Liu Y.,Beijing Key Laboratory of Engineered Drug |
Liu Y.,Key Laboratory for Cell Proliferation |
Liu Y.,Beijing Normal University |
Zhao C.,Key Laboratory for Cell Proliferation |
And 11 more authors.
Canadian Journal of Physiology and Pharmacology | Year: 2010
4-Demethylepipodophyllotoxin 7′-O-β-D-glucopyranoside (4′ DPG), a new podophyllotoxin glucoside with a chemical structure similar to that of 4-demethylpicropodophyllotoxin 7′-O-β-D-glucopyranoside (4DPG), was isolated from the rhizomes of Sinopodophyllum emodi (Wall.) Ying (Berberidaceae). Like 4DPG and etoposide (VP-16), 4′ DPG displayed dose- and time-dependent cytotoxic effects in HepG2 cells. Flow cytometric analysis demonstrated the presence of apoptotic cells and DNA fragmentation after HepG2 cells were exposed to 4′ DPG (1 μmol/L). In addition, 4′ DPG-driven apoptotic events were associated with upregulation of Bax and downregulation of Bcl-2. These results suggest that 4′ DPG has prominent cytotoxic activity and induces apoptosis by increasing the ratio of Bax to Bcl-2 in HepG2 cells.