Key Laboratory for Pesticide Residue Detection

Hangzhou, China

Key Laboratory for Pesticide Residue Detection

Hangzhou, China
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Yuan Y.,Zhejiang Academy of Agricultural Sciences | Yuan Y.,Key Laboratory for Pesticide Residue Detection | Hu G.,Zhejiang Academy of Agricultural Sciences | Hu G.,Key Laboratory for Pesticide Residue Detection | And 14 more authors.
Journal of Agricultural and Food Chemistry | Year: 2016

Multielement and stable isotope (δ13C, δ15N, δ2H, δ18O, 207Pb/206Pb, and 208Pb/206Pb) analyses were combined to provide a new chemometric approach to improve the discrimination between organic and conventional Brassica vegetable production. Different combinations of organic and conventional fertilizer treatments were used to demonstrate this authentication approach using Brassica chinensis planted in experimental test pots. Stable isotope analyses (δ15N and δ13C) of B. chinensis using elemental analyzer-isotope ratio mass spectrometry easily distinguished organic and chemical fertilizer treatments. However, for low-level application fertilizer treatments, this dual isotope approach became indistinguishable over time. Using a chemometric approach (combined isotope and elemental approach), organic and chemical fertilizer mixes and low-level applications of synthetic and organic fertilizers were detectable in B. chinensis and their associated soils, improving the detection limit beyond the capacity of individual isotopes or elemental characterization. LDA shows strong promise as an improved method to discriminate genuine organic Brassica vegetables from produce treated with chemical fertilizers and could be used as a robust test for organic produce authentication. © 2016 American Chemical Society.


Yuan Y.,Zhejiang Academy of Agricultural Sciences | Yuan Y.,Key Laboratory for Pesticide Residue Detection | Hu G.,Zhejiang Academy of Agricultural Sciences | Hu G.,Key Laboratory for Pesticide Residue Detection | And 8 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

To further investigate the method of using δ15N as a marker for organic vegetable discrimination, the effects of different fertilizers on the δ15N in different growing stages of Brassica chinensis (B. chinensis) grown in uncultivated soil were investigated with a pot experiment. B. chinensis was planted with uncultivated soil and different fertilizer treatments and then harvested three times in three seasons consecutively. For the spring experiments in the years of 2011 and 2012, the δ15N value of B. chinensis, which increased due to organic manure application and decreased due to chemical fertilizer application, was significantly different (p < 0.05) with manure treatment and chemical treatment. The δ15N value of vegetables varied among three growing stages and ranged from +8.6‰ to +11.5‰ for the control, from +8.6‰ to +12.8‰ for the compost chicken manure treatment, from +2.8‰ to +7.7‰ for the chemical fertilizer urea treatment, and from +7.7‰ to +10.9‰ for the compost-chemical fertilizer treatment. However, the δ15N values observed in the autumn experiment of 2011 without any fertilizer application increased ranging from +13.4‰ to +15.4‰, + 11.2‰ to +17.7‰, +10.7‰ to +17.1‰, and +10.6‰ to +19.1‰, respectively, for the same treatments mentioned above. This result was not significantly different between manure treatment and chemical treatment. The δ15N values of soil obtained in the spring of 2011 during three growing stages were slightly affected by fertilizers and varied in the range of +1.6‰ to +2.5‰ for CK, +4.7‰ to +6.5‰ for compost treatment, +2.1‰ to +2.4‰ for chemical treatment, and +2.7‰ to +4.6‰ for chemical-compost treatment, respectively. High δ15N values of B. chinensis were observed in these experiments, which would be useful to supplement a δ15N database for discriminating organic vegetables. Although there was a significant difference between manure treatment and chemical treatment, it was still difficult to discriminate whether a labeled organic vegetable was really grown without chemical fertilizer just with a fixed high δ15N value, especially for the vegetables planted simultaneously with chemical and compost fertilizer. © 2014 American Chemical Society.

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