Key Laboratory for Tobacco Gene Resources

Qingdao, China

Key Laboratory for Tobacco Gene Resources

Qingdao, China
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Wang D.,Chinese Academy of Agricultural Sciences | Wang D.,Key Laboratory for Tobacco Gene Resources | Wang S.,Chinese Academy of Agricultural Sciences | Chao J.,Chinese Academy of Agricultural Sciences | And 15 more authors.
Planta | Year: 2017

Main conclusion: A novel tobacco mutant library was constructed, screened, and characterized as a crucial genetic resource for functional genomics and applied research.A comprehensive mutant library is a fundamental resource for investigating gene functions, especially after the completion of genome sequencing. A new tobacco mutant population induced by ethyl methane sulfonate mutagenesis was developed for functional genomics applications. We isolated 1607 mutant lines and 8610 mutant plants with altered morphological phenotypes from 5513 independent M2 families that consisted of 69,531 M2 plants. The 2196 mutations of abnormal phenotypes in the M2 putative mutants were classified into four groups with 17 major categories and 51 subcategories. More than 60% of the abnormal phenotypes observed fell within the five major categories including plant height, leaf shape, leaf surface, leaf color, and flowering time. The 465 M2 mutants exhibited multiple phenotypes, and 1054 of the 2196 mutations were pleiotropic. Verification of the phenotypes in advanced generations indicated that 70.63% of the M3 lines, 84.87% of the M4 lines, and 95.75% of the M5 lines could transmit original mutant phenotypes of the corresponding M2, M3, and M4 mutant plants. Along with the increased generation of mutants, the ratios of lines inheriting OMPs increased and lines with emerging novel mutant phenotypes decreased. Genetic analyses of 18 stably heritable mutants showed that two mutants were double recessive, five were monogenic recessive, eight presented monogenic dominant inheritance, and three presented semi-dominant inheritance. The pleiotropy pattern, saturability evaluation, research prospects of genome, and phenome of the mutant populations were also discussed. Simultaneously, this novel mutant library provided a fundamental resource for investigating gene functions in tobacco. © 2017 Springer-Verlag Berlin Heidelberg


Wu Q.,Chinese Academy of Agricultural Sciences | Wu Q.,Key Laboratory for Tobacco Gene Resources | Wu X.,Chinese Academy of Agricultural Sciences | Wu X.,Key Laboratory for Tobacco Gene Resources | And 11 more authors.
Molecular Breeding | Year: 2014

Leaf color is an indicator of chlorophyll (Chl) level, and isolating leaf color mutants can facilitate the understanding of Chl metabolism regulation. Here, we describe an ethyl methanesulfonate-induced light color mutant white stem 1 (ws1) in common tobacco (Nicotiana tabacum L.) that shows a phenotype highly similar to burley tobacco (Nicotiana tabacum L.), a type of air-cured tobacco that has light-colored leaves with white veins. Compared with the wild type, the light green stem of ws1 gradually became pale white along with growth, while ws1 leaves lost green color rapidly, which was positively correlated with the decline of Chl levels. A series of genetic analyses indicated that the ws1 mutant phenotype was controlled by two recessive nuclear genes ws1a and ws1b which were preliminarily mapped to the intervals of tobacco simple sequence repeat markers linkage groups 5 and 24 using the BC1F2 populations, respectively. The allelism test further revealed that the same two genes controlled the burley character in burley tobacco. Based on the Chl-deficient phenotype of ws1 and the locations of the two genes, we hypothesized that ws1a and ws1b were paralogs of each other probably originated from the ancestral species N. sylvestris and N. tomentosiformis, respectively. Both genes might share similar biological functions and expression patterns, and play key roles in the regulation of Chl biosynthesis. These results laid a solid foundation for marker-assisted selection breeding and gene function analysis of the burley character in tobacco. © 2014, Springer Science+Business Media Dordrecht.

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