Time filter

Source Type

Wang Y.,Key Laboratory of Aquatic Product Processing | Li Z.,Kemin Industries Zhuhai Co. | Li J.,Chinese Academy of Fishery Sciences | Duan Y.-F.,Key Laboratory of Aquatic Product Processing | And 4 more authors.
Fish and Shellfish Immunology | Year: 2015

An eight-week feeding trial followed by an acute combined stress test of low-salinity and nitrite were performed to evaluate effects of chlorogenic acid (CGA) on growth performance and antioxidant capacity of white shrimp Litopenaeus vannamei. Shrimp were randomly allocated in 12 tanks (30 shrimp per tank) and triplicate tanks were fed with a control diet or diets containing different levels of CGA (100, 200 and 400mgkg-1 feed) as treatment groups. Growth performance including weight gain (WG), biomass gain (BG), feed conversion ratio (FCR), and feed intake were determined after feeding for 56 days. Antioxidant capacity were evaluated by determining the activity of total antioxidant status (TAS), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) as well as the gene expression of GSH-Px and CAT in the hepatopancreas of shrimp at the end of feeding trial and again at the end of the combined stress test. The results indicated that supplemention of CGA had no significant effects on the growth performance and the activities of TAS, SOD, GSH-Px and CAT in hepatopancreas of shrimp cultured under normal conditions for 56 days. However, compared with the control group, CGA (200, 400mgkg-1 feed) significantly improved the resistance of L.vannamei against the combined stress of low-salinity and nitrite, as indicated by the significant (P<0.05) higher survival, higher activities of TAS, GSH-Px and CAT, as well as higher transcript levels of GPx and CAT gene in shrimp treated with CGA in the combined tress test. Our findings suggested that CGA possessed dual-modulatory effects on antioxidant capacity of L.vannamei and could be a potential feed additive that can enhance shrimp resistance against environmental stresses. The recommended application dosage is 200mgkg-1 and further studies are needed to clarify the action model of CGA efficiency. © 2015 Elsevier Ltd.


Wang Z.,CAS South China Sea Fisheries Research Institute | Wang Z.,Key Laboratory of Fishery Ecology and Environment | Wang Z.,Key Laboratory of Aquatic Product Processing | Wang X.,CAS South China Sea Fisheries Research Institute | And 3 more authors.
Journal of Applied Phycology | Year: 2014

The macroalga Gracilaria lemaneiformis is an important and commercially valuable renewable resource. It is distributed widely in shallow marine waters but grows mostly on tropical or subtropical coasts. We investigated the accumulation of Cd, Cu, and Pb by live G. lemaneiformis under low concentrations. There was a positive correlation between the organisms' metal concentrations and exposure concentrations. When exposed to both Cu and Cd, the concentrations of Cu and Cd in G. lemaneiformis were higher than those exposed to solutions of Cu and Cd alone. However, the concentrations of heavy metals in G. lemaneiformis were not markedly different (p < 0.05) between the treatment groups and the control groups. We analyzed the results with nonlinear curve fitting and employed a two-compartment model to study the accumulation kinetics of heavy metals by G. lemaneiformis. The uptake rate constants and bioconcentration factors (BCFs) of the metals decreased with increased exposure concentration. The theoretical equilibrium concentrations increased significantly with the exposure concentrations. Our results suggested that G. lemaneiformis obviously accumulated heavy metals from seawater. As an important resource for food and pharmaceuticals, G. lemaneiformis should be cultivated in clear seawater. © 2013 Springer Science+Business Media Dordrecht.


Ke C.-L.,CAS South China Sea Fisheries Research Institute | Ke C.-L.,Key Laboratory of Aquatic Product Processing | Wang Z.-H.,CAS South China Sea Fisheries Research Institute | Wang Z.-H.,Key Laboratory of Aquatic Product Processing | And 10 more authors.
RSC Advances | Year: 2014

We developed a highly sensitive and specific method for determining DES residue in fish meat using gas chromatography coupled with tandem mass spectrometry (GC-MS-MS) with a triple quadrupole analyzer (QqQ). The samples were extracted using ethyl acetate, cleaned using florisil, and derivatized using a mixture of 99% N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and 1% trimethylchlorosilane (TMCS). The results showed that the GC-MS-MS system presented great advantages for the identification and quantitation of DES in fish meat. The limit of detection (LOD) and limit of quantification (LOQ) values were 0.0142 μg kg-1 and 0.0475 μg kg-1, respectively. The recoveries from the spiked fish samples spiked at four levels were in the range of 86.1% to 106%. The precision of the method was evaluated using the relative standard deviation (RSD) and ranged from 1.39% to 9.62%. The linear range was from 1.00 to 100 μg L-1 with good linearity (r = 0.9998). It can be concluded that the method is reliable for identifying and detecting DES residue at trace levels in aquatic products. © 2014 The Royal Society of Chemistry.


Wang Z.-H.,CAS South China Sea Fisheries Research Institute | Wang Z.-H.,Key Laboratory of Fishery Ecology Environment | Wang Z.-H.,Key Laboratory of Aquatic Product Processing | Wang X.-N.,CAS South China Sea Fisheries Research Institute | And 2 more authors.
Yejin Fenxi/Metallurgical Analysis | Year: 2014

An atomic absorption spectrometric method for the determination of copper, lead, cadmium, zinc, chromium and nickel in seawater was established. Under the conditions of pH 4.0±0.5 and heating in a water bath, with ammonium pyrrolidine dithiocarbamate (APDC) as a chelating agent, copper, lead, cadmium, zinc, chromium and nickel were chelated quantitatively to form chelates which were extracted by MIBK, then zinc was determined by flame atomic absorption spectrometry and the other elements were determined by graphite furnace atomic absorption spectrometry. The results showed that the detection limits for cadmium, chromium, copper, lead, nickel and zinc were 0.04, 0.3, 0.13, 0.2, 0.4 and 1.0 g/L, respectively. Certified reference seawater samples GBW (E) 080040 was analyzed, except that nickel had no certified value, the measured results of the other elements were consistent with the certified values. The method was applied to the analysis of seawater from the Pearl River estuary and the Daya Bay, with the relative standard deviations (RSD, n=7) for the measurement results of each element between 2.3% to 6.5% and the recoveries in the range of 94%-105%.


Wu Y.Y.,CAS South China Sea Fisheries Research Institute | You G.,Key Laboratory of Aquatic Product Processing | Li L.H.,Ocean University of China
Lecture Notes in Electrical Engineering | Year: 2015

Salted fish is a traditional Chinese aquatic product, because of its rich nutrition and the salty, sweet, unique taste and flavor, it is loved by consumers. At present, empirical traditional processing methods are adopted in enterprises that processing salted aquatic products, which have many disadvantages not only in the production scale, but also its efficiency. In order to innovate the traditional salted fish processing technology, as well as to meet the demand of modern consumers for salted fish with lower salt, richer nutrition, higher safety, better quality and flavor, this paper reviews researches on the isolation of dominant microorganisms in traditional salted fish and the application of lactic acid bacteria and other microorganisms in the salted fish processing, further, puts forward the tendency of applying modern microbial technologies to the processing of salted fish products. © Springer-Verlag Berlin Heidelberg 2015.


Lin W.-L.,Key Laboratory of Aquatic Product Processing | Yang X.-Q.,Key Laboratory of Aquatic Product Processing | Hou C.-L.,Key Laboratory of Aquatic Product Processing | Hao S.-X.,Key Laboratory of Aquatic Product Processing | And 6 more authors.
Modern Food Science and Technology | Year: 2013

During immersion chilling and freezing (ICF) process, mass transfer was a major factor directly affecting the application of ICF. The penetration model and law of different solutes during ICF was investigated by establishing the gelatin models and explored the penetration law of shrimp. The results indicated that the penetration depths of betaine, propanediol and sodium chloride in gelatin model were 0.2 cm, 0.35 cm and 0.4 cm, respectively, while the penetration depths of them in shrimps were 0.25 cm, 0.31 cm and 0.35 cm, respectively. The transfer contents of betaine, propanediol and sodium chloride in gelatin were 4.35±0.12 mg/g, 6.15±0.12 mg/g and 11.73±0.18 mg/g, respectively, and the transfer contents of them in shrimps were 0.86±0.07 mg/g, 1.53±0.11 mg/g and 1.67±0.08 mg/g, respectively. Through regression analysis of solute changes during ICF, the solute penetration regression model could express the penetration law of solute. It was found that transfer trend of betaine, propanediol and sodium chloride in shrimp and gelatin were consistent. Before 5 min, the penetration rates of betaine, propanediol and sodium chloride showed a linear increase. After 6 min, the penetration rates became slow. According to the math model, the diffusion coefficients of betaine, propanediol and sodium chloride in gelatin model were 4.16×10-7 m2/s, 1.27×10-6 m2/s and 1.66×10-6 m2/s, respectively. The diffusion coefficients of three solutes in shrimps were 2.34×10-7 m2/s, 9.36×10-7 m2/s and 1.36×10-6 m2/s, respectively.


Ke C.-L.,Key Laboratory of Aquatic Product Processing | Ke C.-L.,CAS South China Sea Fisheries Research Institute | Gan J.-L.,Key Laboratory of Aquatic Product Processing | Gan J.-L.,CAS South China Sea Fisheries Research Institute | And 10 more authors.
Fresenius Environmental Bulletin | Year: 2012

The sorption behavior of endosulfan onto aquaculture pond sediment was determined by using batch experiments. Furthermore, the influence of pH, ionic strength and temperature was investigated. The adsorption equilibrium was established within 4 h, and adsorption process was divided into a rapid and a slow process before and after the equilibrium time, respectively. The Freundlich isotherm model fits the adsorption data with R2>0.99 in all cases. The sorption increased at pH 5.8 (Kf= 6.62 and n = 1.05) but decreased at pH 8.0 (Kf = 3.19 and n = 0.79). Ionic strength has effects on the sorption; the Kf was two times higher in 35 g/L NaCl solution as a result of salting-out effect. Though lower temperature (20 °C., Kf = 7.09 and n = 1.02) was more favorable for adsorption, the effect was not significant. Pond is a relatively close environment, and the transport, transformation and fate of endosulfan mainly occurred in the internal circle. The results may be help to minimize its ecological risk in ponds with proper measures taken to adjust the conditions of pH, temperature and ionic strength.


Ke C.,CAS South China Sea Fisheries Research Institute | Ke C.,Key Laboratory of Aquatic Product Processing | Liu Q.,CAS South China Sea Fisheries Research Institute | Liu Q.,Key Laboratory of Aquatic Product Processing | And 8 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2016

Gas chromatography (GC) coupled with triple quadrupole tandem mass spectrometry (MS/MS) operated in electron ionization mode (EI) has been shown to have advantages in the trace analysis of chemical compounds. Employing the instrument, a method has been built to simultaneously determine eugenol, isoeugenol‚ and methyleugenol, which have been widely used as fish anesthetic, in the fish fillet. Procedure for the sample preparation was achieved by using hexane extraction followed by phenyl solid phase extraction (SPE) cleanup, which was free of such steps as rotary evaporation and nitrogen blowing by taking the volatility of eugenol and its isomers into consideration. The method was validated by conducting recovery studies on fortified fish fillet samples at four concentrations. The linearity in the range of 5–500 μg·L−1 was forced through the origin giving a coefficient of determination (r2) greater than 0.9982. Limits of detection (LODs) for eugenol, isoeugenol‚ and methyleugenol were 0.4, 1.2‚ and 0.2 μg·kg−1, respectively. The limits of quantification (LOQs) were 1.2, 4‚ and 0.7 μg·kg−1 for eugenol, isoeugenol‚ and methyleugenol, respectively. The recoveries for eugenol and its isomers ranged from 76.4 to 99.9% with relative standard deviations (RSD) in a range from 2.18 to 15.5%. This method is quick, simple and suitable for determining the residues of eugenol, isoeugenol and methyleugenol simultaneously in batch samples of fish fillet. © 2016 Elsevier B.V.

Loading Key Laboratory of Aquatic Product Processing collaborators
Loading Key Laboratory of Aquatic Product Processing collaborators