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Wu T.,Beihang University | Wang H.,Beihang University | Huang S.,Peking Union Medical College | Ye X.,Mount Sinai School of Medicine | And 5 more authors.
Human Genetics | Year: 2010

Although multiple genes have been identified as genetic risk factors for isolated, non-syndromic cleft lip with/without cleft palate (CL/P), a complex and heterogeneous birth defect, interferon regulatory factor 6 gene (IRF6) is one of the best documented genetic risk factors. In this study, we tested for association between markers in IRF6 and CL/P in 326 Chinese case-parent trios, considering gene-environment interaction for two common maternal exposures, and parent-of-origin effects. CL/P case-parent trios from three sites in mainland China and Taiwan were genotyped for 22 single nucleotide polymorphisms (SNPs) in IRF6. The transmission disequilibrium test was used to test for marginal effects of individual SNPs. We used PBAT to screen the SNPs and haplotypes for gene-environment (G × E) interaction and conditional logistic regression models to quantify effect sizes for SNP-environment interaction. After Bonferroni correction, 14 SNPs showed statistically significant association with CL/P. Evidence of G × E interaction was found for both maternal exposures, multivitamin supplementation and environmental tobacco smoke (ETS). Two SNPs showed evidence of interaction with multivitamin supplementation in conditional logistic regression models (rs2076153 nominal P = 0.019, rs17015218 nominal P = 0.012). In addition, rs1044516 yielded evidence for interaction with maternal ETS (nominal P = 0.041). Haplotype analysis using PBAT also suggested interaction between SNPs in IRF6 and both multivitamin supplementation and ETS. However, no evidence for maternal genotypic effects or significant parent-of-origin effects was seen in these data. These results suggest IRF6 gene may influence risk of CL/P through interaction with multivitamin supplementation and ETS in the Chinese population. © 2010 Springer-Verlag.

Yang S.,Key Laboratory for Oral Biomedical Engineering | Yang S.,Hainan Medical College | Zhang J.,Hubei University | Chen X.,Key Laboratory for Oral Biomedical Engineering | Chen X.,Hubei University
International Journal of Oral and Maxillofacial Surgery | Year: 2010

Myoepithelial carcinoma with predominantly clear cell morphology is rare. A review of the literature identified 15 unequivocal cases, only two of which were of minor salivary gland origin. A case of minor salivary gland clear cell myoepithelial carcinoma of the retromolar region in a 70-year-old man is presented. It is important to recognize the clinicopathologic features of this unusual tumor, because of its histological similarity to several other primary and metastatic clear cell tumors and its aggressive behavior. © 2009 International Association of Oral and Maxillofacial Surgeons.

Zhu Q.,Key Laboratory for Oral Biomedical Engineering | Li J.,Key Laboratory for Oral Biomedical Engineering | Li J.,Wuhan University
Medical Hypotheses | Year: 2011

The prevalence of cleft lip with or without cleft palate (CL/P) at birth varied from 3.4-22.9 per 10. 000 births according to different geography and ethnic groups. And scar has been recognized as a significantly negative factor on the postoperative wound healing of cleft lip patients because it often impairs function, blocks growth and makes the face aesthetically unpleasant. TGF-β (Transforming growth factor-β), a chemotactic factor of monocytes, fibroblasts and macrophages, which stimulates collagen and other extracellular matrix deposition by fibroblasts, could promote angiogenesis and stimulate the scar formation, which hinder the aesthetic effects on the face. Theoretically, inhibitors of TGF-β could inhibit scar formation, which could be a potential application for the therapy of cleft lip patients. Therefore a combined therapy based on inhibitors of TGF-β may be taken as an important treatment to control postoperative scar in the future. © 2010 Elsevier Ltd.

Chen F.,Key Laboratory for Oral Biomedical Engineering | Wu T.,Key Laboratory for Oral Biomedical Engineering | Cheng X.,Wuhan University
Gerodontology | Year: 2014

Background To date, there have been very little data on the cytotoxic responses of different cell lines to denture adhesives. Objective To determine the cytotoxicity of three denture adhesives on primary human oral keratinocytes (HOKs), fibroblasts (HOFs) and permanent mouse fibroblasts cell lines (L929). Methods Three commercial denture adhesives (two creams and one powder) were prepared for indirect contact using the agar diffusion test, as well as extracts in MTT assay. The results of the MTT assay were statistically analysed by one-way anova and Tukey's test (p < 0.05). Results All of the tested denture adhesives showed mild to moderate cytotoxicity to primary HOKs (p < 0.001), whereas none of three was toxic to L929 cells (p > 0.05) in both assays. For primary HOFs cultures, slight cytotoxicity was observed for one of the products from the agar diffusion test and undiluted eluates of all tested adhesives with MTT assay (p < 0.01). Conclusion Denture adhesives are toxic to the primary HOKs and HOFs cultures, whereas non-toxic to L929 cells. The results suggest that primary human oral mucosal cells may provide more valuable information in toxicity screening of denture adhesives. © 2012 John Wiley & Sons A/S and The Gerodontology Association. Published by John Wiley & Sons Ltd.

Zhu W.C.,Key Laboratory for Oral Biomedical Engineering | Zhu W.C.,North Sichuan Medical College | Xiao J.,Key Laboratory for Oral Biomedical Engineering | Liu Y.,Key Laboratory for Oral Biomedical Engineering | And 2 more authors.
Cleft Palate-Craniofacial Journal | Year: 2010

Objective: To determine the levels of dental caries in subjects with cleft lip and/or palate in China in comparison with controls. Design: A total of 380 subjects with cleft lip and/or palate and 339 noncleft control subjects were examined for dental caries using the decayed-missingfilled-teeth (DMFT/dmft) and decayed-missing-filled-surfaces (DMFS/dmfs) index. A questionnaire was used to acquire subjects' living habits and family socioeconomic status. Setting: West China Stomatology Hospital, Sichuan University. Subjects: Individuals between 3 and 25 years of age. Results: Caries prevalence and scores for dmft/DMFT and dmfs/DMFS were significantly higher in subjects with cleft palates when compared with the noncleft control group (p , .05), except 3- to 5-year-old children (p . .05). However, in the 3- to 5-year-old group, children with cleft lip and/or palate had significantly more caries than children of the same age with only a cleft lip with or without alveolus (p , .05). Children whose clefts had been surgically repaired had a lower dmft and dmfs than those whose clefts had not been surgically repaired (p , .05). Conclusion: Individuals with oral cleft are at an increased risk for dental caries. The types of cleft and the surgical repair are two important factors for dental caries.

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