Key Laboratory of Artificial Cells

Tianjin, China

Key Laboratory of Artificial Cells

Tianjin, China
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Li J.-Y.,Tianjin Medical University | Li T.,The Third Central Hospital of Tianjin | Yu M.-L.,Key Laboratory of Artificial Cells | Gao W.-Q.,The Third Central Hospital of Tianjin | And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: At present, a heparin-coated extracorporeal circulation pipe is used widely, but the price is expensive and limits its wide application. Objective: To screen the optimal coating condition of oxidated sodium alginate as a coating material to coat the medical polyvinyl chloride pipe and to evaluate the anticoagulant properties and the stability of the coated pipe. Methods: Oxidized sodium alginate was prepared with sodium periodate, and the mole ratio of sodium periodate and sodium alginate was 1:8, 1:10 and 1:12, respectively. Oxidized sodium alginate with different degree of oxidation was coated on the surface of medical polyvinyl chloride pipes by chemical methods, then to select the best degree of oxidation. The optimal coating condition was screened through an orthogonal experiment based on concentrations of sulfuric acid, polyethyleneimine and oxidized sodium alginate, and pH value and temperature of oxidized sodium alginate. Additionally, the anticoagulant properties and expulsion rates of the prepared pipes were evaluated in comparison with the blank control group and heparin-coated group. Results and Conclusion: The optimal oxidation degree for oxidized sodium alginate was 1:10 for the mole ratio of sodium periodate and sodium alginate. The best coating conditions were 50% concentrated sulfuric acid, 0.05% polyethyleneimine, 40 °C of reaction temperature, 2 g/L oxidized sodium alginate, and pH value=3.5. The oxidized sodium alginate coating group had a similar trend compared with heparin-coated group in the expulsion rate. The anticoagulant properties of oxidized sodium alginate coating group were little weaker than those of heparin-coated group (P < 0.05), but significantly better than those of the blank control group. The oxidized sodium alginate-coated extracorporeal circulation pipe has a good anticoagulant property and stability.


Yu G.-D.,Tianjin Medical University | Li T.,Third Center Hospital of Tianjin | Gao W.-Q.,Third Center Hospital of Tianjin | Yu M.-L.,Key Laboratory of Artificial Cells | And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Dexamethasone as coating is fixed to the surface of cardiopulmonary bypass pipe, which can maintain sustainable anti-inflammatory effects and avoid adverse reactions due to excessive blood concentration, thereby maximizing the anti-inflammatory action of dexamethasone. OBJECTIVE: To develop a new coating method of dexamethasone sodium phosphate, which has anticoagulation activity, and to evaluate the performance of the coated pipeline, including the stability, anticoagulant and antiplatelet activity. METHODS: The surface of polyvinyl chloride pipelines in the extracorporeal circulation was successively pretreated with strong sulfuric acid and polyethylene imines. Dexamethasone sodium phosphate coated pipelines were made through two methods: ionic blond and premix. Then, a quantitative analysis was performed to evaluate anticoagulation, antiplatelet, resistance of protein adhesion and antithrombosis function. Non-coated polyvinyl chloride pipeline served as control group. RESULTS AND CONCLUSION: The biggest drug loadings were (2.06 ±0.68) and (3.33±0.75) μg/cm2 for dexamethasone sodium phosphate coated polyvinyl chloride pipelines prepared by premix and ionic blond, respectively. In the anticoagulation, antiplatelet, and resistance of protein adhesion experiment, dexamethasone sodium phosphate coated polyvinyl chloride pipelines prepared by premix were superior to those prepared by ionic blond and control group (P < 0.05). Release in vitro experiment showed that dexamethasone sodium phosphate coated polyvinyl chloride pipelines prepared by premix were also superior to those prepared by ionic blond. The findings indicate that the dexamethasone sodium phosphate coating prepared by premix shows better release and anticoagulation performance, as well as forms antiplatelet adhesion and antithrombosis function, to meet the short-term extracorporeal circulation requirements.


Gao W.-Q.,Third Central Hospital | Gao W.-Q.,Tianjin Medical University | Li T.,Third Central Hospital | Yu M.-L.,Key Laboratory of Artificial Cells | And 5 more authors.
Chinese Journal of Biomedical Engineering | Year: 2013

The aim of this work is to prepare a new composite coating based on sodium alginate and heparin with aldehyde groups, and to evaluate the biocompatibility and hemocompatibility of extracorporeal circulation loop. The loop samples were divided into four groups: (1) blank control (C), (2) sodium alginate (OSA), (3) heparin (LMNH), and (4) sodium alginate plus heparin (OSA. LMNH). The OSA and LMNH were bound to the surface of extracorporeal circulation loop by covalent linkage. Qualitative and quantitative analysis of polysaccharide fragments were conducted with FTIR and UV spectroscopy, the hemocompatibility of coated loop was evaluated by platelet adhesion, coagulation examination and protein adhesion, the hydrophily of coated loop was evaluated by surface contact angle. Experimental results showed that the surface of extracorporeal circulation loop was coated with OSA and LMNH. The platelet adhesion and thrombosis for the coated group were both decreased significantly compared with the non-coated group (P < 0.05). The protein adhesion of LMNH group has no significantly difference with the non-coated group. The protein adhesion of OSA group and OSA. LMNH group significantly decreased compared with LMNH group (P < 0.05). The anticoagulant property of LMNH group and OSA. LMNH group is good with significantly prolonged time of APTT and TT. The APTT time of OSA group is significantly prolonged compared with C group (p < 0.05). The surface contact angles of OSA and OSA. LMNH group is significantly decreased compared with C group(p < 0.05). The surface contact angle of LMNH group has no significantly difference with C group. Composite coating of polysaccharide fragments has improved anticoagulant property in reference to the control, and the hemocompatibility of the composite coating was obviously improved compared with single coating.


Li J.-Y.,Tianjin Medical University | Li T.,Third Central Hospital | Gao W.-Q.,Third Central Hospital | Yu M.-L.,Key Laboratory of Artificial Cells | And 4 more authors.
Chinese Journal of Biomedical Engineering | Year: 2012

This study is aimed to use oxidated sodium alginate(OSA) to coat medical polyvinyl chloride (PVC) pipe used for extracorporeal circulation, and evaluate the hemocompatibility and biocompatibility. The PVC pipe was coated with OSA. Its hemocompatibility and biocompatibility were evaluated and compared with those of PVCC and heparin-coated PVCM. The anticoagulant properties of oxidated sodium alginate coating group (PVCS) was lower than PVCM (P < 0.05), but significantly better than that of PVCC (P < 0.05). The protein adhesion of PVCS was significantly lower than that of the PVCC (P < 0.05) and PVCM (P < 0.05). The platelet adhesion of PVCS was significantly lower than that of PVCC (P < 0.05), but had no significantly difference from that of PVCM (P > 0.05). The expulsion rates of PVCS had a similar tendency to that of PVCM in 72 hours. CONCLUSION: The PVCS coating improved biocompatibility, anticoagulant property and stability of PVC.


Lu H.-B.,Tianjin Medical University | Li T.,Third Central Hospital | Duan D.-W.,Third Central Hospital | Gao W.-Q.,Third Central Hospital | And 4 more authors.
Chinese Journal of Biomedical Engineering | Year: 2012

In order to develop novel aterial filter with excellent biocompatibility, we prepared dacron filter coated with low molecular weight heparin (LMWH) or partial oxidation sodium alginate (OSA). The functional groups of the surface coating were determined with infrared spectroscopy. The biocompatibility was evaluated including contact angle, protein adhesion, in vitro platelet adhesion, coagulation indices, in vitro thrombosis. The safty was assessed by the loss of coating material, flow/roating speed, inlet and trans-filter pressure during analog circulation. There are similar changes in the infrared spectroscopy of coated material at the location of LMWH's or OSA's absorption peak. After being coated, the contact angle, albumin and fibrinogen adhering to the surface of dacron filter were decreased (P < 0.05). For the LMWH coating, contact angle (32.7 ± 4.65)°, albumin and fibrinogen adhered (2.56 ± 0.81,1.63 ± 0.37) μ,g/cm2 were higher than that (25.2 ± 4.02)°, (2.00 ± 0.32, 1.32 ± 0.14) μg/cm 2 of OSA coating. The anticoagulant activity was notably promoted by coating, and dacron coated with LMWH was better than OSA (P < 0.05). There was about 10% coating material loss in the analog circulation. The inlet and trans-filter pressure of coating filters was slightly increased, and flow/rotate was decreased. In conclusion, chemical graft-modification can be applied to the dacron filter. The biocompatibility of the coated materials is significantly promoted, and the safty meet the clinical demand. LMWH coating material has better hemocompatibility, while OSA coating material have better histocompatibility. For the carrier ability and low cost, OSA coating would get more development space.

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