Key Laboratory of Biomedical Polymers

Wuhan, China

Key Laboratory of Biomedical Polymers

Wuhan, China
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Lei Q.,Key Laboratory of Biomedical Polymers | Wang S.-B.,Key Laboratory of Biomedical Polymers | Wang S.-B.,Wuhan University | Hu J.-J.,Key Laboratory of Biomedical Polymers | And 5 more authors.
ACS Nano | Year: 2017

In this paper, mesoporous silica nanoparticle (MSN) loaded with doxorubicin (DOX) and capped with tumor-homing/-penetrating peptide tLyP-1-modified tungsten disulfide quantum dots (WS2-HP) was designed and applied as a stimuli-responsive "Cluster Bomb" for high-performance tumor suppression. The peptide tLyP-1 on the surface can both facilitate the homing of DOX@MSN-WS2-HP to 4T1 tumor and greatly enhance the penetration of WS2-HP in tumor. The benzoic-imine bonds as the linkers between "bomblets" and "dispenser" are stable under normal physical conditions and quite labile at pH 6.8. After arriving at the mild acidic tumor microenvironment, the nanoplatform can rapidly break into two parts: (1) electropositive DOX@MSN-NH2 for efficient chemotherapy on surface tumor cells and (2) small-sized WS2-HP with improved tumor penetrating ability for near-infrared (NIR)-light-triggered photothermal therapy (PTT) among deep-seated tumor cells. Having killed the tumor cells in different depths, DOX@MSN-WS2-HP exhibited significant antitumor effect, which will find great potential in clinical trials. © 2017 American Chemical Society.

Liao X.,Hubei University | Liao X.,Key Laboratory of Biomedical Polymers | Gao Z.,Hubei University | Gao Z.,Key Laboratory of Biomedical Polymers | And 6 more authors.
Langmuir | Year: 2017

A series of carboxylate gemini surfactants (CGS, Cn-Φ-Cn, n = 12, 14, 16, 18) with diphenyl ketone as a spacer group were prepared using a simple and feasible synthetic method. These CGS exhibited an excellent surface activity with extremely low critical micelle concentration (CMC) value (approximately 10-5 mol/L), good performance in reducing surface tension (nearly 30 mN/m), and the ability of molecular self-assembly into different aggregate morphologies via adjusting the concentrations, which is attributed to the introduction of diphenyl ketone and carboxylic acid ammonium salt in the molecular structure. Moreover, the surface activity and self-assembly ability of CGS were further optimized by tuning the length of the tail chain. These excellent properties imply that CGS can be a soft template to prepare nanomaterials, especially in morphology-controllable synthesis. By adjusting the concentration of one of CGS (C12-Φ-C12), nano-La2O3 particles with diverse morphologies were obtained, including spherical shape, bead-chain shape, rod shape, velvet-antler shape, cedar shape, and bowknot shape. This work offers a vital insight into the rational design of template agents for the development of morphology-controllable nanomaterials. © 2017 American Chemical Society.

Li Z.-T.,Wuhan University | Guo J.,Wuhan University | Zhang J.-S.,Wuhan Institute of Technology | Zhao Y.-P.,Wuhan University | And 3 more authors.
Carbohydrate Polymers | Year: 2010

A novel chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer with biocleavable disulfide linkages between chitosan chains and PEI grafts was synthesized, characterized, and examined as a potential nonviral gene vector. The chemical structure of the obtained product was characterized by 1H NMR, FTIR and Raman spectroscopy, respectively. Agarose gel retardation assay, dynamic light scattering, and scanning electron microscopy experiments revealed that CHI-g-PEI had a good ability of condensing plasmid DNA into spherical nanoparticles in the size range of 200-300 nm. In the imitative physiological environment the polymer/pDNA complexes are relatively stable, meanwhile, an efficient release of pDNA was detected in the presence of 25 mM DTT, mimicking the intracellular reductive environment. These results show that the bioreducible CHI-g-PEI copolymer, thus obtained, can be used as a promising nonviral gene carrier due to its excellent properties.

Huang Y.,Wuhan University | Zhang L.,Wuhan University | Yang J.,Wuhan University | Yang J.,Key Laboratory of Biomedical Polymers | And 3 more authors.
Macromolecular Materials and Engineering | Year: 2013

Cytocompatible nanocomposite films are prepared by blending α-chitin whiskers and cellulose solution in NaOH/urea. Structure and properties of the chitin/cellulose composite films are characterized by FT-IR, XRD, 13C NMR, SEM, UV-Vis, TGA, and tensile tests. The results reveal that the chitin whiskers are dispersed homogeneously, leading to good miscibility and properties of the chitin/cellulose composite films. By varying the chitin whisker content, the tensile strength and elastic modulus of the films can be controlled. HeLa and T293 cells are seeded onto the surfaces of the nanocomposite films, showing that the composite films were nontoxic to both cell types and that the addition of chitin whiskers promotes cell adhesion and proliferation. Chitin whisker/cellulose nanocomposite films exhibit good miscibility and favorable mechanical properties. The addition of chitin whiskers imrproves the mechanical properties and cytocompatibility of the cellulose matrix significantly. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Zhu Z.,Wuhan University | Zhu Z.,Hubei Key Laboratory on Organic and Polymeric Optoelectronic Materials | Xu L.,Wuhan University | Zhou X.,Wuhan University | And 5 more authors.
Chemical Communications | Year: 2011

Controllable turn off/on fluorescent sensors for Hg2+ detection were developed by designing different label-free DNA sequences and using the minor groove dye of DAPI. These assays exhibit high sensitivity and selectivity, with the detection limits of 5 nM and 1.5 nM for turn off and turn on sensors, respectively. © 2011 The Royal Society of Chemistry.

Yu P.,Key Laboratory of Biomedical Polymers | Xia X.-M.,Key Laboratory of Biomedical Polymers | Wu M.,Key Laboratory of Biomedical Polymers | Cui C.,Key Laboratory of Biomedical Polymers | And 8 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2014

Iron oxide porous nanorods (IOPNR) with lengths ranging from 40nm to 60nm and pore diameters ranging from 5nm to 10nm were prepared, and further modified with NH2-PEG-FA (FA-PEG-IOPNR) for ligand targeting and modified with NH2-PEG-OCH3 (PEG-IOPNR) as a control. Instead of chemical bonding, doxorubicin (DOX), a low water solubility anticancer drug, was loaded in the pores of the modified IOPNR because of their porous structure and high porosity. The release of DOX in acidic PBS solution (pH 5.3) was faster than that in neutral (pH 7.4) solution. The analysis results from TEM, inductively coupled plasma emission spectroscopy, confocal laser scanning microscopy, and flow cytometry analyses indicated that the presence of FA on the surface of the nanorods increase the cellular uptake of nanorods in the case of HeLa cells, a folate receptor (FR)-positive cell line. In contrast, for COS 7 cells, a FR-negative cell line, FA ligand on the surface of the nanorods showed no effect on the cellular uptake. MTT assay indicated that the cytotoxicity of DOX loaded in FA-PEG-IOPNR to HeLa cells was higher than that of DOX in PEG-IOPNR. In the case of COS 7 cells, no significant difference between the cytotoxicity of DOX loaded in FA-PEG-IOPNR and PEG-IOPNR was found. These results suggested that FA-PEG-IOPNR had the potential for target delivery of chemotherapeutic into cancer cells. © 2014 Elsevier B.V.

Zhang L.,Key Laboratory of Biomedical Polymers | Hu C.-H.,Key Laboratory of Biomedical Polymers | Cheng S.-X.,Key Laboratory of Biomedical Polymers | Zhuo R.-X.,Key Laboratory of Biomedical Polymers
Colloids and Surfaces B: Biointerfaces | Year: 2010

Hyperbranched polymers, PG6-PEI25k and PG6-PEI800, were synthesized through grafting branched polyethylenimines (PEIs) with molecular weights of 25 kDa and 800 Da to a polyglycerol core (PG6), respectively. The structure of the polymers was characterized by 1H NMR and FTIR. Through agarose gel electrophoresis retardation assay, PG6-PEI25k and PG6-PEI800 were demonstrated to have capability for DNA binding. PG6-PEI/DNA complexes with different weight ratios were characterized by TEM and particle size analysis. The activity of PG6-PEIs to mediate transfection of reporter plasmids pEGFP-C1 and pGL3-Luc was evaluated on 293T and HeLa cell lines. PG6-PEI25k and PG6-PEI800 showed enhanced levels in transgene expression and decreased cytotoxicities as compared with PEI25k and PEI800, respectively. The results indicated potential applications of PG6-PEIs for efficient gene delivery. © 2009 Elsevier B.V. All rights reserved.

Su W.,Key Laboratory of Biomedical Polymers | Feng J.,Key Laboratory of Biomedical Polymers | Wang H.-F.,Key Laboratory of Biomedical Polymers | Zhang X.-Z.,Key Laboratory of Biomedical Polymers | Zhuo R.-X.,Key Laboratory of Biomedical Polymers
Polymer | Year: 2010

The primary aim of this paper is to explore the influence of the number of CH2 groups per -(CH2)nOCOO- repeat unit (Numc) on the properties of poly(alkylene carbonate)s. A series of poly(alkylene carbonate)s with different Numc, including PTMC (Numc = 3), PTeMC (Numc = 4), PPMC (Numc = 5), PHMC (Numc = 6) and PDMC (Numc = 10) were investigated for that purpose by DSC and XRD techniques. The method of Sn(Oct)2-catalyzed ring-opening polymerization was developed to prepare poly(pentamethylene carbonate)s (PPMC), which presented a controllable feature. Regarding crystallization rate and Tm of poly(alkylene carbonate)s, an interesting odd-even effect was first reported related to the Numc. The order of crystallizing ability of poly(alkylene carbonate) was: PTMC (Numc = 3) < PPMC(Numc = 5) < PTeMC (Numc = 4) < PHMC (Numc = 6) < PDMC (Numc = 10). Poly(alkylene carbonate)s characterized with even Numc appeared to readily crystallize relative to that with odd Numc. © 2010 Elsevier Ltd. All rights reserved.

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