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Ding T.,Key Laboratory of Breast Cancer Prevention and Therapy | Gu F.,Key Laboratory of Breast Cancer Prevention and Therapy | Fu L.,Key Laboratory of Breast Cancer Prevention and Therapy | Ma Y.-J.,Tianjin Medical University
Journal of Clinical Neuroscience | Year: 2010

Aquaporin-4 (AQP4) is the most important member of the aquaporin (AQP) family in the central nervous system and has a key role in maintaining water and ion homeostasis. It is clear that AQP4 participates in brain edema after injury and other brain diseases. Various studies report that AQP4 is significantly up-regulated in glioblastoma multiforme compared to low-grade glioma and normal brain tissue, suggesting that it might be involved in tumor malignancy as well as regulation of brain edema. Recent discoveries of AQP4 involvement in cell migration and cytoskeleton organization suggest that AQP4 has a critical role in glioma malignancy. This review focuses on the newly discovered functions and molecular mechanisms of AQP4 in infiltrative gliomas. © 2010 Elsevier Ltd. All rights reserved. Source

Liu B.,Key Laboratory of Breast Cancer Prevention and Therapy | Liu B.,Tianjin Medical University | Qu J.,Key Laboratory of Breast Cancer Prevention and Therapy | Qu J.,Tianjin Medical University | And 10 more authors.
Oncotarget | Year: 2015

MiR-195 suppresses tumor growth and is associated with better survival outcomes in several malignancies including non-small cell lung cancer (NSCLC). Our previous study showed high miR-195 plasma levels associated with favorable overall survival of non-smoking women with lung adenocarcinoma. To further elucidate role of miR-195 in NSCLC, we conducted in vitro experiment as well as clinical studies in a cohort of 299 NSCLC samples. We demonstrated that miR-195 expression was lower in tumor tissues and was associated with poor survival outcome. Overexpression of miR-195 suppressed tumor cell growth, migration and invasion. We discovered that CHEK1 was a direct target of miR-195, which decreased CHEK1 expression in lung cancer cells. High expression of CHEK1 in lung tumors was associated with poor overall survival. Our results suggest that miR-195 suppresses NSCLC and predicts lung cancer prognosis. Source

Li Y.,Tianjin Medical University | Wu J.,Tianjin Medical University | Zhang W.,Tianjin Medical University | Zhang N.,Tianjin Medical University | And 3 more authors.
British Journal of Cancer | Year: 2013

Background:Early serum detection is of critical importance to improve the therapy for hepatocellular carcinoma (HCC), one of the most deadly cancers. Hepatitis infection is a leading cause of HCC.Methods:In the present study, we collected total serum samples with informed consent from 80 HCC patients with HBV (+)/cirrhosis (+), 80 patients with benign diseases (50 liver cirrhosis patients and 30 HBV-infected patients) and 60 healthy controls. Analysis was by using surface-enhanced laser desorption/ionisation-time-of-flight mass spectroscopy (SELDI-TOF-MS) to find new serum markers of HCC. SELDI peaks were isolated by SDS-PAGE, identified by LC-MS/MS and validated by immunohistochemistry (IHC) in liver tissues. Migration and invasion assay were performed to test the ability of cell migration and invasion in vitro.Results:SELDI-TOF-MS revealed a band at 7777 M/Z in the serum samples from HCC patients but not from healthy controls or patients with benign diseases. The protein (7777.27 M/Z) in the proteomic signature was identified as C-C motif chemokine 15 (CCL15) by peptide mass fingerprinting. A significant increase in serum CCL15 was detected in HCC patients. Functional analysis showed that HCC cell expressed CCL15, which in turn promoted HCC cell migration and invasion.Conclusion:CCL15 may be a specific proteomic biomarker of HCC, which has an important role in tumorigenesis and tumour invasion. © 2013 Cancer Research UK. All rights reserved. Source

Ma T.,Tianjin Medical University | Ma T.,Key Laboratory of Breast Cancer Prevention and Therapy | Ma T.,Key Laboratory of Cancer Prevention and Therapy | Yang L.,Tianjin Medical University | And 5 more authors.
Oncology Reports | Year: 2015

Trastuzumab (Herceptin) has been widely used in breast cancer treatment. However, the majority of cancers that initially respond to trastuzumab begin to progress again within 1 year. Despite the high resistance rate, the molecular mechanisms underlying this desease are not well understood. In the present study, microRNA (miRNA-542-3p modulated trastuzumab resistance in SKBR3 and MCF7/Her2 breast cancer cell lines. Trastuzumab induced miRNA-542-3p expression in SKBR3 and MCF7/Her2 cells. Furthermore, knockdown of miRNA-542-3p in the two cell lines resulted in decreased drug sensitivity to trastuzumab and cell apoptosis. The blockage of G1/S checkpoint by trastuzumab was rescued as well-miRNA-542-3p knockdown also activated the phosphatidylinositol 3-kinase (PI3K)-Akt pathway, while LY294002 reversed the effect of miRNA-542-3p knockdown. In summary, the results suggested that miRNA-542-3p downregulation may contribute to the trastuzumab resistance in breast cancer via, at least in part, the PI3K-akt pathway. Our findings provide new molecular mechanisms in trastuzumab resistance. Source

Liu B.,Key Laboratory of Breast Cancer Prevention and Therapy | Liu B.,Tianjin Medical University | Zhang X.,Key Laboratory of Breast Cancer Prevention and Therapy | Zhang X.,Tianjin Medical University | And 16 more authors.
Oncotarget | Year: 2016

A single-nucleotide polymorphism (SNP) locus rs16917496 (T > C) within the 3'-untranslated region (3'-UTR) of SET8 was associated with susceptibility in several malignancies including breast cancer. To further elucidate the prognostic relevance of this SNP in breast cancer, we conducted a clinical study as well as SET8 expression analysis in a cohort of 1,190 breast cancer patients. We demonstrated the expression levels of SET8 in TT genotype were higher than in CC genotypes, and high levels of SET8 were associated with poor survival. SET8 expression was significantly higher in breast tumor tissue than in paired adjacent normal tissue. In addition, survival analysis in 315 patients showed SNP rs16917496 was an independent prognostic factor of breast cancer outcome with TT genotype associated with poor survival compared with CC/CT genotypes. Thus, this SNP may serve as a genetic prognostic factor and a treatment target for breast cancer. Future studies are warranted. Source

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