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Li Z.,Chinese Academy of Sciences | Li Z.,Key Laboratory for Oil Crops Genetic Improvement | Lu G.Y.,Chinese Academy of Sciences | Lu G.Y.,Key Laboratory for Oil Crops Genetic Improvement | And 8 more authors.
Seed Science and Technology | Year: 2010

In this study, the application of gibberellic acid-3 (GA3) during priming to improve drought tolerance in rapeseed (Brassica napus L.) was evaluated. Seeds of rapeseed (c.v. Zhongshuang No. 10) were primed in 0, 100, 200, 300, 400 and 500 mgl-1 aerated solution of GA3 for 8 h. The primed seeds were set to germinate for 7 days under simulated drought stress by applying 15% PEG6000 solution. GA3 significantly improved seed germination characteristics under drought stress. Compared with no-GA 3 priming treatment, seeds with 300 mg1-1 GA3 showed a significantly increased drought tolerance index (88% increase), seedling fresh weight (42.9%) and hypocotyl length (72.7%). GA3 treatment also significantly increased soluble sugars (47.2% increase), soluble proteins (35.6%) and free proline (62.9%). The level of electrolyte leakage from seedling cotyledons was significantly reduced after priming and the malondialdehyde (MDA) content of the cotyledons decreased by 36.6%, possibly due to the enHanced levels of superoxide dismutase (increased by 13%), catalase (increased by 45.6%) and peroxidase (increased by 46.6%). The result of this research showed that use of GA3 during priming enHances seed germination of rapeseed and improves seedling tolerance to drought stress.

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