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Yabe S.,Kennan Eisei Kogyo Co. | Aiba Y.,Kennan Eisei Kogyo Co. | Sakai Y.,Kennan Eisei Kogyo Co. | Hazaka M.,Kennan Eisei Kogyo Co. | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A Gram-negative bacterium, designated CKTN2T, was isolated from compost. Cells of strain CKTN2T were strictly aerobic rods. The isolate grew at 20-50 °C (optimum 40-45 °C), but not below 15 °C or above 52 °C, and at pH 5.9-8.8 (optimum pH 7.0), but not below pH 5.4 or above pH 9.3. The DNA G+C content was 40.3 mol%. The predominant menaquinone was MK- 7. The major fatty acids were iso-C15:0 (45.2 %), iso-C17:0 3-OH (11.1 %) and C18: 0 (14.5 %). Analysis of the 16S rRNA gene sequence of strain CKTN2T revealed that it is a member of the genus Sphingobacterium and is most closely related to Sphingobacterium alimentarium DSM 22362T (93.2% 16S rRNA gene sequence similarity). Strain CKTN2T could be distinguished from its closest phylogenetic relatives by different phenotypic characteristics. According to the phenotypic and genotypic characteristics, strain CKTN2T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium thermophilum sp. nov. is proposed. The type strain is CKTN2T (5JCM 17858T 5KCTC 23708T). © 2013 IUMS. Source


Yabe S.,Tohoku University | Yabe S.,Kennan Eisei Kogyo Co. | Sakai Y.,Tohoku University | Sakai Y.,Kennan Eisei Kogyo Co. | Yokota A.,Tohoku University
International Journal of Systematic and Evolutionary Microbiology | Year: 2016

A thermophilic, Gram-stain-positive, spore-forming bacterium that formed branched vegetative and aerial mycelia was isolated from fallen leaves on geothermal soil. This strain, designated F4T, grew at temperatures between 30 and 60°C; optimum growth temperature was 50°C, whereas no growth was observed below 28°C or above 65°C. The pH range for growth was 4.9–9.5; the pH for optimum growth was 7.0, but no growth was observed at pH below 4.4 or above 10.0. Strain F4T was able to hydrolyse polysaccharides such as cellulose, xylan, chitin and starch. The G+C content in the DNA of strain F4T was 52.5 mol%. The major fatty acid was iso-C17: 0 and the major menaquinone was MK-9 (H2). The cell wall of strain F4T contained glutamic acid, serine, glycine, alanine and ornithine in a molar ratio of 1.0:1.5:1.4:1.8:0.7. The polar lipids of this strain consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unknown phospholipid, three unknown glycolipids and two unknown lipids. The cell-wall sugar was mannose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain F4T belongs to the genus Thermosporothrix, and that it was related most closely to Thermosporothrix hazakensis SK20-1T (98.7 % similarity). DNA–DNA hybridization showed relatedness values of less than 15 % with the type strain of Thermosporothrix hazakensis. On the basis of phenotypic features and phylogenetic position, strain F4T is considered to represent a novel species, Thermosporothrix narukonensis sp. nov. The type strain is F4T(=NBRC 111777T=BCCM/LMG 29329T). © 2016 IUMS. Source


Park J.-S.,University of Tokyo | Kagaya N.,Technology Research Association for Next Generation Natural Product Chemistry | Hashimoto J.,Japan Biological Informatics Consortium JBiC | Izumikawa M.,Japan Biological Informatics Consortium JBiC | And 4 more authors.
ChemBioChem | Year: 2014

Two new acyloin compounds were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20-1T. Genome sequencing of the bacterium and biochemical studies identified the thiamine diphosphate (TPP)-dependent enzyme Thzk0150, which is involved in the formation of acyloin. Through extensive analysis of the Thzk0150-catalyzed reaction products, we propose a putative reaction mechanism involving two substrates: 4-methyl-2-oxovalerate as an acyl donor and phenyl pyruvate as an acyl acceptor. Discovery from a thermophile: Two new acyloins with R configurations at their chiral centers were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20-1T. These molecules were synthesized from two keto acid substrates by TPP-dependent Thzk0150 enzyme-catalyzed Cï£ ¿C bond condensation. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Patent
Kennan Eisei Kogyo Co. | Date: 2012-05-31

This invention provides a novel cellulase derived from


Yabe S.,Kennan Eisei Kogyo Co. | Aiba Y.,University of Tokyo | Sakai Y.,Kennan Eisei Kogyo Co. | Hazaka M.,Kennan Eisei Kogyo Co. | Yokota A.,University of Tokyo
International Journal of Systematic and Evolutionary Microbiology | Year: 2011

Two thermophilic, Gram-stain-positive, sporulating bacterial strains, which formed branched vegetative and aerial mycelia, were isolated from fallen leaves sampled from geothermal soils and designated ONI-1T and ONI-5T. Strain ONI-1T grew at 50-74 °C, with optimum growth at 60-65 °C, and strain ONI-5T grew at 45-74 °C, with optimum growth at 60-65 °C. The pH range for growth of the strains was pH 4.6-8.0, with optimum growth at pH 7.0. The DNA G+C contents of strains ONI-1T and ONI-5T were 60.2 and 58.1 mol%, respectively. The major fatty acid was iso-C17: 0 and the major menaquinone was MK-9(H2). The cell walls of the strains contained glutamic acid, serine, glycine, histidine, alanine and ornithine. The polar lipids consisted of phosphatidylinositol, phosphatidylglycerol and a glycolipid. The cell-wall sugar was rhamnose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belong to the class Ktedonobacteria and that strains ONI-1T and ONI-5T are most closely related to Thermosporothrix hazakensis SK20-1T (85.3 and 84.5% sequence similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.6%. Based on the phenotypic features and phylogenetic position, we propose that strains ONI-1T and ONI-5T constitute a novel genus containing two novel species, for which we propose the names Thermogemmatispora onikobensis gen. nov., sp. nov. (the type species; type strain ONI-1T =JCM 16817T =KCTC 19768T) and Thermogemmatispora foliorum sp. nov. (type strain ONI-5T =JCM 16818T =KCTC 19767T), within the new family Thermogemmatisporaceae fam. nov. and order Thermogemmatisporales ord. nov. © 2011 IUMS. Source

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