Jiang Y.,Key Laboratory of Animal Origin Food Production and Safety Guarantee |
Jiang Y.,Nanjing Agricultural University |
Jiang Y.,Nanjing Normal University |
Zhang W.H.,Key Laboratory of Animal Origin Food Production and Safety Guarantee |
And 5 more authors.
Animal Production Science | Year: 2015
The aim of the present study was to investigate the effects of micro-encapsulated sodium butyrate (MSB) on oxidative stress and apoptosis induced by dietary corticosterone (CORT) in the intestinal mucosa of broiler chickens. In total, 120 1-day-old male broilers (Arbor Acres) were randomly allocated to two treatment groups and were fed on a control diet (without MSB) or 0.4 g MSB/kg diet. Each treatment had six replicates with five chickens each. From 7 days of age onward, 50% of the chickens in each dietary treatment were subjected to CORT treatment (30 mg/kg of diet). The experimental period was 21 days. The results showed that CORT administration decreased (P < 0.001) feed intake and bodyweight gain and increased (P < 0.001) feed to gain ratio (F:G) of broiler chickens. The dietary MSB supplementation decreased (P < 0.01) F:G and there was an interaction between MSB and CORT on F:G (P < 0.05). Moreover, the activities of superoxide dismutase, glutathione peroxidase and catalase in intestinal mucosa were decreased (P < 0.01 or P < 0.001), and the concentrations of malondialdehyde in the intestinal mucosa were elevated (P < 0.01) by CORT administration. In contrast, treatment of MSB increased (P < 0.01) the catalase activities in duodenal and jejunal mucosa and decreased (P < 0.01) the malondialdehyde concentrations in duodenal mucosa. Higher apoptosis index and lower mRNA expressions of bcl-2 in intestinal epithelial cells were induced (P < 0.05) by CORT treatment. However, MSB decreased (P < 0.05) the apoptosis index and increased the bcl-2 expression. These results suggest that dietary MSB can partially attenuate oxidative stress induced by CORT treatment and inhibit apoptosis of intestinal epithelial cells in broiler chickens. © CSIRO 2015.