Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine

Beijing, China

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine

Beijing, China
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Yi F.,Chinese Academy of Sciences | Yi F.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Sun L.E.,Chinese Academy of Sciences | Sun L.E.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | And 6 more authors.
Pakistan Journal of Botany | Year: 2017

To investigate the phylogenetic relationships among Ilex species distributed in China, we analyzed two alignments including 4,698 characters corresponding to six plastid sequences (matK, rbcL, atpB-rbcL, trnL-F, psbA-trnH, and rpl32-trnL) and 1,748 characters corresponding to two nuclear sequences (ITS and nepGS). Using different partitioning strategies and approaches (i.e., Bayesian inference, maximum likelihood, and maximum parsimony) for phylogeny reconstruction, different topologies and clade supports were determined. A total of 18 Ilex species was divided into two major groups (group I and II) in both plastid and nuclear phylogenies with some incongruences. Potential hybridization events may account, in part, for those phylogenetic uncertainties. The analyses, together with previously identified sequences, indicated that all 18 species were recovered within Eurasia or Asia/North America groups based on plastid data. Meanwhile, the species in group II in the nuclear phylogeny were placed in the Aquifolium clade, as inferred from traditional classification, whereas the species in group I belonged to several other clades. The divergence time of most of the 18 Ilex species was estimated to be not more than 10 million years ago. Based on the results of this study, we concluded that paleogeographical events and past climate changes during the same period might have played important roles in these diversifications. © 2017, Pakistan Botanical Society. All rights reserved.


Xu L.,Peking Union Medical College | Xu L.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Pan H.,Peking Union Medical College | Pan H.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | And 8 more authors.
Food Research International | Year: 2013

Insect tea, listed in the "Compendium of Materia Medica" by Li Shizhen, is not only a traditional drink for the ethnic minority in southwest China, but also one of China's traditional export commodities. Insect tea is made of the feces of insects that feed on plants, and characterized by minimal dose, enjoyable tea flavor, few tea-residues, and superb transparency. Insect tea has been used to clear summer heat, protect the spleen and stomach, and facilitate digestion. Modern research has suggested that insect tea is safe and nutritional, and it has blood lipid lowering, antihypertensive and hypoglycemic effects. At present, due to the household production of insect tea, there are a variety of species of tea-producing insects and feeding plants. In the present review, we summarized the types, civilian applications, nutritional value, pharmacological activity and safety of insect tea, in an effort to provide scientific knowledge for future study. © 2013.


He C.,Peking Union Medical College | He C.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Peng Y.,Peking Union Medical College | Peng Y.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | And 5 more authors.
Zhongguo Zhongyao Zazhi | Year: 2012

Duosuike Tiancha contain multiple dihydrochalcone sweet constituents, which are mainly active constituents. For the purpose of overall assessment on quality Duosuike Tiancha, 5 sweet dihydrochalcones in Duosuike Tiancha, phloridzin, phloretin-4′-β-D-glucopyranoside, 3′-O- acetylphloridzin, 2′-O-acetylphloridzin and phloretin are determined as indicators. The separation was carried out through a isocratic elution using a Waters Acquity UPLC® BRH C 18 (2.1 mm x 100 mm, 1.7 μm) column and a mobile phase consisting of water (75%) and acetonitrile (25%) at a flow rate of 0.5 mL·min -1. The detection wavelength was set at 285 nm. The column temperature was 40°C. Under the optimized conditions, all the 5 sweet constituents were successfully separated with in 6 min, and good linearity (r 2 > 0.9991) was achieved. The linear range (g·L -1) and recoveries were tested with results of 0.0222-0.444 (98.37%), 0.1028-4.112 (97.32%), 0.00339-0.06768 (96.77%), 0.0051-0.204 (98.85%) and 0.000538-0.01076 (100.91%) respectively. The results indicate that the content of the 5 dihydrochalcones were 7.83-62.37, 114.24-272.35, 0-1.02, 0-5.11 and 0.10-1.19 mg·g -1, respectively. Furthermore, with certain regularity between their content and the sample size, harvest time. The separation and analysis method are fast and simple, as evidenced by the fact that the gradient elution is adopted to rapidly determine one sample within six minutes. Therefore, it can be used for dsetermine 5 sweet dihydrochalcones Duosuike Tiancha.


Jiang B.,Peking Union Medical College | Jiang B.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Le L.,Peking Union Medical College | Wan W.,Peking Union Medical College | And 7 more authors.
Endocrinology | Year: 2015

An infusion of Coreopsis tinctoria (CT) flowering tops is traditionally used in Portugal to control hyperglycemia; however, the effects of CT protection against high-fat diet (HFD)-induced hepatic insulin resistance have not been systematically studied and the precise mechanism of action is not clear. The metabolomic profiles of insulin-resistant rats fed a HFD and a CT-supplemented diet (HFD supplemented with CT drinking) for 8 weeks were investigated. Serum samples for clinical biochemistry and liver samples for histopathology and liquid chromatography-mass spectrometry-based metabolomic research were collected. Western blot and quantitative real-time PCR analyses were further used to measure the expression of several relevant enzymes together with perturbed metabolic pathways. Using analysis software, the CT treatment was found to significantly ameliorate the disturbance in 10 metabolic pathways. Combined metabolomic, Western blot, and quantitative real-time PCR analyses revealed that CT treatment significantly improved the glucose homeostasis by, on the one hand, through inhibiting the expression of gluconeogenic pathway key proteins glucose-6-phosphatase and phosphoenolpyruvate carboxykinase and, on the other hand, via regulating the mRNA or protein levels of the Krebs cycle critical enzymes (citrate synthase, succinate dehydrogenase complex, subunit A, flavoprotein, and dihydrolipoamide S-succinyltransferase). These results provide metabolic evidence of the complex pathogenic mechanism involved in hepatic insulin resistance and that the supplementation with CT improves insulin resistance at a global scale. Liquid chromatography-mass spectrometry-based metabolomics approaches are helpful to further understand diabetes-related mechanisms. Copyright © 2015 by the Endocrine Society.


Yi F.,Peking Union Medical College | Yi F.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Tan X.-L.,Peking Union Medical College | Tan X.-L.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | And 3 more authors.
Chinese Medicine (United Kingdom) | Year: 2016

Background: Lepidium meyenii Walpers (maca) is an herb known as a traditional nutritional supplement and widely used in Peru, North America, and Europe to enhance human fertility and treat osteoporosis. The secondary metabolites of maca, namely, maca alkaloids, macaenes, and macamides, are bioactive compounds, but their targets are undefined. Methods: The pharmacophore-based PharmaDB targets database screening joint the ligand shape similarity-based WEGA validation approach is proposed to predict the targets of these unique constituents and was performed using Discovery Studio 4.5 and PharmaDB. A compounds-targets-diseases network was established using Cytoscape 3.2. These suitable targets and their genes were calculated and analyzed using ingenuity pathway analysis and GeneMANIA. Results: Certain targets were identified in osteoporosis (8 targets), prostate cancer (9 targets), and kidney diseases (11 targets). This was the first study to identify the targets of these bioactive compounds in maca for cardiovascular diseases (29 targets). The compound with the most targets (46) was an amide alkaloid (MA-24). Conclusion: In silico target fishing identified maca's traditional effects on treatment and prevention of osteoporosis, prostate cancer, and kidney diseases, and its potential function of treating cardiovascular diseases, as the most important of this herb's possible activities. © 2016 The Author(s).


Sun L.,Peking Union Medical College | Sun L.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Zhang T.,Peking Union Medical College | Zhang T.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | And 11 more authors.
FEBS Letters | Year: 2011

Communication between endothelial and smooth muscle cells (SMCs) contributes to atherosclerosis induced by atherogenic factors, such as oxide LDL. Asymmetric dimethylarginine (ADMA), a newly found cardiovascular risk factor, accumulates in the culture medium of oxide LDL (oxLDL)-treated endothelial cells and positively correlates with atherosclerosis. This study demonstrates that ADMA mediates the communication between endothelial cells and SMCs induced by oxLDL leading to SMC migration. In addition, the present study suggests exogenous ADMA directly induces SMC migration via p38 and ERK1/2 MAPK signaling transduction way. Investigations to identify the factors regulating VSMC migration may provide novel insights into atherosclerosis and its complications. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.


Bi W.,Peking Union Medical College | Bi W.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Shen J.,Peking Union Medical College | Shen J.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | And 7 more authors.
Journal of Functional Foods | Year: 2016

This study provides the scientific proof for Ku-jin tea (Acer tataricum subsp. ginnala or A. tataricum subsp. theiferum) as a functional beverage for the first time. Ku-jin tea is a polyphenol-rich drink and its metabolic profile was analysed using ultra high performance liquid chromatography-diode array detector-electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF-MS). Thirty-five compounds were identified from the extract of Ku-jin tea, including twenty phenolics that are mainly flavonoids and gallotannins. Furthermore, fourteen pure phenolic compounds were isolated from Ku-jin tea and their antioxidant capacities determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays. The results demonstrated that the flavonoids and gallotannins are potent antioxidant compounds, and that the galloyl substituent group could improve the antioxidant activity of the flavonoids. Additionally, quantification of phenolic compounds in Ku-jin tea infusions was carried out with ultra-high performance liquid chromatography (UHPLC). Five gallotannins were found to be predominant, and ginnalin A was determined as the most abundant compound (up to 57.38 mg/g dry weight in most samples). These results revealed that Ku-jin tea could be an excellent functional beverage due to its high concentration of natural antioxidants and active phenolic compounds. © 2016 Elsevier Ltd.


Wang Z.,Peking Union Medical College | Wang Z.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Wang Z.,Changchun University of Chinese Medicine | Ma P.,Peking Union Medical College | And 9 more authors.
Chemistry Central Journal | Year: 2013

Background: Rhubarb is an important Chinese medicinal herb with a long history of over 2000 years and has been commonly used as a laxative. It is the radix and rhizome of Rheum officinale Baill., R. palmatum L. and R. tanguticum Maxim, all of which are mainly distributed in a broad region in the Tibetan plateau. Anthraquinone glycosides are a series of major active ingredients found in all three species. They are key intermediates in the anthraquinone secondary metabolism and the sennnoside biosynthesis. The variation of the anthraquinone glycoside content in rhubarb in response to specific factors remains an attractive topic.Results: A simple and sensitive Ultra Performance Liquid Chromatography with Photo-Diode Array (UPLC-PDA) detector was developed for the simultaneous determination of six anthraquinone glycosides in rhubarb, i.e., aloeemodin-8-O-glucoside, rhein-8-O-glucoside, chrysophanol-1-O-glucoside, emodin-1-O-glucoside, chrysophanol-8-O-glucoside, emodin-8-O-glucoside. Twenty-seven batches from three species were submitted to the multi-component analysis. The results showed that the anthraquinone glycoside content varied significantly even within the same species. The results showed that the anthraquinone glycoside content varied significantly within the same species but not between different species. The PCA and content analysis results confirmed that the plant species has no obvious effect on the content variation. Neither was any significant correlation observed between the anthraquinone glycoside content and the geographic distribution of the rhubarb. Through correlational analysis, altitude was found to be the main factor that affects the anthraquinone glycoside content in rhubarb. Rhubarb grown at higher altitude has higher anthraquinone glycoside content.Conclusions: This work provides a rapid, sensitive and accurate UPLC-PDA method for the simultaneous determination of six anthraquinone glycosides in rhubarb. The anthraquinone glycoside content varied significantly within the same species. The relationship of the anthraquinone glycoside content with plant species, geographic distribution and altitude were studied using correlational analysis, principal component analysis and spatial autocorrelation analysis through SPSS and ArcGIS. Plant species and geographic distribution were found not to affect the content of the six anthraquinone glycosides in rhubarb. The variations in the anthraquinone glycoside content were primarily due to the different altitude where the plant was grown. © 2013 Wang et al.; licensee Chemistry Central Ltd.


Yin W.-Q.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Guo S.-X.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Xing Y.-M.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine | Xing X.-K.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine
Mycological Progress | Year: 2012

Polyporus umbellatus is one of the most valuable medicinal fungi, and its sclerotium has been used as a diuretic agent and an antidote in traditional Chinese medicine. In nature, Polyporus umbellatus has almost been depleted because of over-exploitation and lack of natural habitats. Thus, artificial sclerotia production has increased. This study aimed at finding an effective method to induce sclerotia, and selected the split-plate culture method. One side contained fructose agar medium (FAM), while the other side contained nutrient-limited medium. It was observed that sclerotia were only formed on the nutrient-limited medium side but scarcely emerged on the FAM side, even when the fructose concentration on both sides were the same. The sclerotial differentiation rate was 100% and the sclerotial yield was 106% higher than in the conventional way. In conclusion, the split-plate culture method is an effective way to induce P. umbellatus sclerotia in the laboratory. © 2012 German Mycological Society and Springer.


PubMed | Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine
Type: Journal Article | Journal: Journal of Asian natural products research | Year: 2010

Two new prenylated C(6)-C(3) compounds, 4-epi-illicinone E-12-shikimate (1) and 3-hydroxyillifunone B (2), together with five known prenylated C(6)-C(3) compounds (3-7), were isolated from the fruits of Illicium simonsii. Their structures were elucidated on the basis of extensive spectroscopic methods, including 1D and 2D NMR, CD spectra, and ESI-MS analysis.

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