Zhang M.,Tianjin Medical University |
Zhou Y.,Tianjin Medical University |
Hu Y.,Tianjin Medical University |
Zhang J.,Tianjin Medical University |
Zhang J.,Key Laboratory of Breast Cancer Prevention
National Medical Journal of China | Year: 2015
Objective To investigatethe effect of NVP-BKM120 on the triple-negative breast cancer cell lines. Methods Breast cancer cell line MDA-MB-231 and Cal51 were divided into control group and experimental group. The inhibitory effects of BKM120 were evaluated by MTT assays. The drug effects on CSC population and characteristics were investigated through mammosphere formation assay and colony formation assay. Western blot was used to observe the expressionof related protein. The BALB/c mice were injected with stem cells (SCs) and different treatments were administered subsequently. Results In MDA-MB-231 cell lines, IC50 of BKM120 was (20.01 ± 3. 46) jjimol/L for SCs and(3.07±0. 14) jimol/L for total cells. BKM120 significantly inhibit the cell growth,in vitrocloning and microspheres formed of triple-negative breast cancer cells. BKM120 can inhibit tumor growth in nude mice without significant adverse reactions. Conclusion BKM120 can significantly inhibit the proliferation of the triple-negative breast cancer cell lines.
Li C.-Y.,Tianjin Medical University |
Li C.-Y.,Key Laboratory of Breast Cancer Prevention |
Li C.-Y.,Key Laboratory of Cancer Prevention and Therapy in Tianjin |
Zhang S.,Tianjin Medical University |
And 14 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2013
Aims: To determine the clinical, pathological and prognostic features associated with triple-negative breast cancer (TNBC). Methods: Clinical and histologic data of 21,749 breast cancer patients who were treated at Tianjin Medical University Cancer Institute andHospital between July 2002 and December 2011 were collected. Patients were divided into two groups: those with TNBC and those with other types of breast cancer. Patients and tumor characteristics were compared between the two groups using the Chi-square test. The prognostic results of 9,823 patients in the study population were also analyzed to determinelong-term survival rates in the two groups of breast cancer patients. Results: Among the breast cancer patients treated in our hospital between 2003 and 2011, 10.4%-13.5% of themhad triple-negative breast cancers. Data analyses revealed significant differences in disease onset age, family history of breast cancer, tumor size, tumor histologic grade, lymph note positivity and metastatic status between TNBC and non-TNBC patients. There were also significant differences in 5-year, 7-year and 9-year disease-free and 7-year and 9-year overall survival probability between the groups. Conclusions:TNBC are associated with younger disease onset age, larger tumor size, higher rate of axillary lymph node positivity, and higher tumor histologic grade. TNBC is also related to family history of breast cancer, increased metastatic risk and poor prognosis.