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Liu M.,Shaanxi Normal University | Li B.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province
Microchimica Acta | Year: 2016

The water-soluble conjugated polymer poly[(9,9-di(3,3-N,N-trimethylammonium)propylfluorenyl-2,7-diyl)-alt-co-(1,4-phenylene)] dibromide (referred to as PFP+) is shown to represent a useful reagent for the detection of DNA hybridization. The intensity of the chemiluminescence (CL) of the luminol-H2O2 system is strongly enhanced in the presence of PFP+, but this enhancement is inhibited after electrostatic interaction between PFP+ and DNA as shown for oligomers of lengths between 12 and 35 bases. CL decreases more strongly in the presence of ds-DNA than in the presence of ss-DNA. Hybridization between probe DNA and target DNA results in a weaker effect of PFP+ and in reduced CL emission. These finding are exploited in a homogeneous CL based assay for medium-length DNA (12 to 35 bp). The detection limit (at a signal-to-noise ratio of 3σ) is 3.0 pM. © 2015, Springer-Verlag Wien.


Zhang L.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province | Guo Z.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province | Zheng X.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province | Wang R.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province | And 4 more authors.
Acta Chimica Sinica | Year: 2011

A novel mesoporous chitosan-silica nanoparticles was synthesized with the water-in-oil (W/O) reverse microemulsion method. By characterizing this nanoparticles with transmission electron microscopy, IR spectra and the zeta potential experiments, it was found that those nanoparticles were spherical and uniform with 50 nm size and presented the mesoporous structure. Based on these findings, the adsorption ability of this nanoparticles for calf thymus DNA was studied. The relating results showed that this nanoparticles had a stronger adsorption ability to DNA in PBS buffer (pH=6), they also presented the better biocompatibility for cell and could be used for protect DNA from Dnase I cleavage.


Li Y.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province | Li B.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province | Zhang J.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province
Luminescence | Year: 2013

A novel fluorescence assay system for glucose was developed with thioglycollic acid (TGA)-capped CdTe quantum dots (QDs) as probes. The luminescence quantum yield of the TGA-capped CdTe QDs was highly sensitive to H2O2 and pH. In the presence of glucose oxidase, glucose is oxidized to yield, gluconic acid and H2O2. H 2O2 and H+ (dissociated from gluconic acid) intensively quenched the fluorescence of QDs. The experimental results showed that the quenched fluorescence was proportional to the glucose concentration within the range of 0.01-5.0 mm under optimized experimental conditions. Compared with most of the existing methods, this newly developed system possesses many advantages, including simplicity, low cost, high flexibility, and good sensitivity. Furthermore, no complicated chemical modification of QDs and enzyme immobilization was needed in this system. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.


Qi L.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province | Qi L.,Shaanxi Normal University | Huo Y.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province | Wang H.,Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province | And 4 more authors.
Analytical Chemistry | Year: 2015

Studying ligand-biomacromolecule interactions provides opportunities for creating new compounds that can efficiently regulate specific biological processes. Ribonucleic acid (RNA) molecules have become attractive drug targets since the discovery of their roles in modulating gene expression, while only a limited number of studies have investigated interactions between ligands and functional RNA molecules, especially those based on nanotechnology. DNA-protected silver nanoclusters (AgNCs) were used to investigate ligand-RNA interactions for the first time in this study. The anthracycline anticancer drug mitoxantrone (MTX) was found to quench the fluorescence of AgNCs. After adding human immunodeficiency virus trans-activation responsive region (TAR) RNA or Rev-response element (RRE) RNA to AgNCs-MTX mixtures, the fluorescence of the AgNCs recovered due to interactions between MTX with RNAs. The binding constants and number of binding sites of MTX to TAR and RRE RNA were determined through theoretical calculations. MTX-RNA interactions were further confirmed in fluorescence polarization and mass spectrometry experiments. The mechanism of MTX-based fluorescence quenching of the AgNCs was also explored. This study provides a new strategy for ligand-RNA binding interaction assay. © 2015 American Chemical Society.

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