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Yang Y.Y.,Shandong Academy of Agricultural Sciences | Yang Y.Y.,Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province | Yang Y.Y.,National Center for Vegetable Improvement Shandong Branch | Huo Y.M.,Shandong Academy of Agricultural Sciences | And 21 more authors.
Euphytica | Year: 2013

Cytoplasmic genetic male-sterility is used to produce hybrid onion (Allium cepa L.) seeds worldwide. In this paper, we present the results of research aimed toward identifying PCR-based markers linked to the Ms locus through amplified fragment length polymorphism (AFLP). After screening 512 AFLP primer combinations, only one AFLP fragment was identified as being flanking linked to the dominant Ms allele. Subsequently, the AFLP marker was converted into a sequence-characterized amplified region (SCAR) marker, designated as DNF-566, co-segregated with the dominant Ms allele in first backcross (BC1) segregated populations. Furthermore, we designed another molecular marker (RNS-357) co-segregated with the ms allele to identify different genotypes (i. e., MsMs, Msms, or msms). Both markers could be used for evaluating onion lines with different genetic backgrounds (including male-sterile lines, maintainer lines, male-fertile lines, and commercial based F1 hybrid cultivars). The results of this study indicate that maintainer plants could be directly selected by using these 2 SCAR markers in the onion breeding process, and this may contribute significantly toward breeding onion F1 hybrid cultivars. © 2012 Springer Science+Business Media Dordrecht.

Huo Y.,Shandong Academy of Agricultural Sciences | Huo Y.,Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province | Huo Y.,National Center for Vegetable Improvement Shandong Branch | Miao J.,Shandong Academy of Agricultural Sciences | And 20 more authors.
Plant Breeding | Year: 2012

In this study, we investigated differential gene expression in the flower buds of male-fertile and male-sterile pools by using the cDNA-sequence-related amplified polymorphism (SRAP) method and discovered a fragment that was specifically expressed in male-fertile pool. Cloning and sequence analysis revealed that this differentially expressed sequence corresponded to a pectin methylesterase gene in Allium cepa (AcPME). This sequence was deposited in GenBank (GU384209). In male-fertility-restored line, AcPME was specifically expressed in tiny buds but not in roots, leaves and bulbs. In male-sterile line and maintainer line, AcPME was not expressed in roots, leaves, bulbs or tiny buds. AcPME was differentially expressed during different flowering stages (I, II, III, IV and V). We developed a PCR-based marker (WHR240) associated with the expression of AcPME. This marker was validated in six other onion lines; in each case, the male-fertility-restored onion was reliably identified. WHR240 therefore permits the efficient marker-assisted selection of maintainer individuals in onion breeding programmes. © 2011 Blackwell Verlag GmbH.

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