Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education

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Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education

Laboratory for, China

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Li X.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Wang Z.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Zhao T.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Fan Y.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | And 2 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2016

This study provided a new method to in vitro evaluate the biocompatibility of nanoscaled scaffolds for tissue engineering with neutrophils other than ordinary cell culture. The neutrophils were separated from human peripheral blood of healthy subjects. In vitro degradation product of nanohydroxyapatite/collagen (nHAC), nanohydroxyapatite/collagen/poly (L-lactic acid) (nHACP), and nHACP reinforced by chitin fibers (nHACP/CF) in the D-Hank's Balanced Salt Solution (D-HBSS) was used as the testing solution, which was thereafter mixed with the neutrophils. It was shown that the cell survival rate in the testing solutions had no significant difference from that in the D-HBSS (control). However, from both gene and protein expression levels, the lactate dehydrogenase and tumor necrosis factor-alpha of the neutrophils in the nHACP/CF testing solution were found lowest during the whole testing period; the main reasons of which might be that the calcium release rate of the scaffold was slowest and that the pH value of its degradation solution was nearest to that of human body. Moreover, in vivo experiments showed that most inflammation reactions happened for nHAC and poly (L-lactic acid) groups, while the least inflammation reactions happened for nHACP/CF group in the subcutaneous dorsum of mice at 2 weeks after the surgery, which confirmed the in vitro findings. These results indicated that the pH value and the certain metal iron concentration of the nanoscaled scaffold degradation solution should be two important factors that significantly affect its biocompatibility. This study provides a simple and effective biocompatibility test method for biodegradable nanoscaled tissue engineering scaffolds. © 2016 Wiley Periodicals, Inc.


Zou T.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Liu H.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education
Journal of Biomedical Materials Research - Part A | Year: 2016

Vascular regeneration is known to play an essential role in the repair of injured tissues mainly through accelerating the repair of vascular injury caused by vascular diseases, as well as the recovery of ischemic tissues. However, the clinical vascular regeneration is still challenging. Cell-based therapy is thought to be a promising strategy for vascular regeneration, since various cells have been identified to exert important influences on the process of vascular regeneration such as the enhanced endothelium formation on the surface of vascular grafts, and the induction of vessel-like network formation in the ischemic tissues. Here are a vast number of diverse cell-based strategies that have been extensively studied in vascular regeneration. These strategies can be further classified into three main categories, including cell transplantation, construction of tissue-engineered grafts, and surface modification of scaffolds. Cells used in these strategies mainly refer to terminally differentiated vascular cells, pluripotent stem cells, multipotent stem cells, and unipotent stem cells. The aim of this review is to summarize the reported research advances on the application of various cells for vascular regeneration, yielding insights into future clinical treatment for injured tissue/organ. © 2016 Wiley Periodicals, Inc.


Zhao T.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Fan Y.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Feng Q.,State Key Laboratory of New Ceramic and Fine ProcessingTsinghua UniversityBeijing100084 China | Cui F.-z.,State Key Laboratory of New Ceramic and Fine ProcessingTsinghua UniversityBeijing100084 China | Li X.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education
Journal of Biomedical Materials Research - Part A | Year: 2015

Bone regeneration is a complicated process that involves a series of biological events, such as cellular recruitment, proliferation and differentiation, and so forth, which have been found to be significantly affected by controlled drug delivery. Recently, a lot of research studies have been launched on the application of nanomaterials in controlled drug delivery for bone regeneration. In this article, the latest research progress in this area regarding the use of bioceramics-based, polymer-based, metallic oxide-based and other types of nanomaterials in controlled drug delivery for bone regeneration are reviewed and discussed, which indicates that the controlling drug delivery with nanomaterials should be a very promising treatment in orthopedics. Furthermore, some new challenges about the future research on the application of nanomaterials in controlled drug delivery for bone regeneration are described in the conclusion and perspectives part. © 2015 Wiley Periodicals, Inc.


Li X.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Zhao T.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Sun L.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Fan Y.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | And 2 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2015

As their name suggests, conductive nanomaterials (CNMs) are a type of functional materials, which not only have a high surface area to volume ratio, but also possess excellent conductivity. Thus far, CNMs have been widely used in biomedical applications, such as effectively transferring electrical signals, and providing a large surface area to adsorb proteins and induce cellular functions. Recent works propose further applications of CNMs in biosensors, tissue engineering, neural probes, and drug delivery. This review focuses on common types of CNMs and elaborates on their unique properties, which indicate that such CNMs have a potential to develop into a class of indispensable biomaterials for the diagnosis and therapy of human diseases. © 2015 Wiley Periodicals, Inc.


Wang D.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Liu H.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education
Microscopy Research and Technique | Year: 2015

Cardiovascular disease is the primary cause of morbidity and mortality in today's world. Due to the lack of healthy autologous vessels, more tissue-engineered blood vessels are needed to repair or replace the damaged arteries. Biomaterials play an indispensable role in creating a living neovessel with biological responses. Silk fibroin produced by silkworms possesses good cytocompatibility, tailorable biodegradability, suitable mechanical properties, and minimal inflammatory reactions. In addition, regenerated silk fibroin solutions can be processed into various forms of scaffolds such as films, fibers, tubes, and porous sponges. These features make silk fibroin a promising biomaterial for small-diameter vascular grafts. The present article focuses on the applications of silk fibroin for vascular regeneration. A brief overview of the properties of silk fibroin is provided, following which the current research status and future directions of the main types of silk fibroin scaffolds for vascular regeneration are reviewed and discussed. © 2015 Wiley Periodicals, Inc.


Gong H.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Gong H.,Beihang University | Gong H.,Hong Kong Polytechnic University | Zhang M.,Hong Kong Polytechnic University | Fan Y.,Beihang University
Journal of Mechanics in Medicine and Biology | Year: 2011

Bone tissue material nonlinearity and large deformations within the trabecular network are important for the characterization of failure behavior of trabecular bone at both the apparent and tissue levels. Micro-finite element analysis (μFEA) is a useful tool for determining the mechanical properties of trabecular bone due to certain experimental difficulties. The aim of this study was to determine the effects of bone tissue nonlinear material properties on the apparent- and tissue-level mechanical parameters of trabecular bone using μFEA. A bilinear tissue constitutive model was proposed to describe the bone tissue material nonlinearity. Two trabecular specimens with different micro-architectures were taken as examples. The effects of four parameters, i.e., tissue Young's modulus, tissue yield strain in tension, tissue yield strain in compression, and post-yield modulus on the apparent yield stress/strain, tissue von Mises stress distribution, the amount of tissue elements yielded in compression and tension under compressive and tensile loading conditions were obtained using nine cases for different values of those parameters by totally 36 nonlinear μFEA. These data may provide a reference for more sophisticated evaluations of bone strength and the related fracture risk. © 2011 World Scientific Publishing Company.


Liu M.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Liu M.,Beihang University | Song W.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Song W.,Beihang University | And 14 more authors.
PLoS ONE | Year: 2013

The neuropeptide galanin and its receptors (GalR) are found to be up-regulated in brains suffering from nerve injury, but the specific role played by galanin remains unclear. This study aimed to explore the neuroprotective role of galanin after shear stress induced nerve injury in the primary cultured cortical neurons of rats. Our results demonstrated that no significant changes in cell death and viability were found after galanin treatment when subjected to a shear stress of 5 dyn/cm2 for 12 h, after increasing magnitude of shear stress to 10 dyn/cm2 for 12 h, cell death was significantly increased, while galanin can inhibit the nerve injury induced by shear stress with 10 dyn/cm2 for 12 h. Moreover, Gal2-11 (an agonist of GalR2/3) could also effectively inhibit shear stress-induced nerve injury of primary cultured cortical neurons in rats. Although GalR2 is involved in the galanin protection mechanism, there was no GalR3 expression in this system. Moreover, galanin increased the excitatory postsynaptic currents (EPSCs), which can effectively inhibit the physiological effects of shear stress. Galanin was also found to inhibit the activation of p53 and Bax, and further reversed the down regulation of Bcl-2 induced by shear stress. Our results strongly demonstrated that galanin plays a neuroprotective role in injured cortical neurons of rats. © 2013 Liu et al.


Kang H.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Fan Y.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Sun A.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education | Deng X.,Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education
Experimental Biology and Medicine | Year: 2011

We hypothesized that diminished endothelial glycocalyx (GCX) at atherosclerotic lesion-prone sites accelerates flowdependent concentration polarization of low-density lipoproteins (LDLs) at the luminal surface, and in turn contributes to vulnerability of these sites to atherosclerosis. A parallel plate flow chamber was applied to expose cultured endothelial monolayers to three different levels of shear stress (3, 12, 20 dyn/cm2). Heparinase III (Hep.III) was employed to degrade heparan sulfate proteoglycans selectively and 3-(N-morpholino) propanesulfonic acid-buffered physiological salt solutions (MOPS-PSS) were used at either normal ionic strength (Normal-MOPS), low ionic strength (LO-MOPS) or high ionic strength (HI-MOPS) to modify the effective charge density of the endothelial GCX. Water filtration velocity (Vw) across the endothelial monolayer, the luminal concentration of LDLs (C w) and the uptake of LDLs by endothelial cells were measured and compared among the following five groups of cells: (1) Control; (2) Hep.III treatment; (3) LO-MOPS; (4) Normal-MOPS; and (5) HI-MOPS. The results obtained substantiated the aforementioned hypothesis and demonstrated that compositional or charge density modification of the endothelial GCX facilitated water filtration across the endothelium, enhanced the accumulation of LDLs on the luminal surface and increased the uptake of LDLs by endothelial cells, therefore contributing to atherogenesis. © 2011 by the Society for Experimental Biology and Medicine.

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