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He H.,Northeast Agricultural University | Liu C.,Northeast Agricultural University | Zhao J.,Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee | Li W.,The Key Laboratory for Microbial Resources of the Ministry of Education | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A novel endophytic actinomycete, designated strain NEAU-LZS-5T, was isolated from the leaf of candelabra aloe (Aloe arborescens Mill) and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain NEAU-LZS-5T belongs to the genus Streptomyces and exhibited 99.51 and 97.37% similarity to Streptomyces sedi YIM 65188T and Streptomyces specialis GW41-1564T, respectively, whereas low similarity values (<97 %) distinguished strain NEAU-LZS-5T from all other species of the genus Streptomyces with validly published names. Two tree-making algorithms also supported the position that strain NEAU-LZS- 5T formed a distinct clade with Streptomyces sedi YIM 65188T and Streptomyces specialis GW41-1564T. However, levels of DNA-DNA relatedness between strain NEAU-LZS-5T and Streptomyces sedi YIM 65188T and Streptomyces specialis GW41-1564T were 45.59 and 31.90 %, respectively. A comparative study between strain NEAU-LZS-5T and the type strains of closest related species of the genus Streptomyces revealed that it differed from them in morphological, physiological and biochemical characteristics. Therefore, strain NEAU-LZS-5T represents a novel species of the genus Streptomyces, for which the name Streptomyces zhaozhouensis sp. nov. is proposed. The type strain is NEAU-LZS-5T (=CGMCC 4.7095T=DSM 42101T). © 2014 IUMS.


Zhang X.,Northeast Agricultural University | Zhang Y.,Northeast Agricultural University | Zhao J.,Northeast Agricultural University | Liu C.,Northeast Agricultural University | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A novel actinomycete, designated strain NEAU-dht8T, was isolated from the rhizosphere soil of rehmannia (Rehmannia glutinosa Libosch) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Nonomuraea. The G+C content of the DNA was 68.47 mol%. On the basis of 16S rRNA gene sequence similarity studies, strain NEAU-dht8T was most closely related to Nonomuraea maheshkhaliensis 16-5-14T (99.31 %), Nonomuraea kuesteri GW 14-1925T (98.77 %), Nonomuraea coxensis JCM 13931T (98.71 %), Nonomuraea wenchangensis 210417T (98.44 %), Nonomuraea bangladeshensis 5-10-10T (98.36 %) and Nonomuraea salmonea DSM 43678T (98.0 %); similarities to other species of the genus Nonomuraea were lower than 98 %. Two tree-making algorithms based on 16S rRNA gene sequences showed that the isolate formed a phyletic line with its closest neighbour N. maheshkhaliensis 16-5-14T. However, the low level of DNA-DNA relatedness allowed the novel isolate to be differentiated from N. maheshkhaliensis 16-5-14T. Strain NEAU-dht8T could also be differentiated from other species of the genus Nonomuraea showing high 16S rRNA gene sequence similarity (98-98.77 %) by morphological and physiological characteristics. Thus, strain NEAU-dht8T is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea fuscirosea sp. nov. is proposed. The type strain is NEAU-dht8T (=CGMCC 4.7104T=DSM 45880T). © 2014 IUMS.


Zhou Y.,Northeast Agricultural University | Liu C.,Northeast Agricultural University | Zhang Y.,Northeast Agricultural University | Li C.,Northeast Agricultural University | And 6 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2014

A Gram-stain positive, filamentous bacterial strain, designated strain NEAU-TWSJ13T, was isolated from the rhizosphere of a marigold (Tagetes erecta L.) plant collected in Heilongjiang Province, northeast China, and characterized using a polyphasic approach. The strain was observed to form abundant aerial hyphae differentiated into spherical sporangia. 16S rRNA gene sequence similarity studies showed that strain NEAU-TWSJ13T belongs to the genus Streptosporangium, being most closely related to Streptosporangium fragile DSM 43847T (98.6 %). Phylogenetic analysis of the 16S rRNA gene sequence indicated that it formed a phyletic line with S. fragile DSM 43847T, Streptosporangium jomthongense NBRC 110047T (98.4 % 16S rRNA gene similarity) and Streptosporangium violaceochromogenes DSM 43849T (97.6 % 16S rRNA gene similarity). A combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-TWSJ13T can be distinguished from S. fragile DSM 43847T and S. jomthongense NBRC 110047T. Moreover, strain NEAU-TWSJ13T can also be differentiated from S. violaceochromogenes DSM 43849T and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. Therefore, it is proposed that strain NEAU-TWSJ13T represents a novel species of the genus Streptosporangium, for which the name Streptosporangium subfuscum sp. nov. is proposed. The type strain is NEAU-TWSJ13T ( = CGMCC 4.7146T = DSM = 46724T). © 2014, Springer International Publishing Switzerland.


PubMed | Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee
Type: Journal Article | Journal: International journal of systematic and evolutionary microbiology | Year: 2014

A novel actinomycete, designated strain NEAU-GRX6T, was isolated from mucky soil collected from a stream of Jinlong Mountain in Harbin, Heilongjiang Province, north China, and characterized using a polyphasic approach. The isolate formed irregular sporangia containing motile sporangiospores on the substrate mycelium. The whole-cell sugars were xylose, glucose and galactose. The predominant menaquinones were MK-9(H6), MK-10(H4) and MK-9(H4). The major fatty acids were C16:0, C15:0, C18:19c, C17:17c and C18:0. The phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The DNA G+C content was 67 mol%. 16S rRNA gene sequence similarity studies showed that strain NEAU-GRX6T belonged to the genus Actinoplanes, being most closely related to Actinoplanes palleronii IFO 14916T (97.80% similarity) and Actinoplanes missouriensis NBRC 102363T (97.76%). However, the low observed levels of DNA-DNA relatedness allowed the isolate to be differentiated from the above-mentioned species of the genus Actinoplanes. Moreover, strain NEAU-GRX6T could also be distinguished from A. palleronii IFO 14916T and A. missouriensis NBRC 102363T by phenotypic characteristics. Therefore, it is proposed that strain NEAU-GRX6T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes lutulentus sp. nov. is proposed. The type strain is strain NEAU-GRX6T (=CGMCC 4.7090T=DSM 45883T).

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