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Luo J.,South China University of Technology | Ling Y.,South China University of Technology | Li X.,South China University of Technology | Yuan B.,Key Laboratory of Biomedical Materials and Engineering | And 4 more authors.
RSC Advances | Year: 2015

The realization of gene therapy significantly depends on effective gene delivery vectors. Bioactive glasses (BG), as osteoinductive bone repair materials have been extensively applied clinically. Although there are numerous studies about the delivery of biomolecules by BG, to date, little study has been done to investigate the impact of BG as an enhancer for polymers/DNA complexes. In our study, we used an MBG with a suitable size to evaluate the influence of CS-PCL-mPEG/pOGP (containing the osteogenic growth peptide and green fluorescent protein fusion gene) on the gene transfection in different types of cell lines. MBG/CS-PCL-mPEG condense and dissociate the pOGP more effectively. These complexes, which are connected by the electrostatic effect, show lower and optimized zeta potential and size below 200 nm. Our study shows that MBG has good cellular biocompatibility and high cell uptake. MBG/CS-PCL-mPEG exhibits enhanced gene transfection efficiency despite the difference between the two cell lines. These results suggest that MBG has an enhanced impact on the polymer gene vectors. Therefore, this strategy may broaden the biomedical application of bioglass in the repair and reconstruction of bone and teeth. © The Royal Society of Chemistry 2015. Source


Li X.,South China University of Technology | Li X.,Key Laboratory of Biomedical Materials and Engineering | Chen X.,South China University of Technology | Chen X.,Key Laboratory of Biomedical Materials and Engineering | And 14 more authors.
Journal of Materials Chemistry B | Year: 2014

Mesoporous bioactive glasses (MBGs) can be used as carriers for biomolecule delivery with improved functions. Although there are a great number of studies on drug delivery by MBGs, until now little work has been done to investigate the DNA gene transfection effect of MBGs. In this study, radial mesoporous bioactive glasses (rMBGs) were prepared by sol-gel process combined with a micro-emulsion method. The surface was further modified by amino groups in order to improve its affinity for DNA. Our study showed that rMBGs have good apatite-forming ability and cellular biocompatibility. In addition, rMBGs can enter cells in a time- and dose-dependent manner, and mainly localize in the cytoplasm. Agarose gel electrophoresis demonstrated that pOA-EGFP (containing the osteoactivin and the green fluorescent protein fusion gene) can be completely absorbed and protected from DNase I degradation by the aminated rMBGs. Additionally, the plasmid can be successfully expressed in cells transfected by rMBGs. This journal is © the Partner Organisations 2014. Source

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