Wu J.,China Animal Husbandry Industry Co. |
Wu J.,Key Laboratory of Biological Products and Chemical Drugs for Animals |
Wu J.,China Animal Husbandry Group |
Huang Y.,CAS Institute of Process Engineering |
And 13 more authors.
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2014
Enramycin is a polypeptide antibiotic and new, safe animal feed additive. A new purification process was developed, based on pre-purification by macroporous resin and refining by reversed phase chromatography. AB-8 macroporous resin was used for the pre-purification process of enramycin, with an elution buffer of 0.012 mol/L aqueous HCl solution-methanol (50:50, V/V). Then, enramycin a and enramycin b were separated effectively by C18 reversed phase chromatography, with a elution buffer of 0.05 mol/L aqueous KH2PO4 solution-acetonitrile (70:30, V/V, pH 4.5). The purities of enramycin a and enramycin b were up to 98.5% and 98.0%, respectively. The yield reached 29.2%. This study would provide a useful reference for the preparation of enramycin a and enramycin b with ahigh purity. © 2014 Chin J Biotech, All rights reserved.