Entity

Time filter

Source Type


Luo Q.,Key Laboratory of Applied Marine Biotechnology | Zhu Z.,Key Laboratory of Applied Marine Biotechnology | Yang R.,Key Laboratory of Applied Marine Biotechnology | Qian F.,Key Laboratory of Applied Marine Biotechnology | And 2 more authors.
PLoS ONE | Year: 2014

Pyropia has a unique heteromorphic life cycle with alternation stages between thallus and conchocelis, which lives at different water temperatures in different seasons. To better understand the different adaptation strategies for temperature stress, we tried to observe comparative biochemical changes of Pyropia haitanensis based on a short term heat shock model. The results showed that: (1) At normal temperature, free-living conchocelis contains significantly higher levels of H2O2, fatty acid-derived volatiles, the copy number of Phrboh and Phhsp70 genes,the activities of NADPH oxidase and floridoside than those in thallus. The released H2O2 and NADPH oxidase activity of conchocelis were more than 7 times higher than those of thallus. The copy number of Phrboh in conchocelis was 32 times that in thallus. (2) After experiencing heat shock at 35°C for 30 min, the H 2O2 contents, the mRNA levels of Phrboh and Phhsp70, NADPH oxidase activity and the floridoside content in thallus were all significantly increased. The mRNA levels of Phrboh increased 5.78 times in 5 min, NADPH oxidase activity increased 8.45 times in 20 min. (3) Whereas, in conchocelis, the changes in fatty acids and their down-stream volatiles predominated, significantly increasing levels of saturated fatty acids and decreasing levels of polyunsaturated fatty acids occurred, and the 8-carbon volatiles were accumulated. However, the changes in H2O2 content and expression of oxidant-related genes and enzymatic activity were not obvious. Overall, these results indicate that conchocelis maintains a high level of active protective apparatus to endure its survival at high temperature, while thallus exhibit typical stress responses to heat shock. It is concluded that Pyropia haitanensis has evolved a delicate strategy for temperature adaptation for its heteromorphic life cycle. © 2014 Luo et al. Source


Yang T.,National University of Singapore | Chen X.,National University of Singapore | Jin H.-X.,Key Laboratory of Applied Marine Biotechnology | Sethi G.,National University of Singapore | Go M.-L.,National University of Singapore
European Journal of Medicinal Chemistry | Year: 2015

Sirtuins are protein deacylases with regulatory roles in metabolism and stress response. Functionalized tetrahydro-1H-pyrido[4,3-b]indoles were identified as preferential sirtuin 2 inhibitors, with in vitro inhibitory potencies in the low micromolar concentrations (IC50 3-4 μM) for the more promising candidates. The functional relevance of sirtuin inhibition was corroborated in western blots that showed hyperacetylation of p53 and ±-tubulin in treated HepG2 and MDA-MB-231 cells. Molecular docking showed that the tetrahydropyridoindole scaffold was positioned in the NAD + pocket and the acetylated substrate channel of the sirtuin 2 protein by van der Waals/hydrophobic, H bonding and stacking interactions. Functionalized tetrahydropyridoindoles represent a novel class of sirtuin 2 inhibitors that could be further explored for its therapeutic potential. © 2014 Elsevier Masson SAS. Source


Weng P.F.,Key Laboratory of Applied Marine Biotechnology | Wu Z.F.,Key Laboratory of Applied Marine Biotechnology | Wu Z.F.,Ningbo University | Shen X.Q.,Key Laboratory of Applied Marine Biotechnology | Liu P.,Ningbo University
Journal of Food Process Engineering | Year: 2011

A strain of lactic acid bacteria (LAB) Lact.8 was applied in low-salinity pickled processing of mustard tuber, using this LAB as fermentation starter in order to control harmful microbes. Meanwhile, a cleaner production technique with no saline wastewater was set up in pickled processing by using LAB technology. In this study, two kinds of production technique were compared, which was low-salinity concentration pickling inoculated with Lact.8 and a traditional high-salinity pickling method. The strain Lact.8 was identified as Lactobacillus plantarum by using 16S rRNA gene sequence assay. The end product quality of pickle mustard tuber by new technique was better than that of traditional product in flavor, texture and safety by sample evaluation method. The leaching liquor presented rich in nutrition compositions by quality analysis, and developed as condiment juice. The essential free amino acids reached 112 mg/L in the juice, accounting for 39.7% in total free amino acids. This pickled processing technique is compatible to a cleaner production technique. PRACTICAL APPLICATIONS The traditional pickled vegetables processing was well known that it causes a series of problems including the product quality and environmental damage of waste water with high salinity concentration. The experimental achievements and a new type of processing technology in this article are benefit to overcome these disadvantages via using lactic acid bacteria technology, which result in the improving of product quality, including nutrient value and sensory quality and economic efficiency at a condition of low salinity pickling. It is also called a cleaner production technology. Therefore, this processing mode was fully considered to further practical application in present vegetables prickle processing industry. © 2009 Wiley Periodicals, Inc. Source


Qian F.,Key Laboratory of Applied Marine Biotechnology | Luo Q.,Key Laboratory of Applied Marine Biotechnology | Yang R.,Key Laboratory of Applied Marine Biotechnology | Zhu Z.,Key Laboratory of Applied Marine Biotechnology | And 3 more authors.
Journal of Applied Phycology | Year: 2014

Intertidal marine algae experience various abiotic stresses during low tide, such as desiccation. In this study, a red alga, Pyropia haitanensis, which is extremely tolerant to desiccation, was selected to investigate the physiological, chemical, and molecular responses of marine algae to desiccation. Osmoregulation and the synthesis of short-chain volatile compounds were studied in detail. The results showed that desiccation induced morphological and cellular changes, as well as a loss of about 98 % of the cell water. Desiccation markedly increased the content of osmoregulator floridoside in the alga. Two genes, PhNHO1, which encodes glycerokinase, and PhGPDH, which encodes glycerol-3-phosphate dehydrogenase, are involved in the biosynthesis of a floridoside precursor, glycerol-3-phosphate. Both genes were upregulated during desiccation. The species and content of short-chain volatiles changed considerably after the exposure to desiccation-inducing conditions. These changes included the production of 5-octen-1-ol, E,E-2,4-octadien-1-ol, 1-octanol, (6Z)-nonen-1-ol, and 2-nonenal, as well as the release of significant amounts of 3-octanone, dodecanoic acid, and 1-octen-3-ol. PhLOX1 and PhLOX2, which facilitate the initiation of production of downstream short-chain volatile compounds via the oxylipin pathway, were also upregulated. In summary, when exposed to desiccation conditions during low tide, stress-related responses were trigged in the alga. The concentration of floridoside, a solute involved in the osmoregulation and the expression of genes responsible for its synthesis, was increased to protect the cell from dehydration damage. Short-chain volatiles may act as pheromones and antibacterial agents. © 2014, Springer Science+Business Media Dordrecht. Source

Discover hidden collaborations