs and their altered expression has been associated with various cellular functions, including cell development, proliferation, differentiation, apoptosis, signal transduction, tumorigenesis and cancer progression. Accumulating evidence has indicated that miRNA (miR)-150 plays an essential regulatory role in normal hematopoiesis and tumorigenesis; therefore, miR-150 may be a potential biomarker and therapeutic target in the diagnosis and treatment of various malignancies. The aim of the present review was to summarize the current knowledge on the functions and regulatory mechanism of miR-150 as an oncogene or tumor suppressor gene in solid tumors. In addition, its potential application as a tumor biomarker, targeted therapeutic strategy and index of prognosis in various cancer types was investigated. © 2015, Spandidos Publications. All rights reserved. Source

Sang Y.,Tianjin Medical University | Sang Y.,Key Laboratory of Cancer Immunology and Biotherapy | Yan F.,Tianjin Medical University | Yan F.,Key Laboratory of Cancer Immunology and Biotherapy | And 2 more authors.

CRLs (Cullin-RING E3 ubiquitin ligases) are the largest E3 ligase family in eukaryotes, which ubiquitinate a wide range of substrates involved in cell cycle regulation, signal transduction, transcriptional regulation, DNA damage response, genomic integrity, tumor suppression and embryonic development. CRL4 E3 ubiquitin ligase, as one member of CRLs family, consists of a RING finger domain protein, cullin4 (CUL4) scaffold protein and DDB1-CUL4 associated substrate receptors. The CUL4 subfamily includes two members, CUL4A and CUL4B, which share extensively sequence identity and functional redundancy. Aberrant expression of CUL4 has been found in a majority of tumors. Given the significance of CUL4 in cancer, understanding its detailed aspects of pathogenesis of human malignancy would have significant value for the treatment of cancer. Here, the work provides an overview to address the role of CRL4 E3 ubiquitin ligase in cancer development and progression, and discuss the possible mechanisms of CRL4 ligase involving in many cellular processes associated with tumor. Finally, we discuss its potential value in cancer therapy. Source

Discover hidden collaborations