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Huo J.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Wang P.,Yunnan Agricultural University | Wei H.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Zeng Y.,Yunnan Agricultural University
Journal of Animal and Veterinary Advances | Year: 2012

The complete coding sequence of Banna Mini-pig Inbred line (BMI) gene PQBP1 was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to accession number JF750401. Sequence analysis revealed that the BMI PQBP1 encodes a protein of 265 ammo acids that has high homology with the Polyglutamme Binding Proteinl (PQBP1) of five other species-human (95%), monkey (95%), cattle (95%), mouse (88%) and rat (87%). The phylogenetic tree analysis revealed BMI PQBP1 has a closer genetic relationship with the cattle PQBP1 than with those of human, monkey, mouse and rat. Analysis by RT-PCR showed that BMI PQBP1 gene was over-expressed in midbrain, ovary, spleen, lung, nerve fiber, skin and muscle, moderately expressed in diencephalon, kidney, heart, stomach, small intestine, large intestine and fat, weakly expressed in lymph node and cerebrum and almost not expressed in liver and pancreas. One microRNA target site was predicted in the CDS of BMI PQBP1 mRNA for further studying this gene in the future. These data serve as a foundation for further insight into this swine gene. © 2012 Medwell Journals. Source


Jinlong H.,Yunnan University | Jinlong H.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Jinlong H.,Yunnan Agricultural University | Hailong H.,Yunnan University | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

In 1980, the Banna Mini-pig Inbred line was established in China. The original ancestors were a sow and her son with the same black color coats. The propagation was conducted by full sibling or parent-offspring mating. With the development of inbreeding, white with black spotting individuals were generated. In the study, a principal coat color encoded candidate gene of MCIR was studied from the typical 8 generation of BMI for revealing the genetic mechanism. It was shown that BMI owned two MCIR alleles named E BMI and E bmi, corresponding to EU604026 andEU604027 in GenBank. Genotypes of E bmi/E bmi and E bmi/E bmi were black color while the white with black spotting phenotype owned the E bmi/E bmi genotype. The E bmi with the length of 963 bp single-coding exon encodes 320 amino acids. Compared with the E BMI sequence, 2 bp was inserted in the 66th nucleotide position of the E bmi encoding regions. The insertion caused a frameshift mutation that introduced a premature stop at codon 55, encoding 54 amino acids. It was indicated that the MCIR gene played a key role in the genetic process of the BMI coat color and the dominant of BMI black phenotype to white with black spotting phenotype was confirmed by the combination the pedigree phenotype deduction and genotype testing. © Medwell Journals, 2012. Source


Huo J.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Huo J.,Yunnan Agricultural University | Huo J.,Yunnan University | Wang P.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | And 5 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

The complete CDS sequence of Banna Mini-pig Inbred line (BMI) gene FAIM1 was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to Accession No.: JF271685. Sequence analysis revealed that the BMI FAIM1 encodes a protein of 200 amino acids that has high homology with the Fas apoptosis inhibitory molecule 1 proteins of five other species-cattle 95%, horse 95%, human 94%, mouse 92% and rat 91 %. The phylogenetic tree analysis revealed BMI FAIM1 has a closer genetic relationship with the bovine FAIM1 than with those of horse, human, mouse and rat. Analysis by RT-PCR showed that BMI FAIM1 gene was over-expressed in lymph node, diencephalon, heart, muscle, moderately expressed in midbrain, spleen, lung, small intestine, fat and almost not expressed in other 9 tissues. Several microRNA target sites were predicted in the CDS of BMI FAIM1 mRNA for further studying this gene in the future. © Medwell Journals, 2012. Source


Wang P.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Wang P.,Yunnan Agricultural University | Huo J.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Huo J.,Yunnan Agricultural University | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

The complete CDS sequence of Banna Mini-pig Inbred line (BMI) gene FAR1 was amplified using the Reverse Transcriptase-polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to Accession No.: JF944893. Sequence analysis revealed that the BMI FAR1 encodes a protein of 515 ammo acids that has high homology with the fatty acyl-CoA reductase 1 proteins of seven species-cattle 98%, horse 98%, mouse 98%, orangutan 97%, human 97%, monkey 97% and rat 92%. The phylogenetic tree analysis revealed BMI FAR1 has a closer genetic relationship with the bovine, human, orangutan and monkey FAR1 than with those of horse, mouse and rat. Analysis by RT-PCR showed that BMI FAR1 gene was over-expressed in muscle, lung, ovary, skin, large intestine, spleen, small intestine and nerve fiber and almost not expressed in other 10 tissues. Several microRNA target sites were predicted in the CDS of BMI FAR1 mRNA for further studying this gene in the future. The 3D structure of the FAR1 by homology modeling was similar to that of pseudomonas 3-alpha-hydroxysteroid dehydrogenase (2 dkn Chain: A). The experiment will establish a foundation for further insight into this swine gene. © Medwell Journals, 2012. Source


Wang P.,Yunnan University | Wang P.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Wang P.,Yunnan Agricultural University | Huo H.L.,Yunnan University | And 13 more authors.
Genetics and Molecular Research | Year: 2015

Testis-specific serine kinases (TSSKs) are a family of serine/threonine kinases highly expressed in the testes that are responsible for regulating many spermatogenesis-related protein activities. Mutations in this family have a positive relationship with oligospermia and azoospermia in human and mouse. Here, five members of the TSSK family from a Banna mini-pig inbred line (BMI) were cloned, sequenced, and characterized. The full-length coding sequences of BMI TSSKs varied from 807 (TSSK3) to 1095 bp (TSSK1) and encoded 268 to 364 amino acids with molecular weights in the range 30.11 to 41.34 kDa. Following comparison with TSSK4 genes in other species, BMI TSSK4 was found to contain three alternatively spliced variants, inform1, inform 3, and inform 4. BMI TSSK1 and TSSK2 are co-localized on the Sus scrofa chromosome (SSC) 14, and consist of a single exon; TSSK3, TSSK4, and TSSK6 are on SSC6, SSC7, and SSC2, and consist of two, four, and one exon, respectively. Multiple protein sequence alignment and phylogenetic analysis showed that the regions spanning the S_TKc domains were more conserved between pig and other animals: with TSSK1 and TSSK2 and TSSK3 and TSSK6 displaying the greatest degree of homology across species, and the TSSK4 protein clearly distinct from other members. Multi-tissue RT-PCR showed BMI TSSK1, TSSK3, and TSSK4 were only expressed in the testes and seminal vesicle, TSSK2 was confined to testes only, while TSSK6 was expressed widely in adult tissues but was highest in the testes. © FUNPEC-RP. Source

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