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Qi X.,Northwest University, China | Qi X.,Key Laboratory of Animal Reproductive Endocrinology and Engineering | Jin Y.,Northwest University, China | Jin Y.,Key Laboratory of Animal Reproductive Endocrinology and Engineering | And 3 more authors.
Journal of Animal and Veterinary Advances | Year: 2011

Microcapsules of Lactobacillus brevis were prepared using spray drying technique and the resistance of the microencapsulated form of this microorganism to drying at high temperature and simulated gastrointestinal conditions were evaluated. The therapeutic effect of these microcapsules on the diarrhea of neonatal calf was also evaluated using ERIC-PCR. The results showed that the completely release of the encapsulated bacteria took place after 60 nun in contact with simulated intestinal conditions. Microencapsulated bacteria were resistant at least for 90 min to simulated gastric juice (pH 1.2) or simulated intestinal juice (pH 7.2) and microencapsulated form of the microorganisms was more resistant to drying at 85°C than free form. ERIC-PCR profiles of diarrhea calves treated with probiotic capsules were similar to that of control health calves at 3 days post administration suggesting that administration of these probiotic capsules may have strong positive effect on the treatment of neonatal calf diarrhea. © Medwell Journals, 2011. Source

Qi X.,Key Laboratory of Animal Reproductive Endocrinology and Engineering | Qi X.,Northwest University, China | Bai D.,Northwest University, China | Zhao X.,Northwest University, China | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2011

The ability of three Lactic Acid Bacteria (LAB), i.e., Lactobacillus acidophilus, Enterococcus faecalis and Enterococcus cecorum that isolated from cervical mucus in health cattle to adhere to immortalized Endometrial Epithelial Cells (EEC) and to affect the adhesion of two main endometritis-associated pathogens, Escherichia coli and Staphylococcus aureus were investigated. All probiotic strains exhibited strong adhesion levels to the cell lines and the adhesion levels of L. acidophilus was significantly higher as compared to that of E. faecalis or E. cecorum (p<0.05). In coculture assays, a significant decrease in the number of adhering pathogens was observed, using either preincubation, postincubation or coincubation of the pathogens with these LAB. L.acidophilus exhibited strong antagonistic activity against E. coli (p<0.01), nevertheless, E. Faecalis and ii. cecorum showed strong inhibitory effect on S. aureus (p<0.01). In conclusion, the ability of LAB tested to inhibit the adhesion of endometritis-associated pathogens is highly specific and depends on both the probiotic and pathogen strains. © Medwell Journals, 2011. Source

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