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Zhang Y.,Northwest University, China | Yang L.J.,Northwest University, China | Wang X.X.,Northwest University, China | Ma X.Y.,Northwest University, China | And 6 more authors.
Academic Journal of Second Military Medical University | Year: 2014

Objective: To investigate the effect of rhizome cyperi on pharmacokinetics of ferulic acid in ligusticum wallichii in normal and nitroglycerin migraine model rats. Methods: The migraine model were made by nitroglycerin, SD rats were divided into normal rhizoma ligustici wallichii group  normal rhizoma ligustici wallichii and rhizome cyperi group  Model rhizoma ligustici wallichii group  Model rhizoma ligustici wallichii and rhizome cyperi group.We used RP-HPLC 0.2% formic acid water (A)-methanol (B) gradient elution 322nm to detect the concentration of FA in the plasma of rats blood at 8h after orally administrated and we used DAS2.0 pharmacokinetic calculation software to calculate the pharmacokinetic parameters.Results: Inspection conform to the requirements of the determination of the methodology. In normal groups, compared with ligusticum wallichii decoction group, the absorption half time t1/2Ka of FA was significantly reduced(P<0.05): the peak time (tmax) was advanced, however, there was no difference in other parameters. In model groups, the maximum plasma concentration (Cmax),area under curve (AUC), distribution half-life time (t1/2α), and apparent volume of distribution(V1/F) of FA were increased significantly(P<0.01); the peak time was advanced, the absorption rate and the clearance speed (CL/F) was reduced (P<0.01). Conclusion: Rhizoma cyperi could enhance the absorption of FA in normal rats, while Rhizoma cyperi reduced the distribution and metabolism, increased the bioavailability of the FA in model rats. © 2014, Second Military Medical University Press. All rights reserved.

Meng X.,Northwest University, China | Zhao X.,Northwest University, China | Wang S.,Northwest University, China | Jia P.,Northwest University, China | And 4 more authors.
Journal of Analytical Methods in Chemistry | Year: 2013

A sensitive and specific gas chromatographic-mass spectrometry with selected ion monitoring (GC-MS/SIM) method has been developed for simultaneous identification and quantification of α-asarone, β -asarone, and methyl eugenol of Acorus tatarinowii Schott in rat plasma. Chromatographic separation was performed on a Restek Rxi-5MS capillary column (30 m × 0.32 mm × 0.25 m), using 1-naphthol as internal standard (IS). MS detection of these compounds and IS was performed at m/z 178, 208, 208, and 144. Intra- and interday precisions of all compounds of interest were less than 10%. The recoveries are situated in the range of 92.4-105.2%. Pharmacokinetics of methyl eugenol confirmed to be one-compartment open model, α-asarone and β-asarone was two-compartment open model, respectively. The method will probably be an alternative to simultaneous determination and pharmacokinetic study of volatile ingredients in Acorus tatarinowii Schott. © 2013 Xue Meng et al.

Meng X.,Northwest University, China | Liao S.,Northwest University, China | Wang X.,Key Laboratory for New Drugs Research of TCM in Shenzhen | Wang S.,Northwest University, China | And 6 more authors.
Biotechnology Letters | Year: 2014

P-Glycoprotein (P-gp), an ATP-binding cassette transporter, plays an important role in multidrug resistance (MDR). α-Asarone and β-asarone, bioactive cis-trans isomers found in Acorus tatarinowii Schott, were tested for their potential ability to modulate the expression and function of P-gp in Caco-2 cells. MTT assays revealed that both α-asarone and β-asarone significantly enhanced the vincristine-induced cytotoxicity to cells. β-Asarone was the most potent. Flow cytometry showed that α- and β-asarone increased Rhodamine 123 (Rh123) uptake and inhibited Rh123 efflux in Caco-2 cells in a concentration-dependent manner. Furthermore, P-gp expression and P-gp mRNA in cells were decreased by exposure to α- and β-asarone. In addition, β-asarone increased the inhibition of P-gp activity in cells more than α-asarone. Thus, α- and β-asarone effectively reversed MDR by inhibiting P-gp function and expression. © 2013 Springer Science+Business Media Dordrecht.

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