Manju M.,Kerala University |
Manju M.,P.A. College |
Vijayasree A.S.,Kerala University |
Akbarsha M.A.,Bharathidasan University |
Oommen O.V.,Kerala State Biodiversity Board
Fish Physiology and Biochemistry | Year: 2013
The present study was conducted to evaluate the safety of long-term dietary curcumin at doses 0.5 and 1 % in Anabas testudineus employing hematological and cytological techniques. The fish were fed with curcumin-supplemented feed for 6 months. Fine blood smears were prepared and subjected to three different staining techniques. The erythrocyte micronucleus frequency (MN) and the cytometric measurements of erythrocytes were determined. Blood from the control and treated fish was subjected to the assessment of several hematological parameters. Also, DNA fragmentation assay on hepatocytes was conducted. The results showed that hemoglobin content, RBC count and hematocrit increased in the curcumin-fed fish compared to control, whereas WBC count, platelet count, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were unaffected. WBC/RBC ratio was lower in the case of curcumin-treated fish. The cytometric measurements revealed no change in the erythrocytes and their nuclei after curcumin treatment. DNA fragmentation assay revealed intact DNA in curcumin-fed group, ruling out the possibility of curcumin-induced DNA damage. The positive control group showed a significant increase in MN frequency compared to negative control and curcumin-fed groups. In fact, the MN frequency decreased in 1 % curcumin-fed group compared to the negative control and 0.5 % curcumin groups. All these indicated a state of well-being of the curcumin-treated fish. Therefore, it is concluded that curcumin could be used as a safe feed ingredient to improve the growth of finfish in aquaculture. © 2013 Springer Science+Business Media Dordrecht.
Midhun S.J.,Central University of Kerala |
Arun D.,Central University of Kerala |
Edatt L.,Central University of Kerala |
Sruthi M.V.,Central University of Kerala |
And 5 more authors.
Aquaculture International | Year: 2016
Aquaculture is faced with the challenges of the use of synthetic compounds as growth enhancers and the presence of several contaminants in water. These factors severely deteriorate the quality and quantity of aquaculture products. Phytochemicals play a major role by working as antioxidant agents of which curcumin has become the gold standard. Curcumin, from Curcuma longa shows a wide spectrum of biological activities which include anticancerous, antioxidant, anti-inflammatory, antibacterial, antiviral, antifungal, antidiabetic, antistress, hepatoprotective and gastroprotective effects. Curcumin in 0.5 and 1 % doses were given as feed additive to Oreochromis mossambicus for 35 days. After feeding trial, activities of digestive enzymes such as α-amylase, protease and lipase were analysed. There was a significant increase in the activities of α-amylase, protease and lipase with 0.5 and 1 % curcumin supplementation in feed. Real-time quantification of GH in brain, and IGF-1 and IGF-2 genes in muscle revealed that curcumin significantly increased the expression of these genes. This is the first study to report that curcumin supplementation at concentrations of 0.5 and 1 % in the feed improved the activities of digestive enzymes and also modulates the expression of GH in brain and growth factors such as IGF-1 and IGF-2 in muscle of O. mossambicus. © 2016 Springer International Publishing Switzerland
Kotharambath R.,Government College Chittur |
Wilkinson M.,Natural History Museum in London |
Oommen O.V.,Kerala State Biodiversity Board |
Gower D.J.,Natural History Museum in London
Zootaxa | Year: 2015
A new species of indotyphlid caecilian amphibian, Gegeneophis tejaswini sp. nov., is described based on eight specimens from lowlands of the most northerly district of the state of Kerala in the southern part of the Western Ghats region, India. This species is distinguished from all other Gegeneophis in annulation characters and genetics (> 6% different from most similar nominal species for 883 base pairs of mitochondrial 12S and 16S rRNA gene sequence data). The high degree of morphological similarity of G. krishni, G. mhadeiensis and the new species underlines that, for some Gegeneophis, larger samples and/or new characters will be needed to further advance the taxonomy of this genus. Copyright © 2015 Magnolia Press.
Baijulal B.,Kerala State Biodiversity Board |
Sobha V.,Kerala University |
Jyothi S.J.,Kerala University |
Padmalal D.,Center for Earth Science Studies |
Emmanuel J.,St Johns College Anchal
Environmental Monitoring and Assessment | Year: 2013
The present paper deals with the distribution of sediment and sediment-bound nutrients in two important coastal lagoons of southern Kerala such as the Ashtamudi Estuarine Lagoon in the Kollam district and the Kadinamkulam Lagoon in the Thiruvananthapuram district. Among the two lagoons, the former is coast perpendicular, and the latter is coast parallel. An analysis of the textural characteristics reveals that, in both lagoons, the estuarine mouth and areas close to it are dominated by sand and sand-rich sediment species, indicating a high-energy depositional regime prevailing the region. On the other hand, the silt and clay dominant arms are almost sheltered and enjoy a low-energy depositional environment. The nutrient and organic carbon contents in the sheltered areas are significantly higher than the most dynamic high-energy estuarine mouth regions. This peculiar behaviour of these coastal water bodies has to be given adequate importance while laying down strategies for the conservation and management of these fragile aquatic systems in the south-western coast of India in particular and tropical coasts of the world in general. © 2013 Springer Science+Business Media Dordrecht.
Shaji C.P.,Kerala State Biodiversity Board |
Laladhas K.P.,Kerala State Biodiversity Board
Indian Journal of Traditional Knowledge | Year: 2013
The breeding migration of the freshwater fishes is known locally as Oothayilakkom or Ootha. A study has been conducted during 2010 to study the fishery and fishing gears during Ootha. The traditional fishing gears, its fabrication and operation in Thrissur district were studied. Adichil, Chaattom, Nathoodu are gears unique to the study area. The impacts Ootha fishery on the sustainability of the fish resources and eco-friendliness of these gears were evaluated.
Das P.,Kerala University |
Dileepkumar R.,Kerala University |
Krishnan A.,Kerala University |
Nair A.S.,Kerala University |
Oommen O.V.,Kerala State Biodiversity Board
Journal of Entomological Research | Year: 2013
Antibacterial activity of gastric secretions from an ant, Oecophylla smaragdina was evaluated against three gram positive bacteria: Staphylococcus aureus, Bacillus subtilis, Staphylococcus epidermidis and four gram negative bacteria: Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Vibrio cholerae. Excellent antibacterial activity was exhibited against Vibrio cholerae, Bacillus subtilis and Staphylococcus epidermidis. Moderate effects were seen for other pathogens being tested.
PubMed | Kerala State Biodiversity Board
Type: Journal Article | Journal: Environmental monitoring and assessment | Year: 2013
The present paper deals with the distribution of sediment and sediment-bound nutrients in two important coastal lagoons of southern Kerala such as the Ashtamudi Estuarine Lagoon in the Kollam district and the Kadinamkulam Lagoon in the Thiruvananthapuram district. Among the two lagoons, the former is coast perpendicular, and the latter is coast parallel. An analysis of the textural characteristics reveals that, in both lagoons, the estuarine mouth and areas close to it are dominated by sand and sand-rich sediment species, indicating a high-energy depositional regime prevailing the region. On the other hand, the silt and clay dominant arms are almost sheltered and enjoy a low-energy depositional environment. The nutrient and organic carbon contents in the sheltered areas are significantly higher than the most dynamic high-energy estuarine mouth regions. This peculiar behaviour of these coastal water bodies has to be given adequate importance while laying down strategies for the conservation and management of these fragile aquatic systems in the south-western coast of India in particular and tropical coasts of the world in general.
PubMed | Kerala University and Kerala State Biodiversity Board
Type: Journal Article | Journal: Journal of ethnopharmacology | Year: 2013
The folklore or traditional therapy in southern India widely utilizes a plethora of local herbs to treat the patients challenged with snake venom. Despite the widespread implementation of antisera therapy, the local population of the country still relies on this centurys old medicinal formulas mainly due to the cost effectiveness, lesser side effects and also its cultural acceptability. The present study aims to validate the neutralizing ability of one such traditionally acclaimed antidote Ophiorrhiza mungos root extract against Russells viper (Daboia russelii) venom in the early developing chick embryos.The disc impregnated with venom, root extract or the combination of both was placed on the yolk sac membrane preferably over the anterior blood vessel of 6th day chick embryo. The neutralization/inhibition of venom-induced lethality or hemorrhage was achieved by incubating venom and extract before being applied to the embryo. The membrane stabilizing properties of root extract was estimated by HRBC lysis method. The preliminary phytochemical analysis was done to assess the phyto constituents in the root extract.The LD50 of Russells viper venom in 6th day chick embryo was found to be 3 g/l. The neutralising effect of root extract was achieved by pre-incubating venom with various concentrations of extract and at the concentration of 10 g/l, 100% recovery of embryos was observed after 6h of incubation. Higher concentration of root extract showed remarkable results by completely abolishing traces of hemorrhagic lesions induced by viper venom.The above observations confirmed that the root extract of Ophiorrhiza mungos possess potent anti snake venom neutralizing compounds, which inhibit the activity of viper venom. The chick embryo, a new insensate model used in the present study is significant in venom research as it reduces the ruthless suffering of higher mammalian experimental models.