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Fan X.,Huazhong Agricultural University | Fan X.,Key Laboratory of Agro Microbial Resource and Development | Zhou Y.,Huazhong Agricultural University | Zhou Y.,Key Laboratory of Agro Microbial Resource and Development | And 4 more authors.
World Journal of Microbiology and Biotechnology | Year: 2014

Two allelic variants of the gpd gene, Gpd a and Gpd b, were isolated based on a putative glyceraldehyde-3-phosphate dehydrogenase encoding sequence from the transcriptome of Auricularia auricula-judae strain Au916. The two alleles were found to have a 73 bp length discrepancy and 39 SNP variations. Both of the genomic DNA sequences of two alleles were interrupted by five introns, and encoded a same 340 aa protein. Intron positions analysis showed that the first intron was absent, but the last unique intron was gained in A. auricula-judae. Allele-specific expression analysis showed that the Gpd a and Gpd b were expressed with no significant difference in dikaryotic mycelia of A. auricula-judae. To the best of our knowledge, this is the first report about the detection of two allelic gpd genes in A. auricula-judae, as well as the application of allele-specific primers in gene expression analysis for this edible fungus. © 2013 Springer Science+Business Media Dordrecht.


Fan X.,Huazhong Agricultural University | Fan X.,Key Laboratory of Agro Microbial Resource and Development | Zhou Y.,Huazhong Agricultural University | Zhou Y.,Key Laboratory of Agro Microbial Resource and Development | And 6 more authors.
Microbiological Research | Year: 2014

Laccases (p-diphenol: oxygen oxidoreductase; EC 1.10.3.2) are multi-copper oxidases encoded by gene family in white rot fungi. Auricularia auricula-judae is one kind of white rot fungi with a soft, jelly-like texture and an ear-like shape. In the present study, seven laccase genes containing the signature sequences L1-L4 were isolated from A. auricula-judae strain Au916 on the basis of the mycelium-derived transcriptome. In the basidiomycetes, the predicted substrate binding loops of the A. auricula-judae laccases were found to be uncommon. Phylogenetic analysis showed that the laccases of the Auricularia were nested into the ascomycete laccases, indicating that the laccase genes from Auricularia are distinctly different in function from other basidiomycetes. Among the seven laccases, the intron positions and cluster distributions in the NJ tree varied from each other and the expression patterns of seven genes estimated by qRT-PCR were also discrepant. The lcc3 gene was highly expressed not only in the free-living mycelium but also in substrate mycelium, furthermore, the lcc5 gene was mostly expressed during the fruiting body formation and maturation indicating that lcc5 might play a major role during the sexual reproduction stage. © 2013 Elsevier GmbH.

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