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Han D.,Northeast Agricultural University | Han D.,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Northeast Region | Shi Y.,Northeast Agricultural University | Shi Y.,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Northeast Region | And 9 more authors.
Plant Growth Regulation | Year: 2014

Iron (Fe) is one of the essential micronutrients required by all plants. Citric acid (CA) is considered as the chelate substance in the long distance transport of Fe. In the present study, a gene encoding putative citrate synthase (CS) was isolated from Malusdomestica (L.) Borkh and designated as MdCS1. The MdCS1 gene encodes a protein of 473 amino acid residues with a theoretical isoelectric point of 7.72 and a predicted molecular mass of 52.7 kDa. Subcellular localization has revealed that MdCS1 is preferentially localized in cytoplasmic membrane. The expression of MdCS1 was enriched in leaf, root, and phloem, which was highly affected by Fe stress and indoleacetic acid treatment, while weakly by abscisic acid treatment in M.domestica seedlings. When MdCS1 was introduced into Arabidopsis, it promoted the synthesis of CS and increased CA content. Overexpression of MdCS1 improved the tolerance to Fe stress in transgenic Arabidopsis, but also led to increased fresh weight, CS activity, and contents of chlorophyll, CA and Fe, especially when dealt with Fe stress. © 2014, Springer Science+Business Media Dordrecht. Source

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