Kerala Veterinary and Animal Sciences University
Kerala, India

Kerala Veterinary and Animal science University is a university established by the Government of Kerala in December 2010 to further education, research and extension services in the field of Veterinary and Animal science. The territorial jurisdiction of the university extends to the whole of the State of Kerala. Its headquarters is located at Pookode in Wayanad District in Kerala State. There are three constituent colleges to the university:College of Veterinary and Animal science at Mannuthy, College of Veterinary and Animal science at Pookot and College of Dairy Science and Technology at Mannuthy. Dr B Ashok, an alumnus of College of Veterinary and Animal science at Mannuthy, is the first Vice-Chancellor of the University.The foundation stone-laying ceremony of the administrative block of the university was held on 2 August 2010 by V.S. Achuthanandan, Chief Minister of Kerala. Wikipedia.

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Radhika G.,Kerala Veterinary and Animal Sciences University | Aravindakshan T.V.,Kerala Veterinary and Animal Sciences University | Jinty S.,Kerala Veterinary and Animal Sciences University | Ramya K.,Kerala Veterinary and Animal Sciences University
Animal Biotechnology | Year: 2017

The legendary Vechur cattle of Kerala, described as a very short breed, and the crossbred (CB) Sunandini cattle population exhibited great phenotypic variation; hence, the present study attempted to analyze the genetic diversity existing between them. A set of 14 polymorphic microsatellites were chosen from FAO-ISAG panel and amplified from genomic DNA isolated from blood samples of 30 Vechur and 64 unrelated crossbred cattle, using fluorescent labeled primers. Both populations revealed high genetic diversity as evidenced from high observed number of alleles, Polymorphic Information Content and expected heterozygosity. Observed heterozygosity was lesser (0.699) than expected (0.752) in Vechur population which was further supported by positive FIS value of 0.1149, indicating slight level of inbreeding in Vechur population. Overall, FST value was 0.065, which means genetic differentiation between crossbred and Vechur population was 6.5%, indicating that the crossbred cattle must have differentiated into a definite population that is different from the indigenous Vechur cows. Structure analysis indicated that the two populations showed distinct differences, with two underlying clusters. The present study supports the separation between Taurine and Zebu cattle and throws light onto the genetic diversity and relationship between native Vechur and crossbred cattle populations in Kerala state. © 2017 Taylor & Francis

Thomas M.,Kerala Veterinary and Animal Sciences University | Thomas J.,Kerala Veterinary and Animal Sciences University
Indian Veterinary Journal | Year: 2017

An investigation was conducted to analyse the comparative productive performance of brucella sero-positive and seo-negative cattle of an organized crossbred dairy herd. Overall lactation yield, 305-day milk production and lactation length were significantly (p≤0.05) lower among sero-positive cows compared to seo-negative cattle. These differences are more marked (p≤0.05) among first calvers where the lactation yield of sero positive animals was lower by 33. 01%. The average daily milk yield and peak yield were similar in both groups.

Stephen M.,Kerala Veterinary and Animal Sciences University
Indian Veterinary Journal | Year: 2016

Peak yield and 305-day milk yields of higher grade crossbreds with exotic inheritance more than 50 per cent reared in a farm in Kerala was studied. The leastsquares mean peak yield and 305-day milk yield were 16.4 ± 0.32 kg and 3437.6 ± 88.92 kg, respectively. Leastsquares analysis of variance showed that year of calving and parity significantly affected the peak yield and 305-day milk yield whereas the genetic group variation and seasons of calving considered in the model were not significant factors affecting both the traits. © 2016, Indian Veterinary Assocaition. All rights reserved.

Behera S.,Veterinary College | Harshan H.M.,Kerala Veterinary and Animal Sciences University | Lekshmi Bhai K.L,Kerala Veterinary and Animal Sciences University | Aravinda Ghosh K.N.,Kerala Veterinary and Animal Sciences University
Veterinary World | Year: 2015

Aim: Sperm membrane cholesterol influences cryodamage during cryopreservation. The present study was carried out to evaluate the effect of varying cholesterol levels in Tris based extenders on the freezability of sexually healthy Malabari buck semen. Materials and Methods: A total of 48 ejaculates from two adults healthy sexually healthy Malabari bucks were utilized for the study. The collected and pooled ejaculates were divided into four groups with Group I serving as Control - I, Group II and III were treated with 1 mg and 2 mg of cholesterol-loaded-cyclodextrin (CLC)/120 × 106 spermatozoa, respectively, and Group IV treated with 1 mg methyl-β-cyclodextrin (MβCD) served as Control - II. Manual freezing was carried out to cryopreserve the treated and control spermatozoa. Results: Treatment of semen samples with CLC resulted in improved maintenance of sperm motility at pre-freeze and post-thaw stages of cryopreservation without affecting hypo-osmotic swelling response. Treatment of semen with 1 mg of CLC/120 × 106 spermatozoa was observed to be better than treatment with 2 mg of CLC/120 × 106 spermatozoa. In general, MβCD treatment was found to result in significantly lower sperm characteristics than those of Control - I and CLC treatment at pre-feeze and post-thaw stages and when incubated up to 4 h. Conclusion: Cholesterol treatment of sexually healthy Malabari buck semen was found to hold promise for improving cryopreservability of spermatozoa. © The authors.

Sunilkumar S.,Kerala Veterinary and Animal Sciences University | Radhakrishnan U.,Kerala Veterinary and Animal Sciences University | Pala S.,Kerala Veterinary and Animal Sciences University
Indian Journal of Animal Sciences | Year: 2017

In the present study, a loop-mediated isothermal amplification assay targeting the ompA gene of R. anatipestifer was standardised using three freeze dried isolates of the organism. The reaction could be completed in a short time i.e. approximately an hour, as compared to PCR which requires about 3 h for completion. The whole isothermal reaction was performed in a simple water bath and the positive reaction could be detected with naked eye, thereby confirming that no expensive equipment is needed for the diagnosis of the pathogen effectively. This technique could be mastered by veterinarians and laboratory technicians very easily compared to PCR; thereby reducing the time lag in reaching a confirmative diagnosis. This assay could be carried out in a local laboratory without any special equipment and high edge facilities.Once properly standardized, the LAMP assay serves as a very simple, rapid and effective pen-side diagnostic technique. It has the potential to revolutionize the field of human and veterinary disease diagnosis, thereby aiding in adopting prompt control and therapeutic strategies.

Chandran A.,Kerala Veterinary and Animal Sciences University | Grover S.,National Dairy Research Institute | Batish V.K.,National Dairy Research Institute
Journal of Tropical Agriculture | Year: 2014

Lactobacillus plantarum is a flexible and versatile microorganism that inhabits a wide variety of environmental niches, including the human gastrointestinal (GI) tract. Strains of Lactobacillus plantarum have proven ability to survive gastric transit and can colonize the intestinal tract of humans and other mammals. The ability of these microorganisms to grow in harsh environment prevailing in is linked to their ability to resist acidic conditions in the stomach of healthy humans. Changes in pH in the environment have been reported to influence the expression of many genes and atp operon is chiefly involved in the acid tolerance of probiotic lactobacilli in the gut. The atp genes are included in the category of housekeeping genes. However, the regulation of this pH-inducible phenotype has not been clearly established at the molecular level. In this study the influence of low pH on inducible gene expression in Lactobacillus plantarum 91 was investigated both in vitro and in vivo. Logarithmic phase cultures were exposed to pH 2.5, 2.0 and 1.5 for various time intervals and cultured for monitoring survivability. The cultures were able to survive at pH 1.5 to an appreciable level even after 1-3 h. In vivo study was carried out by feeding L.plantarum cultures to mice followed by isolation of bacterial RNA from stomach at different time intervals. The isolated RNA was reverse transcribed and the resultant cDNAs were subjected to RT-qPCR and the products were resolved by electrophoresis. The atpD gene was significantly up-regulated to 1.48, 2.04 and 3.05 folds after 15, 30 and 60 min. transit in the stomach of mice. This result clearly demonstrates that atpD gene expression is essential for survival of probiotic bacteria under acidic environment prevailing in the stomach. © 2014, Kerala Agricultural University. All rights reserved.

Panicker V.P.,Kerala Veterinary and Animal Sciences University | George S.,Kerala Veterinary and Animal Sciences University
International Journal of Peptide Research and Therapeutics | Year: 2016

A putative cathelicidin antimicrobial peptide of 34 amino acid residues was deduced from buffalo myeloid gene sequences and named as Buffalo myeloid antimicrobial peptide-34 (BuMAP-34). Structure–function relationship of the custom synthesized peptide was evaluated in vitro. Highly cationic, amphipathic peptide showed a net charge of +6 and predicted hydrophobic ratio of 38 %. Phylogenetic analysis revealed an evolutionary relationship with Bovine myeloid antimicrobial peptide-34 (BMAP-34) of cattle, myeloid antimicrobial peptide-34 (MAP-34) of Goat and Sheep myeloid antimicrobial peptide-34 (SMAP-34). Peptide showed potent antimicrobial activity against a wide spectrum of microorganisms including Gram-negative and Gram-positive bacteria and fungi. Minimum inhibitory concentration (MIC) on various strains of bacteria, and fungus ranged from 1.1 to 1.5 µM except for P. multocida multocida (HS), which was >100 µM. Scanning electron microscopic (SEM) analysis of the peptide treated E. coli, S. aureus and C. albicans indicated cell lysis. Peptide also showed its ability to bind with anionic components of the cells which was confirmed by DNA binding assay. Haemolytic activity assay revealed absence of haemolysis in human RBCs at 12.5 µM and in sheep RBCs even at 100 µM concentration of the peptide. The present study suggests that the cathelicidin, BuMAP-34 has strong antimicrobial activity and could be developed as a promising broad spectrum antimicrobial agent. © 2016 Springer Science+Business Media New York

Panicker V.P.,Kerala Veterinary and Animal Sciences University | George S.,Kerala Veterinary and Animal Sciences University
Indian Journal of Biochemistry and Biophysics | Year: 2013

Antimicrobial peptides (AMPs) are broad spectrum antibiotics, which mostly act without specific receptors. Identification of AMPs is important in the current scenario of emerging multi-drug resistant bacteria. In the present study, in an attempt to identify new AMPs, myeloid cathelicidin cDNAs were synthesized from buffalo (Bubalus bubalis) bone marrow and were amplified using specific primers. Sequence analysis of cloned cDNAs revealed three novel myeloid cathelicidins. They were named based on the number of active amino acids in the C-terminal region of their predicted peptide sequences as BuMAP-28 (having an additional Gly at position 22nd), BuMAP-29 (having an additional IIe at position 27) and BuMAP-34, compared to BMAP-27, BMAP-28 and BMAP-34 of cattle. The BuMAPs showed 93%, 95% and 87% homology respectively with that of its cattle counterpart. Predicted number of amino acids of the cDNAs was 159, 155 and 157 residues, with cationic C-terminal sequences of 28, 29 and 34, respectively, which correspond to putative antimicrobial domains. Several amino acid substitutions were observed in all the three cathelicidins. The conformation of the peptides was predicted to be alpha helical, having total net positive charge and hydrophobicity, similar to that of BMAPs in cattle. Comparative analysis of the predicted peptides suggested potential antimicrobial activity and the sequence variations detected might enable the peptides to act as effective broad spectrum antimicrobial agents.

PubMed | Indian Veterinary Research Institute and Kerala Veterinary and Animal Sciences University
Type: | Journal: Research in veterinary science | Year: 2017

Caprine amniotic fluid (cAF) and bone marrow cells (cBM) were isolated, expanded and phenotypically characterized by mesenchymal stem cells (MSCs) specific cell surface markers. Both cell types were compared for multilineage differentiation potential by flow cytometry using specific antibodies against lineage specific markers. Furthermore, in vitro expanded cAF-MSCs showed higher expression of trophic factors viz. VEGF and TGF-1 as compared to cBM-MSCs. Full-skin thickness excisional wounds created on either side of the dorsal midline (thoracolumbar) of New Zealand White rabbits were randomly assigned to subcutaneous injection of either fetal origin cAF-MSCs (n=4) or adult cBM-MSCs (n=4) or sterile PBS (control, n=4). The rate of wound closure was found faster (p<0.05) in cAF-MSCs treated wounds as compared with cBM-MSCs and PBS treated wounds especially on 21st day post-skin excision. Histomorphological examination of the healing tissue showed that wound healing was improved (p<0.05) by greater epithelialization, neovascularization and collagen development in cAF-MSCs as compared to cBM-MSCs and PBS treated wounds.

Pruthviraj D.R.,Kerala Veterinary and Animal Sciences University | Usha A.P.,Kerala Veterinary and Animal Sciences University | Venkatachalapathy R.T.,Kerala Veterinary and Animal Sciences University
Asian-Australasian Journal of Animal Sciences | Year: 2016

Porcine beta-defensin-1 (PBD-1) gene plays an important role in the innate immunity of pigs. The peptide encoded by this gene is an antimicrobial peptide that has direct activity against a wide range of microbes. This peptide is involved in the co-creation of an antimicrobial barrier in the oral cavity of pigs. The objective of the present study was to detect polymorphisms, if any, in exon-1 and exon-2 regions of PBD-1 gene in Large White Yorkshire (LWY) and native Ankamali pigs of Kerala, India. Blood samples were collected from 100 pigs and genomic DNA was isolated using phenol chloroform method. The quantity of DNA was assessed in a spectrophotometer and quality by gel electrophoresis. Exon-1 and exon-2 regions of PBD-1 gene were amplified by polymerase chain reaction (PCR) and the products were subjected to single strand conformation polymorphism (SSCP) analysis. Subsequent silver staining of the polyacrylamide gels revealed three unique SSCP banding patterns in each of the two exons. The presence of single nucleotide polymorphisms (SNPs) was confirmed by nucleotide sequencing of the PCR products. A novel SNP was found in the 5'-UTR region of exon-1 and a SNP was detected in the mature peptide coding region of exon-2. In exon-1, the pooled population frequencies of GG, GT, and TT genotypes were 0.67, 0.30, and 0.03, respectively. GG genotype was predominant in both the breeds whereas TT genotype was not detected in LWY breed. Similarly, in exon-2, the pooled population frequencies of AA, AG, and GG genotypes were 0.50, 0.27, and 0.23, respectively. AA genotype was predominant in LWY pigs whereas GG genotype was predominant in native pigs. These results suggest that there exists a considerable genetic variation at PBD-1 locus and further association studies may help in development of a PCR based genotyping test to select pigs with better immunity. Copyright © 2016 by Asian-Australasian Journal of Animal Sciences.

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