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Guo X.-Q.,Key Laboratory of Animal Physiology | Guo X.-Q.,Peking University | Guo X.-Q.,Bethune Military Medical College | Lu J.-X.,Peking University | And 5 more authors.
Acta Anatomica Sinica | Year: 2011

Objective: To investigate the expressions of H3K9 methyltransferases (KMTs) and demethylases (KDMs) in the liver of different aged mice. Methods: Two-week, four-week and six-week old mice were killed and their liver tissues collected. Both mRNA and protein from the mice liver were extracted using Trizol kit and sonicated method respectively. Reverse transcription-quantitative real time polymerase chain reaction (RT-qPCR) was performed for mRNA expressions of several kinds of H3K9 methyltransferases and demethylases. The levels of H3K9 demethylases KDM3A and KDM4B proteins were determined using Western blotting. Results: The mRNA expressions of several KMTs (including KMT1C, KMT1E and PRDM2) and KDMs (including KDM3A, KDM3B, KDM4A and KDM4B) were obviously different in the different developmental stages of a mouse's liver (P < 0.05, n = 5). The levels of KDM3A and KDM4B proteins in the mice liver were also distinct, and both proteins in six-week old mice were lower than those in two-week and four-week old mice (P < 0.05, n = 3). Conclusion: The H3K9 methylation status of specific gene loci may be closely associated with liver development and biological function. Source

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