Key Laboratory of Aquatic Product Processing

Guangzhou, China

Key Laboratory of Aquatic Product Processing

Guangzhou, China
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Ke C.-L.,CAS South China Sea Fisheries Research Institute | Ke C.-L.,Key Laboratory of Aquatic Product Processing | Gu Y.-G.,CAS South China Sea Fisheries Research Institute | Gu Y.-G.,Guangdong Provincial Key Laboratory of Fishery Ecology and Environment | And 11 more authors.
Marine Pollution Bulletin | Year: 2017

Concentrations of 16 US EPA priority polycyclic aromatic hydrocarbons (PAHs) were measured in 15 marine wild organism species from South China Sea. The concentration (dry weight) of 16 PAHs ranged from 94.88 to 557.87ng/g, with a mean of 289.86ng/g. The concentrations of BaP in marine species were no detectable. The composition of PAHs was characterized by the 2- and 3-ring PAHs in marine species, and NA, PHE and FA were the dominant constituents. PAHs isomeric ratios indicated PAHs mainly originated from grass, wood and coal combustion, and petroleum. The human health risk assessment based on the excess cancer risk (ECR) suggested the probability of PAHs posing carcinogenic risk to human beings with consumption of marine organisms were negligible (probability<1×10-6). © 2017.


Wang Y.,Key Laboratory of Aquatic Product Processing | Li J.,Key Laboratory for Sustainable Development of Marine Fisheries | He Y.,Key Laboratory for Sustainable Development of Marine Fisheries | Duan Y.,Key Laboratory of Aquatic Product Processing | Zhang Z.,Key Laboratory of Aquatic Product Processing
Journal of Fishery Sciences of China | Year: 2017

Chinese shrimp, Fenneropenaeus chinensis, is widely cultured in China for its high economic value. However, farming yields of F. chinensis have decreased in the past few decades. The decreases in yield has been attributed to disease, as environmental deterioration due to poor management has resulted in an increased incidence of stress-induced disease. Environmental stressors, such as ammonia, have a significant effect on the antioxidant system of shrimp. However, little information is available about the effects of changes in ambient ammonia-N on hemolymph nitrogen content and antioxidant capability of Chinese shrimp. Therefore, 600 shrimps [initial body weight, (5.0±1.2) g] were divided randomly into five groups and exposed to water containing different concentrations of ambient ammonia-N (0 mg/L, 2 mg/L, 4 mg/L, 6 mg/L, and 8 mg/L). Each group had six replicates of 20 shrimp each. Total antioxidant capacity (T-AOC), antisuperoxide anions, relative expression of catalase (CAT), peroxiredoxin (Prx), and caspase mRNA in hemocytes and hemolymph ammonia, and urea content were analyzed in hemolymph after exposing shrimp to different concentrations of ambient ammonia-N for 6 h, 24 h, 48 h, 72 h, and 96 h. Shrimp cultured in filtered seawater were used as the control. The results showed that ammonia accumulated gradually in the hemolymph as exposure time increased. Hemolymph ammonia content of shrimp in the 8 mg/L group was the highest compared with that in the other groups and was 5.85 times higher than that in the control group. Hemolymph urea contents of shrimp in the ammonia-N exposed groups were higher than that in the control group after 6 h of ammonia-N exposure. The hemolymph urea of shrimp in the 6 mg/L group was the highest after 6 h and was 2.22-times higher than that in the control group. Hemolymph T-AOC and Prx mRNA expression in hemocytes of shrimp exposed to ambient ammonia-N increased initially and then decreased as exposure time increased. However, anti-superoxide anions in hemolymph and relative CAT and caspase mRNA expression levels in hemocytes increased, decreased, and then increased again as exposure time increased. The antioxidant capacity of shrimp was significantly higher after exposure to ammonia-N than that in the control. These findings demonstrate that the oxidation-antioxidant balance system in shrimp may be damaged after exposure to ammonia-N, resulting in upregulation of caspase mRNA expression.


Ke C.,CAS South China Sea Fisheries Research Institute | Ke C.,Key Laboratory of Aquatic Product Processing | Liu Q.,CAS South China Sea Fisheries Research Institute | Liu Q.,Key Laboratory of Aquatic Product Processing | And 11 more authors.
Aquaculture | Year: 2017

Levels of eugenol, isoeugenol and methyleugenol were investigated in 216 seawater fish and 321 freshwater fish from wholesale markets. Eugenol was detected in 55 samples with concentrations ranging from 3.11 to 30,690. μg/kg. Isoeugenol was detected only in two samples of grouper at concentrations of 1032. μg/kg and 86.2. μg/kg. Methyleugenol was not detected in any samples. The incidence of anesthetic residue in all the samples was 10.6%. The frequencies of species found with anesthetic residue were higher in seawater fish than in freshwater fish, with rates of 19.4% and 4.7%, respectively. The warm seawater fish such as grouper, sea bass, sea bream, pompano, and red drum should be monitored more closely in the future due to the higher detection frequencies and values of these compounds. Although the results indicate that eugenol or isoeugenol have been used as fish anesthetic to assist with fish handling and fish transport in some species, the human health risk is negligible. © 2017 Elsevier B.V.


Wang Y.,Key Laboratory of Aquatic Product Processing | Li Z.,Kemin Industries Zhuhai Co. | Li J.,Chinese Academy of Fishery Sciences | Duan Y.-F.,Key Laboratory of Aquatic Product Processing | And 4 more authors.
Fish and Shellfish Immunology | Year: 2015

An eight-week feeding trial followed by an acute combined stress test of low-salinity and nitrite were performed to evaluate effects of chlorogenic acid (CGA) on growth performance and antioxidant capacity of white shrimp Litopenaeus vannamei. Shrimp were randomly allocated in 12 tanks (30 shrimp per tank) and triplicate tanks were fed with a control diet or diets containing different levels of CGA (100, 200 and 400mgkg-1 feed) as treatment groups. Growth performance including weight gain (WG), biomass gain (BG), feed conversion ratio (FCR), and feed intake were determined after feeding for 56 days. Antioxidant capacity were evaluated by determining the activity of total antioxidant status (TAS), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) as well as the gene expression of GSH-Px and CAT in the hepatopancreas of shrimp at the end of feeding trial and again at the end of the combined stress test. The results indicated that supplemention of CGA had no significant effects on the growth performance and the activities of TAS, SOD, GSH-Px and CAT in hepatopancreas of shrimp cultured under normal conditions for 56 days. However, compared with the control group, CGA (200, 400mgkg-1 feed) significantly improved the resistance of L.vannamei against the combined stress of low-salinity and nitrite, as indicated by the significant (P<0.05) higher survival, higher activities of TAS, GSH-Px and CAT, as well as higher transcript levels of GPx and CAT gene in shrimp treated with CGA in the combined tress test. Our findings suggested that CGA possessed dual-modulatory effects on antioxidant capacity of L.vannamei and could be a potential feed additive that can enhance shrimp resistance against environmental stresses. The recommended application dosage is 200mgkg-1 and further studies are needed to clarify the action model of CGA efficiency. © 2015 Elsevier Ltd.


Wang Z.,CAS South China Sea Fisheries Research Institute | Wang Z.,Key Laboratory of Fishery Ecology and Environment | Wang Z.,Key Laboratory of Aquatic Product Processing | Wang X.,CAS South China Sea Fisheries Research Institute | And 3 more authors.
Journal of Applied Phycology | Year: 2014

The macroalga Gracilaria lemaneiformis is an important and commercially valuable renewable resource. It is distributed widely in shallow marine waters but grows mostly on tropical or subtropical coasts. We investigated the accumulation of Cd, Cu, and Pb by live G. lemaneiformis under low concentrations. There was a positive correlation between the organisms' metal concentrations and exposure concentrations. When exposed to both Cu and Cd, the concentrations of Cu and Cd in G. lemaneiformis were higher than those exposed to solutions of Cu and Cd alone. However, the concentrations of heavy metals in G. lemaneiformis were not markedly different (p < 0.05) between the treatment groups and the control groups. We analyzed the results with nonlinear curve fitting and employed a two-compartment model to study the accumulation kinetics of heavy metals by G. lemaneiformis. The uptake rate constants and bioconcentration factors (BCFs) of the metals decreased with increased exposure concentration. The theoretical equilibrium concentrations increased significantly with the exposure concentrations. Our results suggested that G. lemaneiformis obviously accumulated heavy metals from seawater. As an important resource for food and pharmaceuticals, G. lemaneiformis should be cultivated in clear seawater. © 2013 Springer Science+Business Media Dordrecht.


Ke C.-L.,CAS South China Sea Fisheries Research Institute | Ke C.-L.,Key Laboratory of Aquatic Product Processing | Wang Z.-H.,CAS South China Sea Fisheries Research Institute | Wang Z.-H.,Key Laboratory of Aquatic Product Processing | And 10 more authors.
RSC Advances | Year: 2014

We developed a highly sensitive and specific method for determining DES residue in fish meat using gas chromatography coupled with tandem mass spectrometry (GC-MS-MS) with a triple quadrupole analyzer (QqQ). The samples were extracted using ethyl acetate, cleaned using florisil, and derivatized using a mixture of 99% N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and 1% trimethylchlorosilane (TMCS). The results showed that the GC-MS-MS system presented great advantages for the identification and quantitation of DES in fish meat. The limit of detection (LOD) and limit of quantification (LOQ) values were 0.0142 μg kg-1 and 0.0475 μg kg-1, respectively. The recoveries from the spiked fish samples spiked at four levels were in the range of 86.1% to 106%. The precision of the method was evaluated using the relative standard deviation (RSD) and ranged from 1.39% to 9.62%. The linear range was from 1.00 to 100 μg L-1 with good linearity (r = 0.9998). It can be concluded that the method is reliable for identifying and detecting DES residue at trace levels in aquatic products. © 2014 The Royal Society of Chemistry.


Wang Z.-H.,CAS South China Sea Fisheries Research Institute | Wang Z.-H.,Key Laboratory of Fishery Ecology Environment | Wang Z.-H.,Key Laboratory of Aquatic Product Processing | Wang X.-N.,CAS South China Sea Fisheries Research Institute | And 2 more authors.
Yejin Fenxi/Metallurgical Analysis | Year: 2014

An atomic absorption spectrometric method for the determination of copper, lead, cadmium, zinc, chromium and nickel in seawater was established. Under the conditions of pH 4.0±0.5 and heating in a water bath, with ammonium pyrrolidine dithiocarbamate (APDC) as a chelating agent, copper, lead, cadmium, zinc, chromium and nickel were chelated quantitatively to form chelates which were extracted by MIBK, then zinc was determined by flame atomic absorption spectrometry and the other elements were determined by graphite furnace atomic absorption spectrometry. The results showed that the detection limits for cadmium, chromium, copper, lead, nickel and zinc were 0.04, 0.3, 0.13, 0.2, 0.4 and 1.0 g/L, respectively. Certified reference seawater samples GBW (E) 080040 was analyzed, except that nickel had no certified value, the measured results of the other elements were consistent with the certified values. The method was applied to the analysis of seawater from the Pearl River estuary and the Daya Bay, with the relative standard deviations (RSD, n=7) for the measurement results of each element between 2.3% to 6.5% and the recoveries in the range of 94%-105%.


Wu Y.Y.,CAS South China Sea Fisheries Research Institute | You G.,Key Laboratory of Aquatic Product Processing | Li L.H.,Ocean University of China
Lecture Notes in Electrical Engineering | Year: 2015

Salted fish is a traditional Chinese aquatic product, because of its rich nutrition and the salty, sweet, unique taste and flavor, it is loved by consumers. At present, empirical traditional processing methods are adopted in enterprises that processing salted aquatic products, which have many disadvantages not only in the production scale, but also its efficiency. In order to innovate the traditional salted fish processing technology, as well as to meet the demand of modern consumers for salted fish with lower salt, richer nutrition, higher safety, better quality and flavor, this paper reviews researches on the isolation of dominant microorganisms in traditional salted fish and the application of lactic acid bacteria and other microorganisms in the salted fish processing, further, puts forward the tendency of applying modern microbial technologies to the processing of salted fish products. © Springer-Verlag Berlin Heidelberg 2015.


Lin W.-L.,Key Laboratory of Aquatic Product Processing | Yang X.-Q.,Key Laboratory of Aquatic Product Processing | Hou C.-L.,Key Laboratory of Aquatic Product Processing | Hao S.-X.,Key Laboratory of Aquatic Product Processing | And 6 more authors.
Modern Food Science and Technology | Year: 2013

During immersion chilling and freezing (ICF) process, mass transfer was a major factor directly affecting the application of ICF. The penetration model and law of different solutes during ICF was investigated by establishing the gelatin models and explored the penetration law of shrimp. The results indicated that the penetration depths of betaine, propanediol and sodium chloride in gelatin model were 0.2 cm, 0.35 cm and 0.4 cm, respectively, while the penetration depths of them in shrimps were 0.25 cm, 0.31 cm and 0.35 cm, respectively. The transfer contents of betaine, propanediol and sodium chloride in gelatin were 4.35±0.12 mg/g, 6.15±0.12 mg/g and 11.73±0.18 mg/g, respectively, and the transfer contents of them in shrimps were 0.86±0.07 mg/g, 1.53±0.11 mg/g and 1.67±0.08 mg/g, respectively. Through regression analysis of solute changes during ICF, the solute penetration regression model could express the penetration law of solute. It was found that transfer trend of betaine, propanediol and sodium chloride in shrimp and gelatin were consistent. Before 5 min, the penetration rates of betaine, propanediol and sodium chloride showed a linear increase. After 6 min, the penetration rates became slow. According to the math model, the diffusion coefficients of betaine, propanediol and sodium chloride in gelatin model were 4.16×10-7 m2/s, 1.27×10-6 m2/s and 1.66×10-6 m2/s, respectively. The diffusion coefficients of three solutes in shrimps were 2.34×10-7 m2/s, 9.36×10-7 m2/s and 1.36×10-6 m2/s, respectively.


Ke C.,CAS South China Sea Fisheries Research Institute | Ke C.,Key Laboratory of Aquatic Product Processing | Liu Q.,CAS South China Sea Fisheries Research Institute | Liu Q.,Key Laboratory of Aquatic Product Processing | And 8 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2016

Gas chromatography (GC) coupled with triple quadrupole tandem mass spectrometry (MS/MS) operated in electron ionization mode (EI) has been shown to have advantages in the trace analysis of chemical compounds. Employing the instrument, a method has been built to simultaneously determine eugenol, isoeugenol‚ and methyleugenol, which have been widely used as fish anesthetic, in the fish fillet. Procedure for the sample preparation was achieved by using hexane extraction followed by phenyl solid phase extraction (SPE) cleanup, which was free of such steps as rotary evaporation and nitrogen blowing by taking the volatility of eugenol and its isomers into consideration. The method was validated by conducting recovery studies on fortified fish fillet samples at four concentrations. The linearity in the range of 5–500 μg·L−1 was forced through the origin giving a coefficient of determination (r2) greater than 0.9982. Limits of detection (LODs) for eugenol, isoeugenol‚ and methyleugenol were 0.4, 1.2‚ and 0.2 μg·kg−1, respectively. The limits of quantification (LOQs) were 1.2, 4‚ and 0.7 μg·kg−1 for eugenol, isoeugenol‚ and methyleugenol, respectively. The recoveries for eugenol and its isomers ranged from 76.4 to 99.9% with relative standard deviations (RSD) in a range from 2.18 to 15.5%. This method is quick, simple and suitable for determining the residues of eugenol, isoeugenol and methyleugenol simultaneously in batch samples of fish fillet. © 2016 Elsevier B.V.

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