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Li C.,Northeast Agricultural University | Liu C.,Northeast Agricultural University | Zhao J.,Northeast Agricultural University | Zhang Y.,Northeast Agricultural University | And 5 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2014

A novel actinomycete, designated strain NEAU-MES19T, was isolated from pine forest soil in Heilongjiang province, China. A polyphasic study was carried out to establish the taxonomic position of this strain. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain NEAU-MES19T was most closely related to Micromonospora matsumotoense IMSNU 22003T. However, phylogenetic analysis based on the gyrB gene sequence showed that the isolate was more closely related to Micromonospora cremea CR30T than M. matsumotoense IMSNU 22003T. The low level of DNA-DNA relatedness allowed the isolate to be differentiated from M. matsumotoense IMSNU 22003T and M. cremea CR30T. Moreover, strain NEAU-MES19T could also be distinguished from its closest phylogenetic relatives by morphological, physiological and biochemical characteristics. Therefore, it is proposed that strain NEAU-MES19T represents a novel species of the genus Micromonospora, for which the name Micromonospora maoerensis sp. nov. is proposed. The type strain is NEAU-MES19T (=CGMCC 4.7091T = DSM 45884T). © 2013 Springer Science+Business Media Dordrecht. Source


Gao R.,Key Laboratory of Agriculture Biological Functional Gene | Liu C.,Key Laboratory of Agriculture Biological Functional Gene | Zhao J.,Key Laboratory of Agriculture Biological Functional Gene | Jia F.,Key Laboratory of Agriculture Biological Functional Gene | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A novel actinomycete, designated strain NEAU-GRX6T, was isolated from mucky soil collected from a stream of Jinlong Mountain in Harbin, Heilongjiang Province, north China, and characterized using a polyphasic approach. The isolate formed irregular sporangia containing motile sporangiospores on the substrate mycelium. The whole-cell sugars were xylose, glucose and galactose. The predominant menaquinones were MK-9(H6), MK-10(H4) and MK-9(H4). The major fatty acids were C16: 0, C15: 0, C18: 1ω9c, C17: 1ω7c and C18: 0. The phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The DNA G+C content was 67 mol%. 16S rRNA gene sequence similarity studies showed that strain NEAU-GRX6T belonged to the genus Actinoplanes, being most closely related to Actinoplanes palleronii IFO 14916T (97.80% similarity) and Actinoplanes missouriensis NBRC 102363T (97.76 %). However, the low observed levels of DNA-DNA relatedness allowed the isolate to be differentiated from the above-mentioned species of the genus Actinoplanes. Moreover, strain NEAU-GRX6T could also be distinguished from A. palleronii IFO 14916T and A. missouriensis NBRC 102363T by phenotypic characteristics. Therefore, it is proposed that strain NEAU-GRX6T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes lutulentus sp. nov. is proposed. The type strain is strain NEAU-GRX6T (=CGMCC 4.7090T=DSM 4=883T). © 2014 IUMS. Source

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