Key Laboratory of Biological Products and Chemical Drugs for Animals

Beijing, China

Key Laboratory of Biological Products and Chemical Drugs for Animals

Beijing, China
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Wu J.,China Animal Husbandry Industry Co. | Wu J.,Key Laboratory of Biological Products and Chemical Drugs for Animals | Wu J.,China Animal Husbandry Group | Zhang G.,China Animal Husbandry Industry Co. | And 21 more authors.
Latin American Journal of Pharmacy | Year: 2014

Platenomycin A1 is one type of 16-membered macrolide antibiotics, which had been shown to inhibit the Gram-positive bacteria growth. In this study, the bioconversion of platenomycin A1 from midecamycin in Streptomyces thermotolerans was investigated. With optimized growth conditions, platenomycin A1 production reached to 5.654 g/L. In addition, the platenomycin A1 separation and purification procedures were developed based on pH adjustment and ODS reversed phase chromatography refinement, which leaded to a purity up to 97.74%. Minimum inhibitory concentrations (MIC) of purified platenomycin A1 against representive gram-positive and gram-negative bacteria strains were also determined, which displayed active inhibition against Staphyloccocus aureus (ATCC29213), Streptococcus pneumonia (ATCC49619), septic Streptococcus (CVCC593) and porcine Actinobacillus pleuropneumoniae (CVCC260). © Latin American Journal of Pharmacy 2014. All Rights Reserved.


Wu J.,China Animal Husbandry Industry Co. | Wu J.,Key Laboratory of Biological Products and Chemical Drugs for Animals | Wu J.,China Animal Husbandry Group | Huang Y.,CAS Institute of Process Engineering | And 13 more authors.
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2014

Enramycin is a polypeptide antibiotic and new, safe animal feed additive. A new purification process was developed, based on pre-purification by macroporous resin and refining by reversed phase chromatography. AB-8 macroporous resin was used for the pre-purification process of enramycin, with an elution buffer of 0.012 mol/L aqueous HCl solution-methanol (50:50, V/V). Then, enramycin a and enramycin b were separated effectively by C18 reversed phase chromatography, with a elution buffer of 0.05 mol/L aqueous KH2PO4 solution-acetonitrile (70:30, V/V, pH 4.5). The purities of enramycin a and enramycin b were up to 98.5% and 98.0%, respectively. The yield reached 29.2%. This study would provide a useful reference for the preparation of enramycin a and enramycin b with ahigh purity. © 2014 Chin J Biotech, All rights reserved.

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