Key Laboratory of Biopesticide and Chemical Biology

Fuzhou, China

Key Laboratory of Biopesticide and Chemical Biology

Fuzhou, China
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He H.,Key Laboratory of Biopesticide and Chemical Biology | Wang H.,Fujian Agriculture and forestry University | Lin Z.,Fujian Agriculture and forestry University | Chen R.,Fujian Agriculture and forestry University | And 3 more authors.
Allelopathy Journal | Year: 2011

To further explore the practical application of allelochemicals in rice production, the mixture of five oxygenic terpenoids [(-) carveol, (+) carvone, (-) menthone, (-) carvyl acetate, and (+) cedrol] identified in previous study, was evaluated in green house for their herbicidal activity on paddy weeds. This terpenoid mixture effectively reduced the growth (aboveground dry weight) of most paddy weeds by 90.0% in 2007 and 83.3% in 2009, respectively, over control. However, the growth of two tested hybrid rice cultivars (Shan-You 63 and Te-You-Hang II) was enhanced after the terpenoid mixture treatment. This study suggested that allelochemicals (including terpenoids), could be used as novel component of integrated weed management in rice production.


Lin Y.,Fujian Agriculture and forestry University | Lin Y.,Key Laboratory of Biopesticide and Chemical Biology | Lin Y.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Hussain M.,Fujian Agriculture and forestry University | And 9 more authors.
PLoS ONE | Year: 2016

Herbivore-induced plant volatiles (HIPVs) are clues that help predatory insects search for food. The hypothesis that entomopathogenic fungi, which protect plants, benefit from the release of HIPVs was tested. The plant Arabidopsis thaliana was used as the source of HIPVs. The insect herbivore Lipaphis erysimi (Kaltenbach) was used as the inducer, and the fungal pathogen of the aphid Lecanicillium lecanii was exposed to HIPVs to test our hypothesis. When exposed to aphid-induced A. thaliana volatiles, the mortality of aphids pre-treated with a conidial suspension of L. lecanii, the conidial germination and the appressorial formation were significantly increased compared with the control. The decan-3-ol and 4-methylpentyl isothiocyanate that were detected in the headspace seemed to have positive and negative affection, respectively. Moreover, HIPVs generated from groups of eight aphids per plant promoted significantly increased conidial germination and appressorial formation compared with HIPVs from groups of one, two and four aphids per plant. Our results demonstrated that the pathogenicity of the entomopathogenic fungus L. lecanii was enhanced when exposed to HIPVs and that the HIPVs were affected by the number of insect herbivores that induced them. © 2016 Lin et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Fang C.,Fujian Agriculture and forestry University | Fang C.,Key Laboratory of Biopesticide and Chemical Biology | Yu Y.,Fujian Agriculture and forestry University | Chen W.,Fujian Agriculture and forestry University | And 5 more authors.
Plant Growth Regulation | Year: 2015

Jasmonic acid (JA) and methyl jasmonate are important endogenous signal molecules that can induce plant resistance to biotic and abiotic stresses. Allene oxide cyclase (AOC) is a key enzyme in the biosynthesis of jasmonates. Here, in order to evaluate the correlation between AOC transcript level and the allelopathic potential of rice, gene expression of AOC was genetic operated in allelopathic rice PI312777 and non-allelopathic rice Lemont, using RNA interference (RNAi) and overexpression (OX) techniques respectively. An increase in endogenous JA was found in AOC-OX rice, and the transcript levels of phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, hydroxylase, CoA-ligases, O-methyltransferases, and cinnamoyl alcohol dehydrogenases, which are involved in phenolic acid synthesis, were also up-regulated in AOC-OX transgenic rice when compared with those of the wild type. Higher accumulation of protocatechuic acid, p-hydroxy benzoic acid, ferulic acid, benzoic acid, 4-hydroxy-3-methoxybenzoicacid, and cinnamic acid content were found in the AOC-OX transgenic line. The increase of phenolic acids in the AOC-OX transgenic lines led to enhanced inhibitory rates on barnyardgrass, whereas the reverse was true in the AOC-RNAi transgenic line. The results suggest that AOC can regulate the synthesis of rice phenolic acids and thus allelopathic inhibition on barnyardgrass. © 2015 Springer Science+Business Media Dordrecht


Hussain M.,Fujian Agriculture and forestry University | Hussain M.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Hussain M.,Key Laboratory of Biopesticide and Chemical Biology | Lin Y.,Fujian Agriculture and forestry University | And 4 more authors.
Journal of Thermal Analysis and Calorimetry | Year: 2016

The longevity of Asian citrus psyllid, Diaphorina citri, infected by an entomopathogenic fungus, Isaria fumosorosea, was studied by microcalorimetry. Experimental results of microcalorimetry suggest that in the process of metabolism of D. citri heat or heat flow declined exponentially. The metabolism of D. citri inhibited by increasing temperature, and the longevity of D. citri decreased gradually with respect to temperature. The longevity of D. citri almost reaches 500 h at 25 °C and D. citri died within 130 h at 41 °C under high-temperature exposure. The same phenomenon, decrease in longevity and metabolism inhibition, was observed under the low-temperature exposure. The supercooling point of insects can be derived from a cooling curve plotting the temperature versus time by using microcalorimetry. The heat released due to phase change can be determined by using microcalorimeter. Our results indicated that microcalorimetry can be used to measure the metabolism of herbivore insects. © 2016 Akadémiai Kiadó, Budapest, Hungary


Wang H.B.,Fujian Agriculture and forestry University | He H.B.,Key Laboratory of Biopesticide and Chemical Biology | He H.B.,Fujian Agriculture and forestry University | Yang G.D.,Fujian Agriculture and forestry University | And 4 more authors.
Acta Physiologiae Plantarum | Year: 2010

This paper reports on a hydroponics experiment that was conducted to investigate the effect of inorganic arsenics on the seedlings of the rice cultivar Shanyou63. The seedlings were subjected to two treatments, i.e., As(III) and As(V). The results showed that the morphological traits of the seedlings were significantly altered after the arsenic treatments. Analysis of nitrogen, phosphorus, potassium, and arsenic contents of the roots and leaves of the seedlings indicated that the absorption of phosphorus and potassium was mainly affected by As(III), while that of nitrogen was mainly affected by As(V). The expression of 12 genes involved in the absorption and utilization of nitrogen, phosphorus, and potassium were all observed to be down-regulated after the arsenic treatments. As(V) significantly affected the absorption and utilization of nitrogen, while As(III) significantly affected those of phosphorus and potassium. The result obtained by realtime FQ-PCR regarding the difference in the gene expressions agreed with that of our hydroponics experiment. © Franciszek Górski Institute of Plant Physiology.


Fang C.,Key Laboratory of Biopesticide and Chemical Biology | Fang C.,Fujian Agriculture and forestry University | Zhuang Y.,Quanzhou Medical College | Xu T.,Key Laboratory of Biopesticide and Chemical Biology | And 5 more authors.
Journal of Chemical Ecology | Year: 2013

Gene expression of phenylalanine ammonia-lyase (PAL) in allelopathic rice PI312777 was inhibited by RNA interference (RNAi). Transgenic rice showed lower levels of PAL gene expression and PAL activity than wild type rice (WT). The concentrations of phenolic compounds were lower in the root tissues and root exudates of transgenic rice than in those of wild type plants. When barndyardgrass (BYG) was used as the receiver plant, the allelopathic potential of transgenic rice was reduced. The sizes of the bacterial and fungal populations in rice rhizospheric soil at the 3-, 5-, and 7-leaf stages were estimated by using quantitative PCR (qPCR), which showed a decrease in both populations at all stages of leaf development analyzed. However, PI312777 had a larger microbial population than transgenic rice. In addition, in T-RFLP studies, 14 different groups of bacteria were detected in WT and only 6 were detected in transgenic rice. This indicates that there was less rhizospheric bacterial diversity associated with transgenic rice than with WT. These findings collectively suggest that PAL functions as a positive regulator of rice allelopathic potential. © 2013 Springer Science+Business Media New York.


Li Z.,Key Laboratory of Biopesticide and Chemical Biology | Li Z.,Fuzhou University | Qi X.,Fuzhou University | Li Q.,Fuzhou University | And 6 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2010

Obsticals for recropping are especially severe in the production of Rehmannia glutinosa, a Chinese medicinal herb. It taks 8 to 10 years before one can replant a Rehmannia glutinosa to the same field where the crop had been grown. In this study, five different solvents were used to extract potential autotoxins from the soil samples taken from a major Rehmannia glutinosa production area in Jiao-zuo, Henan Province after harvesting the crop. Bioassy was conducted to test the inhibiton rate (IR) of the five soil extracts on Rehmannia glutinosa radicle growth. Soil extract that had the greatest IR was selected for a greenhouse growth study and various physiological and biochemical parameters were measured. Finally, HPLC analysis was used to compare soil extracts with root exudates, and ESI-MS analysis was used to identify the chemical compositions of the soil extracts. Bioassy results demonstrated that water and methanol extracted soil autotoxins had the greatest IR; by adding 0.5 g/mL concentration of soil extracts to a germination study, the IR to Rehmannia glutinosa radicle growth was 17% and 26% for water and methanol extracts, respectively, and the IR reached above 70% when the concentration was increased to 5.0 g/mL. Greenhouse study exhibited that methanol extracted soil autotoxins had resulted in (1) decreased root activities and chlorophyll contents, (2) decreased activities of SOD, CAT, and POD, (3) enhanced membranaceous peroxidation, and (4) decreased growth hormones contents. HPLC analysis showed very similar chromatograms between the methanol extracts and the root exudates. ESI-MS analysis detected six characteristic substances: vanillic acid, D-mannitol, 2[4′-hydroxyphenyl]- ethyl hexacosanoate, verbascoside, β-sitosterol, and daucosterol.


Fang C.-X.,Key Laboratory of Biopesticide and Chemical Biology | Fang C.-X.,Fujian Agriculture and forestry University | He H.-B.,Key Laboratory of Biopesticide and Chemical Biology | He H.-B.,Fujian Agriculture and forestry University | And 12 more authors.
Plant Growth Regulation | Year: 2010

To explore the molecular mechanism of allelopathic rice in response to low nitrogen (N) supply or accompanying weed stress, allelopathic rice PI 312777 and its counterpart Lemont were grown under low N supply or co-cultured with barnyardgrass [Echinochloa crus-galli (L.) Beauv.] in hydroponics. The suppression subtractive hybridization (SSH) technique was employed to isolate the up-regulated genes in the treated rice accession. The results indicated that the expression of the genes associated with N utilization was significantly up-regulated in allelopathic rice PI 312777, and the higher efficiency of N uptake and its utilization were also detected in PI 312777 than that in Lemont when the two rice accessions were exposed to low N supply. This result suggested that the allelopathic rice had higher ability to adapt to low N stress than its non-allelopathic counterpart. However, a different response was observed when the allelopathic rice was exposed to accompanying weed (barnyardgrass) co-cultured in full Hoagland solution (normal N supply). It showed that the expression of the genes associated with allelochemical synthesis and its detoxification were all up-regulated in the allelopathic rice when co-cultured with the target weed under normal N supply. The results suggested that the allelopathic rice should be a better competitor in the rice-weed co-culture system, which could be attributed to increasing de novo biosynthesis and detoxification of allelochemicals in rice, consequently resulting in enhanced allelopathic effect on the target and preventing the autotoxicity in this process. These findings suggested that the accompanying weed, barnyardgrass is not only the stressful factor, but also one of the triggers in activating allelopathy in rice. This implies that the allelopathic rice is sensible of the existing target in chemical communication. © 2010 Springer Science+Business Media B.V.


Lin W.,Key Laboratory of Biopesticide and Chemical Biology | Lin W.,Fujian Agriculture and forestry University | Fang C.,Key Laboratory of Biopesticide and Chemical Biology | Fang C.,Fujian Agriculture and forestry University | And 8 more authors.
Frontiers of Biology in China | Year: 2010

Crop allelopathy is a promising and environmentally friendly method in weed control; however, the inducible genetic trait for allelopathy in the suppression of weeds needs to be overcome for practical use. Further study needs to be directed to this end to elucidate the molecular genetics and its physiologic mechanism. In this paper, the authors review recent advances in the investigation of rice allelopathy and its molecular regulatory mechanism, especially in responses to stressful conditions including biotic and abiotic factors in China. Previous studies show that rice allelopathy could be enhanced when the rice accession was exposed to stressful conditions, and further analysis by the transcriptomics and proteomics approaches conducted in our laboratory indicated that the increase in allelopathic potential of rice, when exposed to the stresses, was attributed to increased expression level of genes involved in phenolic synthetic metabolism. The increasing phenolic compounds have been confirmed as the main allelochemicals and they jointly act to suppress the target, especially in responses to stressful condition, but it seems to be the primary effect in phenolic allelopathy. We still wonder how the exudates from rice root, which were released into rhizosphere soil, are transformed by soil microorganism to produce the higher secondary effect of phenolic allelopathy in the suppression of weeds. Therefore, the authors suggest that rhizosphere biologic properties of allelopathy in rice and its mechanism are being the key research areas in the world now, and systems biology and its approaches, such as metagenomics and metaproteomics, would be helpful to reveal the process and its molecular ecological mechanism regarding rhizospheric biology of rice allelopathy. © 2010 Higher Education Press and Springer-Verlag Berlin Heidelberg.


Xue X.-F.,Nanjing Agricultural University | Luo J.,Key Laboratory of Biopesticide and Chemical Biology | Xu Y.,Key Laboratory of Biopesticide and Chemical Biology | Hong X.-Y.,Nanjing Agricultural University
Zootaxa | Year: 2011

A new mite species belonging to the Eriophyidae (Acari: Eriophyoidea) from Kunming, Yunnan Province, southwestern China, is described and illustrated. Calepitrimerus kunmingensis sp. nov. causes erineum on its host plant, Schefflera odorata (Blanco) Merr. & Rolfe (Araliaceae), commonly known as the umbrella tree. Copyright © 2011 Magnolia Press.

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