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Sun Y.,Gansu Agricultural University | Xing G.,Key Laboratory of Animal Immunology of the Ministry of Agriculture Henan Academy of Agricultural science Zhengzhou 450002 China | Yang J.,Key Laboratory of Animal Immunology of the Ministry of Agriculture Henan Academy of Agricultural science Zhengzhou 450002 China | Wang F.,Key Laboratory of Animal Immunology of the Ministry of Agriculture Henan Academy of Agricultural science Zhengzhou 450002 China | And 4 more authors.
Journal of the Science of Food and Agriculture | Year: 2015

BACKGROUND: Ochratoxin A (OTA) and zearalenone (ZEN) are natural products of filamentous fungi that are harmful to humans and animals exposed to them even in extremely low concentration. The immunochromatographic test strip has become a popular diagnostic tool for detecting analytes. Its major advantages are that results can be obtained within 5-10 min, all needed reagents are included in the strip and it can be used to detect OTA and ZEN contamination in spots. In this study a colloidal gold-based immunochromatographic test strip of competitive format was developed for the rapid simultaneous qualitative and quantitative detection of OTA and ZEN in corn and other cereals. RESULTS: The test strip results with the naked eye showed that the sensitivities were 6 μg kg-1 OTA and 20 μg kg-1 ZEN in cereal, while those with a TSR3000 membrane strip reader showed that the IC50 values of OTA and ZEN were 1.7905 and 4.3514 ng mL-1 and the lower detection limit (LDL) values were 0.7697 and 1.2000 μg kg-1 respectively. These results were confirmed by high-performance liquid chromatography. CONCLUSION: The immunochromatographic test strip developed in this study could be used for the rapid simultaneous, qualitative and quantitative screening of OTA and ZEN in corn samples. © 2015 Society of Chemical Industry.

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