Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province

Kunming, China

Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province

Kunming, China
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Huo J.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Huo J.,Yunnan Agricultural University | Huo J.,Yunnan University | Wang P.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | And 5 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

The complete CDS sequence of Banna Mini-pig Inbred line (BMI) gene FAIM1 was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to Accession No.: JF271685. Sequence analysis revealed that the BMI FAIM1 encodes a protein of 200 amino acids that has high homology with the Fas apoptosis inhibitory molecule 1 proteins of five other species-cattle 95%, horse 95%, human 94%, mouse 92% and rat 91 %. The phylogenetic tree analysis revealed BMI FAIM1 has a closer genetic relationship with the bovine FAIM1 than with those of horse, human, mouse and rat. Analysis by RT-PCR showed that BMI FAIM1 gene was over-expressed in lymph node, diencephalon, heart, muscle, moderately expressed in midbrain, spleen, lung, small intestine, fat and almost not expressed in other 9 tissues. Several microRNA target sites were predicted in the CDS of BMI FAIM1 mRNA for further studying this gene in the future. © Medwell Journals, 2012.


Jinlong H.,Yunnan University | Jinlong H.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Jinlong H.,Yunnan Agricultural University | Hailong H.,Yunnan University | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

In 1980, the Banna Mini-pig Inbred line was established in China. The original ancestors were a sow and her son with the same black color coats. The propagation was conducted by full sibling or parent-offspring mating. With the development of inbreeding, white with black spotting individuals were generated. In the study, a principal coat color encoded candidate gene of MCIR was studied from the typical 8 generation of BMI for revealing the genetic mechanism. It was shown that BMI owned two MCIR alleles named E BMI and E bmi, corresponding to EU604026 andEU604027 in GenBank. Genotypes of E bmi/E bmi and E bmi/E bmi were black color while the white with black spotting phenotype owned the E bmi/E bmi genotype. The E bmi with the length of 963 bp single-coding exon encodes 320 amino acids. Compared with the E BMI sequence, 2 bp was inserted in the 66th nucleotide position of the E bmi encoding regions. The insertion caused a frameshift mutation that introduced a premature stop at codon 55, encoding 54 amino acids. It was indicated that the MCIR gene played a key role in the genetic process of the BMI coat color and the dominant of BMI black phenotype to white with black spotting phenotype was confirmed by the combination the pedigree phenotype deduction and genotype testing. © Medwell Journals, 2012.


Huo J.,Yunnan University | Huo J.,Key Laboratory of Banna Mini pig Inbred Line of Yunnan Province | Huo J.,Yunnan Agricultural University | Wang P.,Key Laboratory of Banna Mini pig Inbred Line of Yunnan Province | And 6 more authors.
African Journal of Biotechnology | Year: 2012

The complete expressed sequence tag (CDS) sequence of Banna mini-pig inbred line (BMI) ribokinase gene (RBKS) was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to accession number JF944892. Sequence analysis revealed that the BMI RBKS encodes a protein of 323 amino acids that has high homology with the ribokinase proteins of seven species: cattle (99%), horse (99%), orangutan (99%), human (89%), monkey (89%), rat (88%) and mouse (80%). The phylogenetic tree analysis revealed that the BMI RBKS gene has a closer genetic relationship with the RBKS genes of bovine and horse than with those of orangutan, human, monkey, rat and mouse. Analysis by RT-PCR showed that BMI RBKS gene was over-expressed in ovary and lung, moderately expressed in spleen, nerve fiber, large intestine and diencephalon, weakly expressed in heart, skin, muscle, small intestine, midbrain, kidney and fat, while almost silent in other five tissues. Four microRNA target sites were predicted in the CDS of BMI RBKS mRNA for further study of this gene in the future. The 3D structure of the RBKS by homology modeling was similar to that of human ribokinase (2fv7). Our experiment will establish a foundation for further insight into this swine gene. © 2012 Academic Journals.


PubMed | Jilin University, Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province, Yunnan Agricultural University, Yunnan University and Neijiang Vocational & Technical College
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Testis-specific serine kinases (TSSKs) are a family of serine/threonine kinases highly expressed in the testes that are responsible for regulating many spermatogenesis-related protein activities. Mutations in this family have a positive relationship with oligospermia and azoospermia in human and mouse. Here, five members of the TSSK family from a Banna mini-pig inbred line (BMI) were cloned, sequenced, and characterized. The full-length coding sequences of BMI TSSKs varied from 807 (TSSK3) to 1095 bp (TSSK1) and encoded 268 to 364 amino acids with molecular weights in the range 30.11 to 41.34 kDa. Following comparison with TSSK4 genes in other species, BMI TSSK4 was found to contain three alternatively spliced variants, inform1, inform 3, and inform 4. BMI TSSK1 and TSSK2 are co-localized on the Sus scrofa chromosome (SSC) 14, and consist of a single exon; TSSK3, TSSK4, and TSSK6 are on SSC6, SSC7, and SSC2, and consist of two, four, and one exon, respectively. Multiple protein sequence alignment and phylogenetic analysis showed that the regions spanning the S_TKc domains were more conserved between pig and other animals: with TSSK1 and TSSK2 and TSSK3 and TSSK6 displaying the greatest degree of homology across species, and the TSSK4 protein clearly distinct from other members. Multi-tissue RT-PCR showed BMI TSSK1, TSSK3, and TSSK4 were only expressed in the testes and seminal vesicle, TSSK2 was confined to testes only, while TSSK6 was expressed widely in adult tissues but was highest in the testes.


Huo J.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Wang P.,Yunnan Agricultural University | Wei H.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Zeng Y.,Yunnan Agricultural University
Journal of Animal and Veterinary Advances | Year: 2012

The complete coding sequence of Banna Mini-pig Inbred line (BMI) gene PQBP1 was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to accession number JF750401. Sequence analysis revealed that the BMI PQBP1 encodes a protein of 265 ammo acids that has high homology with the Polyglutamme Binding Proteinl (PQBP1) of five other species-human (95%), monkey (95%), cattle (95%), mouse (88%) and rat (87%). The phylogenetic tree analysis revealed BMI PQBP1 has a closer genetic relationship with the cattle PQBP1 than with those of human, monkey, mouse and rat. Analysis by RT-PCR showed that BMI PQBP1 gene was over-expressed in midbrain, ovary, spleen, lung, nerve fiber, skin and muscle, moderately expressed in diencephalon, kidney, heart, stomach, small intestine, large intestine and fat, weakly expressed in lymph node and cerebrum and almost not expressed in liver and pancreas. One microRNA target site was predicted in the CDS of BMI PQBP1 mRNA for further studying this gene in the future. These data serve as a foundation for further insight into this swine gene. © 2012 Medwell Journals.


Wang P.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Wang P.,Yunnan Agricultural University | Huo J.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Huo J.,Yunnan Agricultural University | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

The complete CDS sequence of Banna Mini-pig Inbred line (BMI) gene FAR1 was amplified using the Reverse Transcriptase-polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to Accession No.: JF944893. Sequence analysis revealed that the BMI FAR1 encodes a protein of 515 ammo acids that has high homology with the fatty acyl-CoA reductase 1 proteins of seven species-cattle 98%, horse 98%, mouse 98%, orangutan 97%, human 97%, monkey 97% and rat 92%. The phylogenetic tree analysis revealed BMI FAR1 has a closer genetic relationship with the bovine, human, orangutan and monkey FAR1 than with those of horse, mouse and rat. Analysis by RT-PCR showed that BMI FAR1 gene was over-expressed in muscle, lung, ovary, skin, large intestine, spleen, small intestine and nerve fiber and almost not expressed in other 10 tissues. Several microRNA target sites were predicted in the CDS of BMI FAR1 mRNA for further studying this gene in the future. The 3D structure of the FAR1 by homology modeling was similar to that of pseudomonas 3-alpha-hydroxysteroid dehydrogenase (2 dkn Chain: A). The experiment will establish a foundation for further insight into this swine gene. © Medwell Journals, 2012.


Wang S.Y.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Wang S.Y.,Yunnan Agricultural University | Huo J.L.,Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province | Huo J.L.,Yunnan Agricultural University | And 5 more authors.
Genetics and Molecular Research | Year: 2013

U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an important gene for pre-messenger RNA splicing in higher eukaryotes. In this study, the Banna mini-pig inbred line (BMI) U2AF2 coding sequence (CDS) was cloned, sequenced, and characterized. The U2AF2 complete CDS was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) technique based on the conserved sequence information of cattle and known highly homologous swine expressed sequence tags. This novel gene was deposited into the National Center for Biotechnology Information database (Accession No. JQ839267). Sequence analysis revealed that the BMI U2AF2 coding sequence consisted of 1416 bp and encoded 471 amino acids with a molecular weight of 53.12 kDa. The protein sequence has high sequence homology with U2AF65 of 6 species - Homo sapiens (100%), Equus caballus (100%), Canis lupus (100%), Macaca mulatta (99.8%), Bos taurus (74.4%), and Mus musculus (74.4%). The phylogenetic tree analysis revealed that BMI U2AF65 has a closer genetic relationship with B. taurus U2AF65 than with U2AF65 of E. caballus, C. lupus, M. mulatta, H. sapiens, and M. musculus. RT-PCR analysis showed that BMI U2AF2 was most highly expressed in the brain; moderately expressed in the spleen, lung, muscle, and skin; and weakly expressed in the liver, kidney, and ovary. Its expression was nearly silent in the spinal cord, nerve fiber, heart, stomach, pancreas, and intestine. Three microRNA target sites were predicted in the CDS of BMI U2AF2 messenger RNA. Our results establish a foundation for further insight into this swine gene. © FUNPEC-RP.


Qin C.,Key Laboratory of Banna Mini pig Inbred Line of Yunnan Province | Qin C.,Yunnan Agricultural University | Wang P.,Key Laboratory of Banna Mini pig Inbred Line of Yunnan Province | Wang P.,Yunnan Agricultural University | And 4 more authors.
Research Journal of Biotechnology | Year: 2014

Mitofusin 1 (MFN1) and its active GTPase domains are important for the mitochondrial fusion process. Mitochondrial fusion may regulate mitochondrial morphogenesis and underlie complementation between mitochondrial genomes in mammalian cells. In the present study, the complete CDS of BMI MFN1 was obtained and characterized (GenBank accession numbers for the nucleotide and amino acid sequences are KC505156 and AGK36094 respectively). The tissue expression profile in 30 important tissues of BMI was carried out using quantitative real-time PCR method. The full-length coding region of MFN1 from BMI tissues consists of 2226 nucleotide which encodes 741 amino acids with a molecular weight of 84.18 kD and a pI of 6.05. The putative protein of MFN1, which is located in the cytoplasm (76.7%), contains two conserved domains of Ras-like-GTPase superfamily and fzo-mitofusin superfamily, three potential miRNA targets and no signal peptide. The sequence homology analysis revealed that the BMI MFN1 protein shared 97.2%, 95.4%, 94.3%, 93.8%, 91.5% and 91.0% identity with that of cattle, bat, mouse, rat, monkey and human. The phylogenetic tree analysis revealed that the BMI MFN1 gene had a closer genetic relationship with the MFN1 gene of cattle than with those of bat, mouse, rat, monkey and human. The analysis of tissue expression showed that BMI MFN1 gene was widely expressed in the tissues examined but pancreas and colon, being high in the testis, thymus, thyroid gland, lung, heart, liver, spleen, adrenal gland, lymph node, kidney, muscle, duodenum, ileum, esophagus, cerebrum, cerebellum, pituitary gland, hypothalamus, brainstem and sublingual gland, moderate in the submaxillary gland, epididymis, rectum, skin and spinal cord, low in the jejunum, cecum and stomach. These data provide a foundation for further insight into this swine gene.


Wang P.,Key Laboratory of Banna Mini pig Inbred Line of Yunnan Province | Wang P.,Yunnan Agricultural University | Wang S.,Key Laboratory of Banna Mini pig Inbred Line of Yunnan Province | Wang S.,Yunnan Agricultural University | And 4 more authors.
Research Journal of Biotechnology | Year: 2015

The objective of this study was to clone BMI sterile and fertile PGK2 and PHKG2 genes and analyze the expression patterns in the different reproduction's testis of Banna mini-pig inbred line. Using RT-PCR, PGK2 and PHKG2 cDNAs were cloned and bioinformatics analysis was performed. Using GAPDH gene as a control, the expressions of PGK2 and PHKG2 mRNA in the testis of sterile and fertile BMI boars were detected by semi-quantitative methods. The full-length coding sequence (CDS) of the BMI PGK2 consists of 1254 bp which encodes a 417 amino acid protein with molecular mass of 44.84 kD and a pI of 8.31. And the PHKG2 CDS is 1221 bp in length which encodes a 406 amino acid protein with molecular mass of 46.56 kD and a pI of 5.86. Four and eight nucleotide differences were found in the cDNAs of PGK2 and PHKG2 between BMI but only one missing nucleotide mutation at c.970 (T>A) changes aspartic acid (Asn) at p.324 in BMI fertile boars to tyrosine (Tyr) in the BMI sterile boars protein. The putative proteins of BMI PGK2 and PHKG2 both located in the cytoplasm with high reliability contained the conserved domain and active sites of protein kinase superfamily and no signal peptide. Moreover, there is a coiled-coil motif in the N terminus of BMI PHKG2. Phylogenetic analyses based on the PGK2 and PHKG2 amino acid sequences showed that BMI was grouped with cattle, sheep and horse. The RT-PCR analysis showed that the expression of PGK2 and PHKG2 was similar in the testes of fertile and sterile BMI boar. These data provide the primary foundation for further insights into the BMI PGK2 and PHKG2 genes.


PubMed | Key Laboratory of Banna Mini Pig Inbred Line of Yunnan Province
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2013

U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an important gene for pre-messenger RNA splicing in higher eukaryotes. In this study, the Banna mini-pig inbred line (BMI) U2AF2 coding sequence (CDS) was cloned, sequenced, and characterized. The U2AF2 complete CDS was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) technique based on the conserved sequence information of cattle and known highly homologous swine expressed sequence tags. This novel gene was deposited into the National Center for Biotechnology Information database (Accession No. JQ839267). Sequence analysis revealed that the BMI U2AF2 coding sequence consisted of 1416 bp and encoded 471 amino acids with a molecular weight of 53.12 kDa. The protein sequence has high sequence homology with U2AF65 of 6 species - Homo sapiens (100%), Equus caballus (100%), Canis lupus (100%), Macaca mulatta (99.8%), Bos taurus (74.4%), and Mus musculus (74.4%). The phylogenetic tree analysis revealed that BMI U2AF65 has a closer genetic relationship with B. taurus U2AF65 than with U2AF65 of E. caballus, C. lupus, M. mulatta, H. sapiens, and M. musculus. RT-PCR analysis showed that BMI U2AF2 was most highly expressed in the brain; moderately expressed in the spleen, lung, muscle, and skin; and weakly expressed in the liver, kidney, and ovary. Its expression was nearly silent in the spinal cord, nerve fiber, heart, stomach, pancreas, and intestine. Three microRNA target sites were predicted in the CDS of BMI U2AF2 messenger RNA. Our results establish a foundation for further insight into this swine gene.

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