Key Laboratory of Agricultural Animal Genetics

Wuhan, China

Key Laboratory of Agricultural Animal Genetics

Wuhan, China
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Jiao X.-F.,Key Laboratory of Agricultural Animal Genetics | Jiao X.-F.,Huazhong Agricultural University | Huang C.-J.,Key Laboratory of Agricultural Animal Genetics | Huang C.-J.,Huazhong Agricultural University | And 12 more authors.
Oncotarget | Year: 2017

ATP-binding cassette E1 (ABCE1) is a member of the ATP-binding cassette transporters and essential for diverse biological events regulating abroad range of biological functions including viral infection, cell proliferation, anti-apoptosis, translation initiation and ribosome biogenesis. Here, we discovered that Abce1 also plays indispensable roles in mouse oocyte meiotic progression. In the present study, we examined the expression, localization, and function of Abce1 during mouse oocyte meiotic maturation. Immunostaining and confocal microscopy identified that Abce1 localized as small dots in nucleus in germinal vesicle stage. After germinal vesicle breakdown, it dispersedly localized around the whole spindle apparatus. During the anaphase and telophase stages, Abce1 was just like a cap to localize around the two pole region of spindle but not the midbody and chromosome. Knockdown of Abce1 by specific siRNA injection delayed the resumption of meiosis (GVBD) and affected the extrusion of first polar body. Moreover, the process of spindle assembly and chromosome alignment were severely impaired. Abce1-RNAi led to the dissociation of γ-tubulin and p-MAPK from spindle poles, thus caused mounts of spindle morphology abnormities and chromosome alignment defects, leading to high incidence of aneuploidy. Our findings refresh the knowledge of Abce1 function by exploring its role in oocyte meiotic resumption, spindle assembly and chromosome alignment. © Jiao et al.


Lin R.,Key Laboratory of Agricultural Animal Genetics | Zhao C.,Key Laboratory of Agricultural Animal Genetics | Jing L.,Key Laboratory of Agricultural Animal Genetics | Zhao S.,Key Laboratory of Agricultural Animal Genetics | Zhao S.,Huazhong Agricultural University
Cell Biology International | Year: 2015

As a well-known transcription factor, Homeobox A10 (HOXA10) regulates a large number of downstream target genes, leading to the proper function development of endometrium for embryo implantation. The change of HOXA10 gene expression level can alter the expressions of many other genes, including coding and noncoding transcripts. In our study, mRNA and LncRNA expression profiles screening was performed by microarray when the HOXA10 gene expression level increased in Ishikawa cells. A total of 907 mRNAs and 1,026 LncRNAs were identified as differentially expressed transcripts (Fold Change ≥2, P-value <0.05, and Q-value <0.05) between HOXA10 overexpressed and control Ishikawa cells. Further analysis identified that these mRNAs participated in various biological processes, such as blood vessel development, cell adhesion, cell cycle, etc. Also, 14 enhancer-like LncRNAs and 108 LincRNAs with their nearby mRNAs were identified as coregulated transcripts. Our results showed that the mRNA and LncRNA expression profiles differed significantly between the two groups and provided useful information for further studying the molecular mechanisms of HOXA10 in endometrium. © 2015 International Federation for Cell Biology.


Han Y.G.,Key Laboratory of Agricultural Animal Genetics | Liu G.Q.,Key Laboratory of Agricultural Animal Genetics | Liu G.Q.,Huazhong Agricultural University | Jiang X.P.,Key Laboratory of Agricultural Animal Genetics | And 8 more authors.
Molecular Biology Reports | Year: 2013

Ten single nucleotide polymorphisms were used for genotyping of 176 Tongshan Black-boned goats, which are Chinese indigenous goat colony for meat production. The average individual heterozygosity was 0.292. To assess the correlations between individual heterozygosity and growth in Tongshan Black-boned goat individuals, and the potential of using individual heterozygosity as an indicator of growth, the data of growth traits, including body weight, height at withers, body length, chest girth and cannon circumference, were collected. Significant correlations were observed between individual heterozygosity and body weight, height at withers, body length, heart girth, cannon circumference (P<0.05). All the significant regression showed positive slope with R square values ranged from 0.0251 to 0.0368. These data suggests that individual heterozygosity is positively correlated with growth traits in Tongshan Black-boned goat individuals and associative overdominance may affect Tongshan Black-boned goat growth significantly. Therefore it is possible to use individual heterozygosity as an indicator of growth. Our results also provide a strong support to the overdominance hypothesis. © 2013 Springer Science+Business Media Dordrecht.


Tao C.,Key Laboratory of Agricultural Animal Genetics | Zhang Q.,Huazhong Agricultural University | Feng N.,Key Laboratory of Agricultural Animal Genetics | Shi D.,Huazhong Agricultural University | Liu B.,Key Laboratory of Agricultural Animal Genetics
Journal of Dairy Science | Year: 2016

The qualitative and quantitative declaration of food ingredients is important to consumers, especially for genetically modified food as it experiences a rapid increase in sales. In this study, we designed an accurate and rapid detection system using colloidal gold immunochromatographic strip assay (GICA) methods to detect genetically modified cow milk. First, we prepared 2 monoclonal antibodies for human α-lactalbumin (α-LA) and measured their antibody titers; the one with the higher titer was used for further experiments. Then, we found the optimal pH value and protein amount of GICA for detection of pure milk samples. The developed strips successfully detected genetically modified cow milk and non-modified cow milk. To determine the sensitivity of GICA, a quantitative ELISA system was used to determine the exact amount of α-LA, and then genetically modified milk was diluted at different rates to test the sensitivity of GICA; the sensitivity was 10 μg/mL. Our results demonstrated that the applied method was effective to detect human α-LA in cow milk. © 2016 American Dairy Science Association.


Qiao Q.,Huazhong Agricultural University | Qiao Q.,Key Laboratory of Agricultural Animal Genetics | Liang H.,Huazhong Agricultural University | Liang H.,Key Laboratory of Agricultural Animal Genetics | And 2 more authors.
Chemosphere | Year: 2013

Cyanobacterial blooms caused by water eutrophication have become a worldwide problem. Microcystins (MCs) released during cyanobacterial blooms exert toxicity on fish. Up to now, immunotoxicity of MCs on fish has been rarely reported. The present study investigated immune response of crucian carp (Carassius auratus) to cyanobacteria via chronic exposure in diet. Fish were fed with diets containing 20% (low dose group) and 40% (high dose group) of cyanobacteria lyophilized powder. After exposure of 30. d, a batch of assays was determined for assessing immunotoxicity of MCs. The head kidney and spleen indexes significantly increased in high dose group. Blood nitroblue tetrazolium activity in high dose group was nearly twice as much as that in control group with no cyanobacteria additive. Marked haemorrhage and hyperemia were observed in kidney and spleen in high dose group. The edematous mitochondria, deformation of the nucleus and compaction of chromatin occurred in lymphocytes of head kidney and spleen in both cyanobacteria groups. Lysozyme activity showed an obvious increase in low dose group but a sharp decrease in high dose group. Significant increase of macrophage bactericidal activity was detected in low dose group. The present findings indicate that via chronic diet exposure of different cyanobacteria levels, fish exhibit various immune responses. Fish immunity tends to proceed toward the direction of immunostimulative response at low MCs concentrations but toward the trend of immunosuppressive answer at high MCs concentrations. © 2012 Elsevier Ltd.


PubMed | Key Laboratory of Agricultural Animal Genetics
Type: Journal Article | Journal: Molecular biology reports | Year: 2013

Ten single nucleotide polymorphisms were used for genotyping of 176 Tongshan Black-boned goats, which are Chinese indigenous goat colony for meat production. The average individual heterozygosity was 0.292. To assess the correlations between individual heterozygosity and growth in Tongshan Black-boned goat individuals, and the potential of using individual heterozygosity as an indicator of growth, the data of growth traits, including body weight, height at withers, body length, chest girth and cannon circumference, were collected. Significant correlations were observed between individual heterozygosity and body weight, height at withers, body length, heart girth, cannon circumference (P < 0.05). All the significant regression showed positive slope with R square values ranged from 0.0251 to 0.0368. These data suggests that individual heterozygosity is positively correlated with growth traits in Tongshan Black-boned goat individuals and associative overdominance may affect Tongshan Black-boned goat growth significantly. Therefore it is possible to use individual heterozygosity as an indicator of growth. Our results also provide a strong support to the overdominance hypothesis.


PubMed | Key Laboratory of Agricultural Animal Genetics
Type: Journal Article | Journal: Cell biology international | Year: 2015

As a well-known transcription factor, Homeobox A10 (HOXA10) regulates a large number of downstream target genes, leading to the proper function development of endometrium for embryo implantation. The change of HOXA10 gene expression level can alter the expressions of many other genes, including coding and noncoding transcripts. In our study, mRNA and LncRNA expression profiles screening was performed by microarray when the HOXA10 gene expression level increased in Ishikawa cells. A total of 907 mRNAs and 1,026 LncRNAs were identified as differentially expressed transcripts (Fold Change 2, P-value <0.05, and Q-value <0.05) between HOXA10 overexpressed and control Ishikawa cells. Further analysis identified that these mRNAs participated in various biological processes, such as blood vessel development, cell adhesion, cell cycle, etc. Also, 14 enhancer-like LncRNAs and 108 LincRNAs with their nearby mRNAs were identified as coregulated transcripts. Our results showed that the mRNA and LncRNA expression profiles differed significantly between the two groups and provided useful information for further studying the molecular mechanisms of HOXA10 in endometrium.


PubMed | Huazhong Agricultural University and Key Laboratory of Agricultural Animal Genetics
Type: Journal Article | Journal: Journal of dairy science | Year: 2016

The qualitative and quantitative declaration of food ingredients is important to consumers, especially for genetically modified food as it experiences a rapid increase in sales. In this study, we designed an accurate and rapid detection system using colloidal gold immunochromatographic strip assay (GICA) methods to detect genetically modified cow milk. First, we prepared 2 monoclonal antibodies for human -lactalbumin (-LA) and measured their antibody titers; the one with the higher titer was used for further experiments. Then, we found the optimal pH value and protein amount of GICA for detection of pure milk samples. The developed strips successfully detected genetically modified cow milk and non-modified cow milk. To determine the sensitivity of GICA, a quantitative ELISA system was used to determine the exact amount of -LA, and then genetically modified milk was diluted at different rates to test the sensitivity of GICA; the sensitivity was 10 g/mL. Our results demonstrated that the applied method was effective to detect human -LA in cow milk.


PubMed | Key Laboratory of Agricultural Animal Genetics
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

MicroRNAs (miRNAs) play important roles in the development and biochemical functions of skeletal muscles. However, targeting of miRNAs to structural genes involved in Z-discs have not been investigated. Here, we describe a highly expressed miRNA, miR-432, in pig embryonic skeletal muscle, which appeared to target myozenin1 (MYOZ1), a protein involved in the muscular sarcomere microstructure. Our results showed that miR-432 is involved in muscle development in the developing pig. In addition, it promoted differentiation of the C2C12 myoblast cell line into myotubes. We also demonstrated that inhibition of miR-432 reduced proliferation of C2C12 cells, suggesting that miR-432 is involved in regulation of myoblast proliferation. Moreover, molecular markers of muscle differentiation and fiber type (Myh7/ slow and Myh4/ fast IIB) showed that miR-432 reduced the differentiation rate of C2C12 cells. These results provide insights into the potential functions of miR-432 as well as its proposed target, MYOZ1, during muscle development. This may lead to applications for further improvements in porcine muscle growth, and may enhance our understanding of complex inherited human muscle disorders.

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