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Kong L.-H.,Sichuan University | Kong L.-H.,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province | Kong L.-H.,Key Laboratory of Bio resources and Eco environment | Lei C.-W.,Sichuan University | And 32 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2017

Sixteen different sequence types (STs) of Escherichia coli isolates from a commercial swine farm in China were confirmed to coharbor the carbapenem resistance gene blaNDM-5 and the colistin resistance gene mcr-1. Whole-genome sequencing revealed that blaNDM-5 and mcr-1 were located on a 46-kb IncX3 plasmid and a 32-kb IncX4 plasmid, respectively. The two plasmids can transfer together with a low fitness cost, which might explain the presence of various STs of E. coli coharboring blaNDM-5 and mcr-1. © 2017 American Society for Microbiology. All Rights Reserved.


Shi Z.,Key Laboratory of Bio resources and Eco environment | Shi Z.,Guizhou Normal University | Chen J.,Sichuan Academy of Medical Science | Li C.-Y.,Key Laboratory of Bio resources and Eco environment | And 5 more authors.
Acta Pharmacologica Sinica | Year: 2014

Aim: Proteins with legume lectin domains are known to possess a wide range of biological functions. Here, the antitumor effects of two representative legume lectins, concanavalin A (ConA) and Sophora flavescens lectin (SFL), on human breast carcinoma cells were investigated in vitro and in vivo.Methods:Human breast carcinoma MCF-7 cells and human normal mammary epithelial MCF-10A cells were examined. Cell viability was detected using WST-1 and CCK-8 assays. Cell apoptosis was analyzed with Hoechst 33258 staining. Cell cycle was investigated using flow cytometry. The expression of relevant proteins was measured using Western blotting. Breast carcinoma MCF-7 bearing nude mice were used to study the antitumor effects in vivo. The mice were injected with ConA (40 mg/kg, ip) and SFL (55 mg/kg, ip) daily for 14 d.Results:ConA and SFL inhibited the growth of MCF-7 cells in dose- and time-dependent manners (IC 50 values were 15 and 20 μg/mL, respectively). Both ConA and SFL induced apoptotic morphology in MCF-7 cells without affecting MCF-10A cells. ConA and SFL dose-dependently increased the sub-G 1 proportion in MCF-7 cells, while SFL also triggered the G 2 /M phase cell cycle arrest. Both ConA and SFL dose-dependently increased the activities of caspase-3 and caspase-9 and release of cytochrome C from mitochondria into cytoplasm, up-regulated Bax and Bid, and down-regulated Bcl-2 and Bcl-X L in MCF-7 cells. ConA reduced NF-κB, ERK, and JNK levels, and increased p53 and p21 levels, while SFL caused similar changes in NF-κB, ERK, p53, and p21 levels, but did not affect JNK expression. Administration of ConA and SFL significantly decreased the subcutaneous tumor mass volume and weight in MCF-7 bearing nude mice.Conclusion:ConA and SFL exert anti-tumor actions against human breast carcinoma MCF-7 cells both in vitro and in vivo.


Long F.,Sichuan University | Long F.,Key Laboratory of Bio resources and Eco environment | Li S.,Sichuan University | Li S.,State Key Laboratory of Hydraulics and Mountain River Engineering | And 3 more authors.
Journal of Mountain Science | Year: 2012

Field pull-out and laboratory tests were applied to investigate the effect of metal bolts and wire netting on plant root distribution and anchorage characteristics of 9-year-old Vitex negundo L. growing on a weakly weathered rocky slope (38°). Root number, length and diameter were recorded and single root specimens were classified and tested for tensile strength. The results show that root anchorage is different between the two constructions by bolts and wire. Under limited soil condition, root anchorage ability is 80% lower than when there is sufficient soil due to the reduction of the diameter and number of all second-order lateral roots, as well as the reduction of the tensile strength of the embedded second-order and first-order lateral roots on limited soil. We conclude that the distribution and anchorage of Vitex negundo L. is affected by limited soil availability on rocky slopes. © 2012 Science Press, Institute of Mountain Hazards and Environment, CAS and Springer-Verlag Berlin Heidelberg.


Li X.Y.,Sichuan University | Li X.Y.,Bio Resource Research and Utilization Joint Key Laboratory of Sichuan and Chongqing | Li X.Y.,Key Laboratory of Bio Resources and Eco Environment | Ye J.Z.,Sichuan University | And 17 more authors.
Genetics and Molecular Research | Year: 2015

We examined the association between the methionine synthase reductase (MTRR A66G), methylenetetrahydrofolate reductase (MTHFR C677T and A1298C), and methionine synthase (MS A2756G) genotypes and non-obstructive male infertility in a Chinese population. This case-control study included 162 infertile Chinese patients with azoospermia (N = 100) or oligoasthenozoospermia (N = 62) and 120 fertile men as controls. The polymorphisms MTRR A66G, MTHFR C677T, A1298C, and MS A2756G were identified by direct DNA sequencing and the results were statistically analyzed. We found no association between the incidence of any of these variants in azoospermia patients and control populations. The frequency of the MTRR66 polymorphic genotypes (AG, AG+GG) was significantly higher in the oligoasthenozoospermia group compared to the controls (P = 0.013, 0.012). Our findings revealed an association between the single-nucleotide polymorphism A66G in the MTRR gene and male infertility, particularly in oligoasthenozoospermia males, suggesting that this polymorphism is a genetic risk factor for male infertility in Chinese men. © FUNPEC-RP.


Zhang X.,Sichuan University | Zhang X.,Bio Resource Research and Utilization Joint Key Laboratory of Sichuan and Chongqing | Zhang X.,Key Laboratory of Bio Resources and Eco Environment | Ding M.,Sichuan University | And 11 more authors.
Systems Biology in Reproductive Medicine | Year: 2015

Four genes involved in DNA double-strand break repair and chromosome synapsis, i.e., testis expressed gene 11 (TEX11), testis expressed gene 15 (TEX15), mutL homolog 1 (MLH1), and homolog 3 (MLH3), play critical roles in genome integrity, meiotic recombination, and gametogenesis. We explored the possible association between single nucleotide polymorphisms (SNPs) in these genes and idiopathic male infertility involving azoospermia or oligozoospermia. A total of 614 fertile control and infertile men were recruited to this study in Sichuan, China. The latter group included 244 men with azoospermia and 72 men with oligozoospermia. Six SNPs in the TEX11, TEX15, MLH1, and MLH3 genes were investigated in both patients and controls by sequencing. The frequency distributions of SNPs rs6525433, rs175080, rs6525433-rs4844247, and rs1800734-rs175080 were found to be significantly different between patients and control groups (p<0.05), while rs4844247, rs323344, rs323346, and rs1800734 showed no significant difference between the two cohorts. Thus, the SNPs TEX11 rs6525433, MLH3 rs175080, rs6525433-rs4844247, and rs1800734-rs175080 might be associated with male infertility. © 2015 Informa Healthcare USA, Inc. All rights reserved.


Liu M.,Key Laboratory of Bio resources and Eco environment | Kang C.,Key Laboratory of Bio resources and Eco environment | Yan C.,Key Laboratory of Bio resources and Eco environment | Huang T.,Key Laboratory of Bio resources and Eco environment | And 4 more authors.
Mitochondrial DNA | Year: 2016

The Black Stork, Ciconia nigra belongs to family Ciconiidae, which is evaluated as Least Concern by IUCN. In this study, the complete mitochondrial genome of C. nigra was first sequenced and characterized, which was 17,795 bp in length. The mt-genome has tandem repeats of 80 bp and 78 bp repeat units, and AAACAAC and AAACAAACAAC tandem repeats in D-loop region. It is notable that a single extra base "C" at position 174 was inserted in gene ND3. Bayesian inference, maximum likelihood methods were used to construct phylogenetic trees based on 12 heavy-strand protein-coding genes. Phylogenetic analyses showed that Ardeidae diverged earlier than Ciconiidae, Cathartida and Threskiornithidae, and Ciconiidae had closest relationship to Cathartida. C. nigra diverged first among three Ciconia birds. © 2014 Informa UK Ltd.


Lei C.-W.,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province | Lei C.-W.,Key Laboratory of Bio resources and Eco environment | Lei C.-W.,Sichuan University | Zhang A.-Y.,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province | And 22 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2015

Four different Salmonella genomic island 1 (SGI1) variants, including two novel variants, were characterized in one Salmonella enterica serovar Rissen sequence type ST1917 isolate and three Proteus mirabilis isolates from swine farms in China. One novel variant was derived from SGI1-B with the backbone gene S021 disrupted by a 12.72-kb IS26 composite transposon containing the dfrA17-aadA5 cassettes and macrolide inactivation gene cluster mphA-mrx-mphR. The other one was an integron-free SGI1 and contained a 183-bp truncated S025 next to IS6100 and S044. Copyright © 2015, American Society for Microbiology. All Rights Reserved.


Zhang A.,Sichuan University | Zhang A.,Key Laboratory of Bio resources and Eco environment | Yang Y.,Sichuan University | Wang H.,Sichuan University | And 13 more authors.
Journal of Wildlife Diseases | Year: 2015

To determine the antimicrobial susceptibility profiles and prevalence of resistance genes in Escherichia coli isolated from yaks (Bos grunniens) and herdsmen in nine plateau pastures in Tibet, we isolated 184 nonidentical strains of E. coli from yaks and herdsmen. Antimicrobial susceptibility testing of 15 antimicrobials was conducted and the prevalence of sulfonamide resistance genes (sul1, sul2, and sul3) and florfenicol resistance genes (floR, cfr, cmlA, fexA, pexA, and estDL136) was determined. Escherichia coli isolated from yaks had a high resistance rate to sulfamethoxazole (44%), sulphafurazole (40.4%), and florfenicol (11.4%). Escherichia coli isolated from herdsmen had a high resistance rate to sulfamethoxazole (57%) and sulphafurazole (51%). In addition, sul genes were present in 93% of sulfonamide-resistant isolates (84/90), and 17 floR genes and four cmlA genes were found in 19 florfenicol-resistant isolates. Even though florfenicol is prohibited from use in humans, three floR genes were detected in strains isolated from herdsmen. The three floR-positive isolates from herdsmen had pulsed-field gel electrophoresis patterns similar to isolates from yaks. In addition to documenting the sul and floR genes in E. coli isolated from yaks and herdsmen in the Tibetan pasture, we demonstrated the potential risk that antimicrobial-resistant E. coli could spread among herdsmen and yaks. © Wildlife Disease Association 2015.


Wang Y.,University of Sichuan | Gu R.,University of Sichuan | Chen H.,University of Sichuan | Shi H.,University of Sichuan | And 6 more authors.
Israel Journal of Plant Sciences | Year: 2015

The male sterility system shows tremendous value for the long-term utilization of hybrid crop breeding. In this study, a male sterile mutant, K305ms, was derived from the M3 progeny of maize (Zea mays L.) inbred line K305 exposed to 60Co-γ irradiation. Male sterile K305ms plants did not show any obvious differences from their sibling male fertile plants (K305F) during the vegetative stage, but failed to produce functional pollen at the reproductive stage. Microscopic observations determined that the dyads and tetrads from the pollen of K305ms plants developed abnormally, and subsequently the microspores were shriveled. Genetic analysis indicated that the male sterility of K305ms was controlled by a single recessive genic gene. Gene mapping showed that the responsible gene was located between two simple sequence repeat markers on chromosome 2L in a region of 10.3 cM, bnlg469b and bnlg1940, with genetic distances of 2.9 and 7.4 cM, respectively. According to the microscopic and mapping characteristics, our results showed that this gene was distinguishable from all other reported male sterile genes in maize, and it is temporarily designated as ms305. The linkage map in this study will provide a useful fundamental basis for molecular marker-assisted selection as well as for further map-based cloning of ms305. © 2015 2015 Taylor © Francis.


Tian G.-B.,University of Sichuan | Tian G.-B.,Key Laboratory of Bio Resources and Eco Environment | Wang H.-N.,University of Sichuan | Wang H.-N.,Key Laboratory of Bio Resources and Eco Environment | And 14 more authors.
Journal of Medical Microbiology | Year: 2012

Data correlating β-lactamases found in commensal Escherichia coli of human and animal origin are limited. In this study, 447 commensal E. coli isolates from the faeces of humans and swine (280 human isolates from four hospitals and 167 swine isolates from seven farms) were collected between September 2006 and January 2009 in western China. For extended-spectrum β-lactamase (ESBL)-producing and other cephalosporin-resistant isolates, the relevant β-lactamase genes (blaTEM, blaSHV, blaCTX-M-1/2/9 group, blaCMY-2 and blaKPC) were detected by PCR analysis. Of the 447 isolates tested, 120 (26.8%) were confirmed as producing ESBL. Among these, 70 and 40 human isolates carried a member of the blaCTX-M-1 group (13 blaCTX-M-3, 21 blaCTX-M-15, four blaCTX-M-22, eight blaCTX-M-28, four blaCTX-M-36, 15 blaCTX-M-55 and five blaCTX-M-69) or blaSHV (14 blaSHV-2, seven blaSHV-5, ten blaSHV-12, five blaSHV-57 and four blaSHV-97), respectively, whilst six and four swine isolates carried a member of the blaCTX-M-1 group (one blaCTX-M-15 and five blaCTX-M-22) or blaSHV (three blaSHV-2 and one blaSHV-12), respectively. Furthermore, 59 human and swine isolates and seven human isolates carried blaCMY-2 and blaKPC, respectively. These findings indicate that the blaCTX-M-1 group, including the novel variant blaCTX-M-69, and blaSHV are the predominant ESBL genes in both humans and swine in western China, and blaCMY-2 is also common in both groups. The carriage rates of broad-spectrum β-lactamases among commensal E. coli was much lower in swine than in humans, suggesting that β-lactamase genes have not established themselves in animal ecosystems in western China. © 2012 SGM.

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