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Fang H.,State Key Laboratory of Analytical Chemistry for Life Science
Lecture Notes in Engineering and Computer Science | Year: 2013

In order to fabricate high speed and low cost ADC, a new type Analog-to-Digital converter (ADC) was presented on the basis of the periodicity of trigonometric function. This kind of ADC is defined as T-ADC. Each bit conversion of T-ADC is performed nearly in parallel. An N-bit T-ADC consists of a trigonometric function circuit, N 1 groups of analog multipliers and operational amplifiers, N comparators, and a decoder. The input voltage is mapped to cosine of the corresponding angle first. Then the angle in cosine function is doubled N -1 times. All the cosine function voltages of the doubled angles are used to generate the result of A/D conversion. Theoretically, T-ADC can achieve the conversion speed of flash ADC. In this paper, the sources of error in T-ADC and their influences on T-ADC are analyzed. Based on it, an error reduction approach in T-ADC is presented.

Lin Y.,Anhui Polytechnic University | Lin Y.,Nanjing University | Ding Y.,Nanjing University | Ding Y.,State Key Laboratory of Analytical Chemistry for Life Science | Jiang X.,Nanjing University
Progress in Chemistry | Year: 2014

Polymer-inorganic hybrid nanospheres have attracted increasing attention in recent years because of the synergic properties arising from both the polymeric nanospheres and inorganic nanomaterials. Especially, the nanospheres composed by the polymers that have desirable plasticity and biocompatibility, and the inorganic materials with unique optical, magnetic and electric properties are greatly useful in diagnosis and therapy disease. The combinations of functional polymers, inorganic nanomaterials and bioactive molecules can offer synergetic multifunctional nanomedical platforms, which make it possible to accomplish multimodal imaging and monitoring therapy. This article provides a review on the synthetic methodologies for building hybrid nanospheres, and their applications in targeted drug delivery, bio-imaging, cell separation, biosensing and hyperthermia. Perspective and challenges in nanomedical fields are discussed to provide the reference information for development of novel theranostic hybrid nanospheres.

Xu S.,Nanjing University | Zou B.,Nanjing University | Xiang Z.,China Pharmaceutical University | Miao M.,Nanjing Medical University | And 5 more authors.
Analytical Methods | Year: 2016

Non-invasive detection of trisomy 21 is a safe and effective way for prenatal diagnosis. Although using next generation sequencing technology can achieve non-invasive detection of chromosomal abnormalities, a more convenient and cost-effective method is preferable for routine clinical applications. Here, we proposed a novel method for the detection of trisomy 21 by accurately quantifying the slightly increased amount of chromosome 21 in cell-free DNA from maternal plasma using digital PCR. The segmental duplication fragments on chromosome 21 and chromosome 1 were employed as the detection target of digital PCR. As low as 10% cell-free fetal DNA of trisomy 21 fetus in maternal cell-free DNA was successfully detected. Three trisomy 21 samples were unambiguously picked up from 15 clinical samples, indicating that our method has the potential for non-invasive diagnosis of trisomy 21. © The Royal Society of Chemistry 2016.

Ding Y.,State Key Laboratory of Analytical Chemistry for Life Science | Li Y.,Nanjing University | Li Y.,Nanjing University of Information Science and Technology | Qin M.,Nanjing University | And 2 more authors.
Langmuir | Year: 2013

Peptide-based supramolecular hydrogels have been extensively explored in biomaterials owing to their unique bioactive, stimulus-responsive, and biocompatible features. However, peptide-based hydrogels often have low mechanical stability with storage moduli of 10-1000 Pa. They are susceptible to mechanical destruction and solvent erosion, greatly hindering their practical application. Here, we present a photo-cross-linking strategy to enhance the mechanical stability of a peptide-based hydrogel by 104-fold with a storage modulus of ∼100 kPa, which is one of the highest reported so far for hydrogels made of small peptide molecules. This method is based on the ruthenium-complex-catalyzed conversion of tyrosine to dityrosine upon light irradiation. The reinforcement of the hydrogel through photo-cross-linking can be achieved within 2 min thanks to the fast reaction kinetics. The enhancement of the mechanical stability was due to the formation of a densely entangled fibrous network of peptide dimers through a dityrosine linkage. We showed that in order to implement this method successfully, the peptide sequence should be rationally designed to avoid the cross talk between self-assembly and cross-linking. This method is convenient and versatile for the enhancement of the mechanical stability of tyrosine-containing peptide-based hydrogels. We anticipate that the photo-cross-linked supramolecular hydrogels with much improved mechanical stability will find broad applications in tissue engineering and drug controlled release. © 2013 American Chemical Society.

Huang Z.,State Key Laboratory of Pharmaceutical Biotechnology | Wang Z.,State Key Laboratory of Pharmaceutical Biotechnology | Long S.,State Key Laboratory of Pharmaceutical Biotechnology | Jiang H.,State Key Laboratory of Pharmaceutical Biotechnology | And 5 more authors.
Molecular Pharmaceutics | Year: 2014

Functional engineered nanoparticles are promising drug delivery carriers. As the construction of a functional nanocarrier always needs the optimization of multiple technical variables, efficient in vitro high-throughput evaluation methods would help to shorten the development cycle. In the present study, we generated a tissue mimic of the colon of inflammatory bowel disease (IBD) patients. Generally, Caco-2 cells and THP-1 cells were grown in a 3-D matrix with different number, spatial distribution and specific extracellular cell matrix (ECM) composition according to real healthy and inflamed animal colon tissues. After interlerukin-1β/lipopolysaccharide (LPS) stimulation, the artificial model closely resembled the pathological features of IBD patients colon, including massive cytokines and mucus production, epithelium defect and leukocytic infiltration. The tissue and cellular uptake of three different nanoparticles in the artificial model was similar to that in 2,4,6-trinitrobenzenesulfonic acid (TNBS) colitic mice. Most importantly, our artificial tissue can be placed into 96-well plates for high-throughput screening of drug delivery carriers for the treatment of IBD. Our study suggested a readily achievable way to improve current methodologies for the development of colon targeted drug delivery systems. © 2014 American Chemical Society.

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