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Wang G.,State Key Laboratory of Veterinary Etiological Biology | Wang G.,Chinese Academy of Agricultural Sciences | Wang Y.,State Key Laboratory of Veterinary Etiological Biology | Wang Y.,Chinese Academy of Agricultural Sciences | And 6 more authors.
Virology Journal | Year: 2015

This study reviews the FMDV receptor-binding domain, integrin receptors, and heparan sulfate receptors to provide references for studies regarding the mechanisms underlying FMDV infection. © 2015 Wang et al. Source


Wang G.,State Key Laboratory of Veterinary Etiological Biology | Wang G.,Chinese Academy of Sciences | Shang Y.,Key Laboratory of Animal Virology of Ministry of Agriculture | Shang Y.,Chinese Academy of Sciences | And 5 more authors.
Virology Journal | Year: 2013

Background: Orf virus (ORFV) causes orf (also known as contagious ecthyma or contagious papular dermatitis), a severe infectious skin disease in goats, sheep and other ruminants. Therefore, a rapid, highly specific and accurate method for the diagnosis of ORFV infections is essential to ensure that the appropriate treatments are administered and to reduce economic losses. Methods. A loop-mediated isothermal amplification (LAMP) assay based on the identification of the F1L gene was developed for the specific detection of ORFV infections. The sensitivity and specificity of the LAMP assay were evaluated, and the effectiveness of this method was compared with that of real-time PCR. Results: The sensitivity of this assay was determined to be 10 copies of a standard plasmid. Furthermore, no cross-reactivity was found with either capripox virus or FMDV. The LAMP and real-time PCR assays were both able to detect intracutaneous- and cohabitation-infection samples, with a concordance of 97.83%. LAMP demonstrated a sensitivity of 89.13%. Conclusion: The LAMP assay is a highly efficient and practical method for detecting ORFV infection. This LAMP method shows great potential for monitoring the prevalence of orf, and it could prove to be a powerful supplemental tool for current diagnostic methods. © 2013 Wang et al.; licensee BioMed Central Ltd. Source


Chen H.-t.,Key Laboratory of Animal Virology of Ministry of Agriculture | Zhang J.,Key Laboratory of Animal Virology of Ministry of Agriculture | Ma Y.-p.,Key Laboratory of Animal Virology of Ministry of Agriculture | Ma L.-n.,Key Laboratory of Animal Virology of Ministry of Agriculture | And 6 more authors.
Molecular and Cellular Probes | Year: 2010

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 101 50% egg infection dose (EID50) per 50 μl of titrated viruses and no cross-reaction of IBV RT-LAMP was found when tested with other viruses including Newcastle disease virus (NDV), avian reovirus (ARV), and infectious laryngotrachietis virus (ILTV) due to their mismatch with IBV RT-LAMP primers. A total of 187 clinical tissues samples (88 blood, 62 kidney and 37 lung) were evaluated and compared to conventional RT-PCR. The sensitivity of RT-LAMP and RT-PCR assays for detecting IBV RNA in clinical specimens was 99.5% and 98.4%, respectively. These findings showed that the RT-LAMP assay has potential usefulness for rapid and sensitive diagnosis in outbreak of IBV. © 2009 Elsevier Ltd. All rights reserved. Source


Nie L.,Zhejiang University | Nie L.,Key Laboratory for Cell and Gene Engineering of Zhejiang Province | Nie L.,Key Laboratory of Animal Virology of Ministry of Agriculture | Xiong R.,Zhejiang University | And 14 more authors.
Developmental and Comparative Immunology | Year: 2014

The suppressor of cytokine signaling 1 (SOCS-1) protein is a critical regulator in the immune systems of humans and mammals, which functions classically as an inhibitor of the IFN signaling pathways. However, data on functional characterisation of SOCS-1 in ancient vertebrates are limited. In this study, we report the function of teleost SOCS-1s in IFN signaling in fish models (zebrafish and Tetraodon) and human cells. Structurally, teleost SOCS-1s share conserved functional domains with their mammalian counterparts. Functionally, teleost SOCS-1s could be significantly induced upon stimulation with IFN stimulants and zebrafish IFNφ1. Overexpression of teleost SOCS-1s could dramatically suppress IFNφ1-induced Mx, Viperin and PKZ activation in zebrafish, and IFN-induced ISG15 activation in HeLa cells. Furthermore, a SOCS-1 variant that lacks the KIR domain was also characterised. This study demonstrates the conserved negative regulatory role of teleost SOCS-1s in IFN signaling pathways, providing perspective into the functional conservation of SOCS-1 proteins during evolution. © 2013 Elsevier Ltd. Source


Li D.,Key Laboratory of Animal Virology of Ministry of Agriculture | Li D.,Chinese Academy of Agricultural Sciences | Bai X.-W.,Key Laboratory of Animal Virology of Ministry of Agriculture | Bai X.-W.,Chinese Academy of Agricultural Sciences | And 14 more authors.
Acta Virologica | Year: 2010

Tree different routes of Foot-and-mouth disease virus (FMDV) infection of piglets, namely intranasal (i.n.) through drops, intradermal (i.d.) into the foot, and intramuscular (i.m.) were compared regarding the onset and severity of the disease. Te results showed that the i.d. injection of the virus resulted in the fastest onset of the disease. Te i.m. injection led to a delayed onset, but the final effect was identical with i.d. injection. Moreover, the i.m. injection was simpler to perform and easier to evaluate. Therefore, the i.m. injection of piglets is recommended as the optimal infection route for evaluation of the FMDV vaccine potency. Source

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