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Qiu J.,Key Laboratory of Food Quality and Safety of Jiangsu Province | Qiu J.,Key Laboratory of Control Technology and Standard for Agro product Safety and Quality Nanjing | Qiu J.,Collaborative Innovation Center for Modern Grain Circulation and Safety | Qiu J.,Key Laboratory of Agro product Safety Risk Evaluation Nanjing | And 17 more authors.
European Journal of Plant Pathology | Year: 2015

In 2012, we isolated mycotoxigenic fungi from maize kernels sourced in Jiangsu and Anhui provinces, China. Fusarium spp. accounted for most isolates detected, followed by Aspergillus and Penicillium spp. Of the Fusarium species, Fusarium verticillioides was the most prevalent, as revealed by direct PCR of DNA from kernel samples, followed by F. proliferatum and F. graminearum. Most F. verticillioides strains contained the FUM1 gene, the product of which plays a key role in fumonisin biosynthesis. In terms of mating type idiomorphs, about 80 % of all strains carried the MAT-1 allele, and 20 % carried MAT-2. The genetic structures of 192 representative F. verticillioides isolates were determined via an analysis of eight simple sequence repeat markers. This analysis revealed high-level gene diversity but low linkage disequilibrium in three populations grouped by the geographical areas in which they were collected. The three populations exhibited low-level genetic differentiation and high gene flow, indicating that geographical differences minimally influenced population differentiation; all three populations were potentially components of a larger, randomly mating population. © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging.


Wei R.,Nanjing Agricultural University | Wei R.,Key Laboratory of Agro product Safety Risk Evaluation Nanjing | Ge F.,Nanjing Institute of Environmental Sciences | Zhang L.,Key Laboratory of Agro product Safety Risk Evaluation Nanjing | And 6 more authors.
Chemosphere | Year: 2016

The occurrence of 13 veterinary drugs were studied in soil fertilized with animal manures in Eastern China. The 69 soil samples were obtained from twenty-three vegetable fields in 2009 and analysed for selected veterinary drugs by HPLC-MS/MS at soil depths of 0-20, 20-40 and 40-60 cm, and two additional samples were re-analysed from an earlier study from November 2011. Results showed that animal wastes, especially those from poultry farms, were one of pollution sources of veterinary drugs in soil. The detection frequency of veterinary drugs in soil was 83%, 91% and 87% in the three soil depths, respectively. The detection rates for the five classes of drugs in soils followed the rank order cyromazine > tetracyclines > sulfonamides > fluoroquinolones > florfenicol. Veterinary drugs were detected in soil layers at 20-40 and 40-60 cm depth to a greater extent than at 0-20 cm depth. The results of the same point in years 2009 and 2011 indicated that veterinary drugs accumulate easily and persist in the deeper soil. In addition, residue levels of veterinary drugs in soil were related to the animal species the manure was derived from. Overall, the predominance of tetracyclines in sampled soils underscored the need to regulate their veterinary use in order to improve the management and treatment of associated releases. © 2015 Elsevier Ltd.


PubMed | Nanjing Institute of Environmental Sciences, Key Laboratory of Agro product Safety Risk Evaluation Nanjing and Nanjing Agricultural University
Type: | Journal: Chemosphere | Year: 2015

The occurrence of 13 veterinary drugs were studied in soil fertilized with animal manures in Eastern China. The 69 soil samples were obtained from twenty-three vegetable fields in 2009 and analysed for selected veterinary drugs by HPLC-MS/MS at soil depths of 0-20, 20-40 and 40-60 cm, and two additional samples were re-analysed from an earlier study from November 2011. Results showed that animal wastes, especially those from poultry farms, were one of pollution sources of veterinary drugs in soil. The detection frequency of veterinary drugs in soil was 83%, 91% and 87% in the three soil depths, respectively. The detection rates for the five classes of drugs in soils followed the rank order cyromazine > tetracyclines > sulfonamides > fluoroquinolones > florfenicol. Veterinary drugs were detected in soil layers at 20-40 and 40-60 cm depth to a greater extent than at 0-20 cm depth. The results of the same point in years 2009 and 2011 indicated that veterinary drugs accumulate easily and persist in the deeper soil. In addition, residue levels of veterinary drugs in soil were related to the animal species the manure was derived from. Overall, the predominance of tetracyclines in sampled soils underscored the need to regulate their veterinary use in order to improve the management and treatment of associated releases.


Lu H.-Y.,Jiangsu Academy of Agricultural Sciences | Lu H.-Y.,Key Laboratory of Control Technology and Standard for Agro product Safety and Quality | Lu H.-Y.,Key Laboratory of Agro product Safety Risk Evaluation Nanjing | Shen Y.,Jiangsu Academy of Agricultural Sciences | And 11 more authors.
Journal of the Science of Food and Agriculture | Year: 2013

Background: The presence of pesticide residues in food has caused much concern. The low health risks and environmental impacts of limonene make it a very interesting solvent for use in green chemistry. Washing effects of limonene on pesticide residues of methyl chlorpyrifos, chlorothalonil, chlorpyrifos, fenpropathrin and deltamethrin were investigated in green pepper. Results: Results showed that washing with a low concentration of limonene for 5 min (where LOQ is limit of quantitation) caused 53.67%,


Wang T.-T.,Key Laboratory of Agro product Safety Risk Evaluation Nanjing | Wang T.-T.,Guangxi University | Cheng J.,Key Laboratory of Agro product Safety Risk Evaluation Nanjing | Liu X.-J.,Key Laboratory of Agro product Safety Risk Evaluation Nanjing | And 3 more authors.
Ecotoxicology and Environmental Safety | Year: 2012

To evaluate the effect of biochar amendment on the bioavailability of chlorantraniliprole (CAP) in soils with different physico-chemical properties, the uptake of CAP from various soils by earthworms was studied. It was observed that the biochar amendment of the soils affected the sorption of CAP, but the magnitude of the sorption enhancement by biochar amendment among the soils was varied, presumably due to the attenuation of the sorptivity of the biochar when amended in the soil. The amendment with biochars leads to a decrease in the bioavailability of CAP in the soils to earthworms, and more prominent for biochar BC850 amendment. In the soil with a CAP concentration of 10mgkg-1, the residue of CAP in the earthworm tissues was found to be 9.65mgkg-1, in comparison with that the CAP residue was 4.05mgkg-1 in BC450 amended soil and 0.59mgkg-1 in BC850, respectively. The degree of bioavailability reduction by same level of biochar amendment was different among soils with different properties. The results demonstrate that the properties of soils are important to performance of biochar in soil. © 2012 Elsevier Inc.


Ji F.,State Key Laboratory Breeding Base of Food Quality and Safety in Jiangsu Province | Ji F.,Key Laboratory of Control Technology and Standardfor Agro product Safety and Quality | Ji F.,Key Laboratory of Agro product Safety Risk Evaluation Nanjing | Ji F.,Jiangsu Academy of Agricultural Sciences | And 16 more authors.
Toxins | Year: 2015

A total of 122 wheat varieties obtained from the Nordic Genetic Resource Center were infected artificially with an aggressive Fusariumasiaticum strain in a field experiment. We calculated the severity of Fusarium head blight (FHB) and determined the deoxynivalenol (DON) content of wheat grain, straw and glumes. We found DON contamination levels to be highest in the glumes, intermediate in the straw, and lowest in the grain in most samples. The DON contamination levels did not increase consistently with increased FHB incidence. The DON levels in the wheat varieties with high FHB resistance were not necessarily low, and those in the wheat varieties with high FHB sensitivity were not necessarily high. We selected 50 wheat genotypes with reduced DON content for future research. This study will be helpful in breeding new wheat varieties with low levels of DON accumulation. © 2015 by the authors; licensee MDPI, Basel, Switzerland.

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