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Li J.-T.,Key Laboratory of Analytical Science and Technology | Sun S.-F.,Key Laboratory of Analytical Science and Technology
E-Journal of Chemistry | Year: 2010

The synthesis of diindolylmethanes (DIMs) via the condensation of indole and aromatic aldehydes catalyzed by silicotungstic acid was carried out in 68%~98% yields at room temperature within 1~5 min by grinding. This method provides several advantages such as operational simplicity, higher yield and environment friendly.

Zhao Y.,Hebei University | Zhao Y.,Key Laboratory of Analytical Science and Technology | Zhao Y.,Key Laboratory of Chemical Biology | Liu L.,Hebei University | And 8 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2013

An analytical method for the simultaneous determination of 18α-glycyrrhizic acid, 18β-glycyrrhizinic acid, related substances A and B and drug quality standard by reversed-phase high performance liquid chromatography (RP-HPLC) was established. The assay was carried out on a Durashell-Cl8 column (250 mm ×4. 6 mm, 5 μm) with 10 mmol/L ammonium perchlo-rate (the pH value was adjusted to 8.20 with ammonia)-methanol (48: 52, v/v) as mobile phase at a flow rate of 0. 80 mL/min, and the detection wavelength was set at 254 nm. The column temperature was 50 °C and the injection volume was 10 (xL. Under the separation conditions, the calibration curves of the analytes showed good linearities within the mass concentrations of 0. 50 - 100 mg/L (r >0. 999 9). The detection limits for 18α-glycyrrhizic acid, 18β-gly-cyrrhizinic acid, related substances A and B were 0. 15, 0. 10, 0. 10, 0. 15 mg/L, respectively. The average recoveries were between 97. 32% and 99. 33% (n = 3) with the relative standard deviations (RSDs) between 0. 05% and 1. 06%. The method is sensitive, reproducible, and the results are accurate and reliable. The method can be used for the determination of principal components and related substances of ammonium glycyrrhizinate for the quality control of raw material drug of ammonium glycyrrhizinate.

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