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Chen H.,Northwest University, China | Chen H.,Key Laboratory of Animal Biotechnology of the Ministry of Agriculture | Zhang L.,Northwest University, China | Zhang L.,Key Laboratory of Animal Biotechnology of the Ministry of Agriculture | And 12 more authors.
Molecular Reproduction and Development | Year: 2015

Vitamin C (Vc) has been widely studied in cell and embryo culture, and has recently been demonstrated to promote cellular reprogramming. The objective of this study was to identify a suitable Vc concentration that, when used to treat adult bovine fibroblasts serving as donor cells for nuclear transfer, improved donor-cell physiology and the developmental potential of the cloned embryos that the donor nuclei were used to create. A Vc concentration of 0.15mM promoted cell proliferation and increased donor-cell 5-hydroxy methyl cytosine levels 2.73-fold (P<0.05). The blastocyst rate was also significantly improved after nuclear transfer (39.6% treated vs. 26.0% control, P<0.05); the average number of apoptotic cells in cloned blastocysts was significantly reduced (2.2 vs. 4.4, P<0.05); and the inner cell mass-to-trophectoderm ratio (38.25% vs. 30.75%, P<0.05) and expression of SOX2 (3.71-fold, P<0.05) and POU5F1 (3.15-fold, P<0.05) were significantly increased. These results suggested that Vc promotes cell proliferation, decreases DNA methylation levels in donor cells, and improves the developmental competence of bovine somatic-cell nuclear transfer embryos. © 2015 Wiley Periodicals, Inc.

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