The Key Laboratory for Conservation Biology of Endangered Wildlife

Chengdu, China

The Key Laboratory for Conservation Biology of Endangered Wildlife

Chengdu, China
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Yu F.,Zhejiang University | Qian X.,Zhejiang University | Zeng Z.,Zhejiang University | Zhao X.,Zhejiang University | And 6 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2017

This study aims to investigate the effects of different concentrations of antioxidant of bamboo leaves (AOB) on mouse embryonic stem (mES) cells and to explore the underlying molecular mechanism by which AOB affects the proliferation and apoptosis of mES cells. Compared with the control group, Oct4 expression was remarkably upregulated and Nanog expression was downregulated in mES cells after treatment with AOB at 50 μg/ml, while both the expressions of Oct4 and Nanog were significantly upregulated in mES cells after treatment with AOB at 100 μg/ml. AOB exposure remarkably increased the level of p-ERK (P < 0.01), whereas treatment with ERK pathway inhibitor U0126 markedly depressed the level of p-ERK in AOB-treated embryoid bodies (mEBs) (P < 0.01). Moreover, the levels of β-catenin, SOX17, CABYR, and P450scc were significantly increased following AOB treatment, but decreased in the treatment with AOB+U0126 or AOB+DKK1 (Wnt pathway inhibitor) (P < 0.01). In conclusion, our data indicated that AOB had embryo-toxicity which might be mediated by activation of ERK and Wnt pathway. © 2017, E-Century Publishing Corporation. All rights reserved.

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