Wang Z.,Peking Union Medical College |
Wang Z.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine |
Wang Z.,Changchun University of Chinese Medicine |
Ma P.,Peking Union Medical College |
And 9 more authors.
Chemistry Central Journal | Year: 2013
Background: Rhubarb is an important Chinese medicinal herb with a long history of over 2000 years and has been commonly used as a laxative. It is the radix and rhizome of Rheum officinale Baill., R. palmatum L. and R. tanguticum Maxim, all of which are mainly distributed in a broad region in the Tibetan plateau. Anthraquinone glycosides are a series of major active ingredients found in all three species. They are key intermediates in the anthraquinone secondary metabolism and the sennnoside biosynthesis. The variation of the anthraquinone glycoside content in rhubarb in response to specific factors remains an attractive topic.Results: A simple and sensitive Ultra Performance Liquid Chromatography with Photo-Diode Array (UPLC-PDA) detector was developed for the simultaneous determination of six anthraquinone glycosides in rhubarb, i.e., aloeemodin-8-O-glucoside, rhein-8-O-glucoside, chrysophanol-1-O-glucoside, emodin-1-O-glucoside, chrysophanol-8-O-glucoside, emodin-8-O-glucoside. Twenty-seven batches from three species were submitted to the multi-component analysis. The results showed that the anthraquinone glycoside content varied significantly even within the same species. The results showed that the anthraquinone glycoside content varied significantly within the same species but not between different species. The PCA and content analysis results confirmed that the plant species has no obvious effect on the content variation. Neither was any significant correlation observed between the anthraquinone glycoside content and the geographic distribution of the rhubarb. Through correlational analysis, altitude was found to be the main factor that affects the anthraquinone glycoside content in rhubarb. Rhubarb grown at higher altitude has higher anthraquinone glycoside content.Conclusions: This work provides a rapid, sensitive and accurate UPLC-PDA method for the simultaneous determination of six anthraquinone glycosides in rhubarb. The anthraquinone glycoside content varied significantly within the same species. The relationship of the anthraquinone glycoside content with plant species, geographic distribution and altitude were studied using correlational analysis, principal component analysis and spatial autocorrelation analysis through SPSS and ArcGIS. Plant species and geographic distribution were found not to affect the content of the six anthraquinone glycosides in rhubarb. The variations in the anthraquinone glycoside content were primarily due to the different altitude where the plant was grown. © 2013 Wang et al.; licensee Chemistry Central Ltd. Source
Xu J.-S.,Peking Union Medical College |
Xu J.-S.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine |
Wei J.-H.,Peking Union Medical College |
Wei J.-H.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine |
And 6 more authors.
Chinese Traditional and Herbal Drugs | Year: 2013
Objective: To clone the full-length cDNA of the uridine diphosphate glycosyltransferase (UGT) gene in Bupleurum chinense (BcUGT8), which may be involved with the saikosaponin biosynthesis, and to construct the prokaryotic expression vector. The work will provide the foundation for its further function verification by in vitro expression and activity analysis of the purified protein. Methods: RACE and LD-PCR were used to clone the full-length cDNA of BcUGT8, on the basis of its partial cDNA sequence obtained from our previous high-flux sequencing by Roche (454) GS FLX system. The open reading form (ORF) was PCR cloned using primers with corresponding restriction enzymes cutting sites. The PCR products were digested with corresponding restriction enzymes and then were inserted in expression vector pET-28a (+) to construct the recombinant expression vectors. Results: The full-length cDNA of UGT gene was cloned from B. chinense, and the prokaryotic expression vector was obtained. Conclusion: The full-length cDNA cloning, sequence analysis, and prokaryotic expression vector construction provide a substantial foundation for follow-up bio-function analysis of BcUGT8 through protein expression, purification, and activity analysis in vitro. Source
He C.,Peking Union Medical College |
He C.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine |
Peng Y.,Peking Union Medical College |
Peng Y.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine |
And 5 more authors.
Zhongguo Zhongyao Zazhi | Year: 2012
Duosuike Tiancha contain multiple dihydrochalcone sweet constituents, which are mainly active constituents. For the purpose of overall assessment on quality Duosuike Tiancha, 5 sweet dihydrochalcones in Duosuike Tiancha, phloridzin, phloretin-4′-β-D-glucopyranoside, 3′-O- acetylphloridzin, 2′-O-acetylphloridzin and phloretin are determined as indicators. The separation was carried out through a isocratic elution using a Waters Acquity UPLC® BRH C 18 (2.1 mm x 100 mm, 1.7 μm) column and a mobile phase consisting of water (75%) and acetonitrile (25%) at a flow rate of 0.5 mL·min -1. The detection wavelength was set at 285 nm. The column temperature was 40°C. Under the optimized conditions, all the 5 sweet constituents were successfully separated with in 6 min, and good linearity (r 2 > 0.9991) was achieved. The linear range (g·L -1) and recoveries were tested with results of 0.0222-0.444 (98.37%), 0.1028-4.112 (97.32%), 0.00339-0.06768 (96.77%), 0.0051-0.204 (98.85%) and 0.000538-0.01076 (100.91%) respectively. The results indicate that the content of the 5 dihydrochalcones were 7.83-62.37, 114.24-272.35, 0-1.02, 0-5.11 and 0.10-1.19 mg·g -1, respectively. Furthermore, with certain regularity between their content and the sample size, harvest time. The separation and analysis method are fast and simple, as evidenced by the fact that the gradient elution is adopted to rapidly determine one sample within six minutes. Therefore, it can be used for dsetermine 5 sweet dihydrochalcones Duosuike Tiancha. Source
Bi W.,Peking Union Medical College |
Bi W.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine |
Shen J.,Peking Union Medical College |
Shen J.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine |
And 7 more authors.
Journal of Functional Foods | Year: 2016
This study provides the scientific proof for Ku-jin tea (Acer tataricum subsp. ginnala or A. tataricum subsp. theiferum) as a functional beverage for the first time. Ku-jin tea is a polyphenol-rich drink and its metabolic profile was analysed using ultra high performance liquid chromatography-diode array detector-electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF-MS). Thirty-five compounds were identified from the extract of Ku-jin tea, including twenty phenolics that are mainly flavonoids and gallotannins. Furthermore, fourteen pure phenolic compounds were isolated from Ku-jin tea and their antioxidant capacities determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays. The results demonstrated that the flavonoids and gallotannins are potent antioxidant compounds, and that the galloyl substituent group could improve the antioxidant activity of the flavonoids. Additionally, quantification of phenolic compounds in Ku-jin tea infusions was carried out with ultra-high performance liquid chromatography (UHPLC). Five gallotannins were found to be predominant, and ginnalin A was determined as the most abundant compound (up to 57.38 mg/g dry weight in most samples). These results revealed that Ku-jin tea could be an excellent functional beverage due to its high concentration of natural antioxidants and active phenolic compounds. © 2016 Elsevier Ltd. Source
Sun L.,Peking Union Medical College |
Sun L.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine |
Zhang T.,Peking Union Medical College |
Zhang T.,Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine |
And 11 more authors.
FEBS Letters | Year: 2011
Communication between endothelial and smooth muscle cells (SMCs) contributes to atherosclerosis induced by atherogenic factors, such as oxide LDL. Asymmetric dimethylarginine (ADMA), a newly found cardiovascular risk factor, accumulates in the culture medium of oxide LDL (oxLDL)-treated endothelial cells and positively correlates with atherosclerosis. This study demonstrates that ADMA mediates the communication between endothelial cells and SMCs induced by oxLDL leading to SMC migration. In addition, the present study suggests exogenous ADMA directly induces SMC migration via p38 and ERK1/2 MAPK signaling transduction way. Investigations to identify the factors regulating VSMC migration may provide novel insights into atherosclerosis and its complications. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Source