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Lu K.,Guizhou University | Huang Y.,Guizhou University | Wu J.,Guizhou University | Zhu Q.,Guizhou University | Zhu Q.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province
Advanced Engineering and Technology - Proceedings of the 2014 Annual Congress on Advanced Engineering and Technology, CAET 2014 | Year: 2014

Active Packaging membrane with slow-release function was produced by allyl isothiocyanate molecularly imprinted polymer (AITC-MIPs) cooperating chitosan (AITC-MIPs-co-CS). The preservation effects of the membrane were investigated according to the physicochemical indexes on chilling meat stored at 4°C. At the same time, unpackaged group, pure chitosan coated membrane group and chitosan membrane including AITC inclusion complex group were set as the control samples. Preservation effects of AITC-MIPs-co-CS on chilled meat is superior to the other control groups, which can effectively inhibit the growth of bacteria and delay the rise of volatile base nitrogen (TVB-N), sulfur generation barbituric acid reagent (TBARS) and pH. After 11 d storage, pH is 6.48, TVB-N 15.12 mg/100 g, and total number of bacteria 4.52lg CUF/g. The storage period is 3.67 times for the group 3 as long as the CK group. It can significantly prolong the shelf life of chilled meat by a packaging membrane of AITC-MIPs-co-CS. © 2014 Taylor & Francis Group. Source


Huang Y.,Guizhou University | Zhu Q.,Guizhou University | Zhu Q.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province
Journal of Chemistry | Year: 2015

This paper theoretically investigates interactions between a template and functional monomer required for synthesizing an efficient molecularly imprinted polymer (MIP). We employed density functional theory (DFT) to compute geometry, single-point energy, and binding energy (Δ E) of an MIP system, where spermidine (SPD) and methacrylic acid (MAA) were selected as template and functional monomer, respectively. The geometry was calculated by using B3LYP method with 6-31+(d) basis set. Furthermore, 6-311++(d, p) basis set was used to compute the single-point energy of the above geometry. The optimized geometries at different template to functional monomer molar ratios, mode of bonding between template and functional monomer, changes in charge on natural bond orbital (NBO), and binding energy were analyzed. The simulation results show that SPD and MAA form a stable complex via hydrogen bonding. At 1: 5 SPD to MAA ratio, the binding energy is minimum, while the amount of transferred charge between the molecules is maximum; SPD and MAA form a stable complex at 1: 5 molar ratio through six hydrogen bonds. Optimizing structure of template-functional monomer complex, through computational modeling prior synthesis, significantly contributes towards choosing a suitable pair of template-functional monomer that yields an efficient MIP with high specificity and selectivity. © 2015 Yujie Huang and Qiujin Zhu. Source


Su W.,Guizhou University | Su W.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province | Tang S.,Guizhou University | Xie C.,Guizhou University | And 5 more authors.
International Journal of Food Properties | Year: 2016

Antioxidant and DNA damage protection activity of duck gizzard peptides were evaluated using chemiluminescence method. The duck gizzard peptides exhibited free radical scavenging activity against superoxide anions, hydroxide radicals, and hydrogen peroxide, as well as DNA damage protection in a dose-dependent manner. The duck gizzard peptides had varying degrees of antioxidant activity for the inhibition of different radical species as well as DNA damage, with half-inhibition concentration (IC50) values of 14.58, 0.0163, 19.80, and 1.93 mg/mL, respectively. The duck gizzard peptides had the strongest inhibition of hydroxyl radical activity, and the weakest inhibition of hydrogen peroxide radicals. The results clearly indicated that duck gizzard peptides were good sources of natural antioxidants for future applications in the food industry. Copyright © Taylor & Francis Group, LLC. Source


Zhang R.,Guizhou University | Zhang R.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province | He L.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province | He L.,Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy | And 5 more authors.
Korean Journal for Food Science of Animal Resources | Year: 2016

Cardiovascular and cerebrovascular diseases seriously harm human health, and Bifidobacterium is the most beneficial probiotic in the gastrointestinal tract of humans. This work aimed to screen cholesterol-lowering Bifidobacterium from Guizhou Xiang Pig and evaluate its tolerance to oxygen, acid, and bile. Twenty-seven aerotolerant strains with similar colony to Bifidobacterium were isolated through incubation at 37°C in 20% (v/v) CO2-80% (v/v) atmospheric air by using Mupirocin lithium modified MRS agar medium, modified PTYG with added CaCO3, and modified PTYG supplemented with X-gal. Ten strains with cholesterol-lowering rates above 20% (w/w) were used for further screening. The selected strains' tolerance to acid and bile was then determined. A combination of colony and cell morphology, physiological, and biochemical experiments, as well as 16S rRNA gene-sequence analysis, was performed. Results suggested that BZ25 with excellent characteristics of high cholesterol-removal rate of 36.32% (w/w), as well as tolerance to acid and bile, was identified as Bifidobacterium animalis subsp. lactis. To further evaluate Bifidobacterium BZ25's growth characteristic and tolerance to oxygen, culture experiments were performed in liquid medium and an agar plate. Findings suggested that BZ25 grew well both in environmental 20% (v/v) CO2-80% (v/v) atmospheric air and in 100% atmospheric air because BZ25 reached an absorbance of 1.185 at 600 nm in 100% atmospheric air. Moreover, BZ25 was aerotolerant and can grow in an agar medium under the environmental condition of 100% atmospheric air. This study can lay a preliminary foundation for the potential industrial applications of BZ25. © 2016 Korean Society for Food Science of Animal Recources. Source


Ding Z.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province | Wang Z.,Zunyi Medical College | Zheng W.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province | Liu H.,Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province
Journal of Chinese Institute of Food Science and Technology | Year: 2014

In order to investigate the effect of different packaging on volatile flavor compounds of dried peppers and provide a scientific basis to improveof dried peppers flavor during storage, the flavor compounds in dried peppers with differecce kinds of packaging were extracted by solid-phase microextraction (SPME). Analysis was carried out by gas chromatography-mass spectrometry (GC-MS). The result showed that the flavor quality of treatments was declined during storage. The synthetical score of the control was the highest of 4.90003, the second score of the PE film atmospheric packaging was 2.863883, and the lowest score of the sample of PET vacuum packaging was -1.06115. The total synthetical score of different packaging material samples was in the order: PE, PEX, PET; craft (P<0.05). The total synthetical score of different packaging method samples was in the order: atmospheric packaging, vacuum packaging, deoxidizer vacuum packaging, desiccant vacuum packaging (P>0.05). The kinds and relative content of volatile flavor components was significant differences among difference packaging samples. The qualities of packaging materials was the important factors of the stability of flavor quality. Packaging material had significantl influence on the flavour quality. The flavor quality of PE film atmospheric packaging had best effect. Source

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