Key Laboratory of Biosynthesis of Natural Products

Qingquan, China

Key Laboratory of Biosynthesis of Natural Products

Qingquan, China
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Zhao R.-Y.,Chinese Academy of Sciences | Zhao R.-Y.,Key Laboratory of Biosynthesis of Natural Products | Zhao R.-Y.,Key Laboratory of Bioactive Substances and Resources Utilization | Xiao W.,Chinese Academy of Sciences | And 11 more authors.
Journal of Industrial Microbiology and Biotechnology | Year: 2010

The gene encoding squalene synthase (GfSQS) was cloned from Fusarium fujikuroi (Gibberella fujikuroi MP-C) and characterized. The cloned genomic DNA is 3,267 bp in length, including the 5'-untranslated region (UTR), 3'-UTR, four exons, and three introns. A noncanonical splice-site (CA-GG, or GC-AG) was found at the first intron. The open reading frame of the gene is 1,389 bp in length, corresponding to a predicted polypeptide of 462 amino acid residues with a MW 53.4 kDa. The predicted GfSQS shares at least four conserved regions involved in the enzymatic activity with the SQSs of varied species. The recombinant protein was expressed in E. coli and detected by SDS-PAGE and western blot. GC-MS analysis showed that the wild-type GfSQS could catalyze the reaction from farnesyl diphosphate (FPP) to squalene, while the mutant mGfSQS (D82G) lost total activity, supporting the prediction that the aspartate-rich motif (DTXED) in the region I of SQS is essential for binding of the diphosphate substrate. © 2010 Society for Industrial Microbiology.

Lv L.,Peking Union Medical College | Lv L.,Key Laboratory of Biosynthesis of Natural Products | Lv L.,Chinese Academy of Sciences | Tian X.-Y.,Peking Union Medical College | And 3 more authors.
Journal of Asian Natural Products Research | Year: 2010

Three new C-glucosylanthrones, 3′-O-acetyl-5-hydroxylaloin A (2), 2′,6′-O-diacetyl-5-hydroxylaloin A (4), and 4′,6′-O- diacetyl-5-hydroxylaloin A (5), along with three known compounds, 5-hydroxyaloin A (1), 6′-acetyl-5-hydroxylaloin A (3), and 4-methoxy-6-(2′, 4′-dihydroxy-6′-methylphenyl)-pyran-2-one (6), were isolated from the leaves of Aloe nobilis, and their structures were elucidated on the basis of spectroscopic evidences. Compounds 1, 2, 4 and 5 showed antioxidant activity with inhibitory rates of 31.0, 34.0, 34.0, and 42.0%, respectively, at 10 -5M. © 2010 Taylor & Francis.

Guo N.,Peking Union Medical College | Guo N.,Key Laboratory of Biosynthesis of Natural Products | Guo N.,Chinese Academy of Sciences | Zhao Y.,Peking Union Medical College | And 5 more authors.
Planta Medica | Year: 2010

3-Oxo-oleanolic acid (1) was biotransformed in growing cultures of the fungus Absidia glauca, resulting in three novel hydroxylated metabolites, identified as 1β-hydroxy-3-oxo-olean-11-en-28,13-lactone (2), 1β,11-dihydroxy-3-oxo-olean-12-en-28-oic acid (3), and 1β,11α, 21β-trihydroxy-3-oxo-olean-12-en-28-oic acid (4). © Georg Thieme Verlag KG Stuttgart · New York.

Niu Y.-J.,Chinese Academy of Sciences | Tao R.-Y.,Chinese Academy of Sciences | Liu Q.,Chinese Academy of Sciences | Tian J.-Y.,Chinese Academy of Sciences | And 4 more authors.
American Journal of Chinese Medicine | Year: 2010

This study explores the effects of 3′-deoxyadenosine, a compound from Cordyceps militaris, on lipid metabolic disorder induced by a high-fat-diet in C57BL/6 mice. These mice had an obese body, lipid metabolic disorder and insulin resistance and were treated orally with 100 mg/kg/day 3′-deoxyadenosine (DA), 15 mg/kg/day rosiglitazone and 150 mg/kg/day fenofibrate, respectively. Compared to the model mice, the body weight gain in DA-treated mice were decreased by 66.5%, serum triglyceride and total cholesterol levels were decreased by 20.7% and 16.7%, respectively, and the triglyceride content in the skeletal muscle was reduced by 41.2%. This treatment also had a significant effect on insulin resistance. In DA-treated mice, the serum insulin levels and homeostasis model assessment of the insulin resistance index were decreased by 30% and 46%, respectively, and the areas under the glucose-time curve were depressed by 18% in the insulin tolerance test and by 21.5% in the oral glucose tolerance test. Finally, the value of glucose infusion rates and insulin induced-glucose uptake into the skeletal muscle in the hyperinsulinemic- euglycemic clamp test were increased by 18% and 41%, respectively, compared to those in the model mice. This data suggests that the effects of DA on lipid metabolic disorder induced by a high-fat-diet may be linked to its improvement on insulin resistance, especially concerning the increase of insulin sensitivity in the skeletal muscle. © 2010 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.

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