Kennan Eisei Kogyo Co.

Shibata, Japan

Kennan Eisei Kogyo Co.

Shibata, Japan

Time filter

Source Type

Yabe S.,Tohoku University | Yabe S.,Kennan Eisei Kogyo Co. | Sakai Y.,Tohoku University | Sakai Y.,Kennan Eisei Kogyo Co. | And 2 more authors.
Microbes and Environments | Year: 2017

Bacteria with an actinomycetes-like morphology have recently been discovered, and the class Ktedonobacteria was created for these bacteria in the phylum Chloroflexi. They may prove to be a valuable resource with the potential to produce unprecedented secondary metabolites. However, our understanding of their diversity, richness, habitat, and ecological significance is very limited. We herein developed a 16S rRNA gene-targeted, Ktedonobacteria-specific primer and analyzed ktedonobacterial amplicons. We investigated abundance, diversity, and community structure in forest and garden soils, sand, bark, geothermal sediment, and compost. Forest soils had the highest diversity among the samples tested (1181–2934 operational taxonomic units [OTUs]; Chao 1 estimate, 2503–5613; Shannon index, 4.21–6.42). A phylogenetic analysis of representative OTUs revealed at least eight groups within unclassified Ktedonobacterales, expanding the known diversity of this order. Ktedonobacterial communities markedly varied among our samples. The common mesic environments (soil, sand, and bark) were dominated by diverse phylotypes within the eight groups. In contrast, compost and geothermal sediment samples were dominated by known ktedonobacterial families (Thermosporotrichaceae and Thermogemmatisporaceae, respectively). The relative abundance of Ktedonobacteria in the communities, based on universal primers, was ≤0.8%, but was 12.9% in the geothermal sediment. These results suggest that unknown diverse Ktedonobacteria inhabit common environments including forests, gardens, and sand at low abundances, as well as extreme environments such as geothermal areas. © 2017, Japanese Society of Microbial Ecology. All rights reserved.


Yabe S.,Tohoku University | Yabe S.,Kennan Eisei Kogyo Co. | Sakai Y.,Tohoku University | Sakai Y.,Kennan Eisei Kogyo Co. | Yokota A.,Tohoku University
International Journal of Systematic and Evolutionary Microbiology | Year: 2016

A thermophilic, Gram-stain-positive, spore-forming bacterium that formed branched vegetative and aerial mycelia was isolated from fallen leaves on geothermal soil. This strain, designated F4T, grew at temperatures between 30 and 60°C; optimum growth temperature was 50°C, whereas no growth was observed below 28°C or above 65°C. The pH range for growth was 4.9–9.5; the pH for optimum growth was 7.0, but no growth was observed at pH below 4.4 or above 10.0. Strain F4T was able to hydrolyse polysaccharides such as cellulose, xylan, chitin and starch. The G+C content in the DNA of strain F4T was 52.5 mol%. The major fatty acid was iso-C17: 0 and the major menaquinone was MK-9 (H2). The cell wall of strain F4T contained glutamic acid, serine, glycine, alanine and ornithine in a molar ratio of 1.0:1.5:1.4:1.8:0.7. The polar lipids of this strain consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unknown phospholipid, three unknown glycolipids and two unknown lipids. The cell-wall sugar was mannose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain F4T belongs to the genus Thermosporothrix, and that it was related most closely to Thermosporothrix hazakensis SK20-1T (98.7 % similarity). DNA–DNA hybridization showed relatedness values of less than 15 % with the type strain of Thermosporothrix hazakensis. On the basis of phenotypic features and phylogenetic position, strain F4T is considered to represent a novel species, Thermosporothrix narukonensis sp. nov. The type strain is F4T(=NBRC 111777T=BCCM/LMG 29329T). © 2016 IUMS.


Yabe S.,Kennan Eisei Kogyo Co. | Aiba Y.,University of Tokyo | Sakai Y.,Kennan Eisei Kogyo Co. | Hazaka M.,Kennan Eisei Kogyo Co. | Yokota A.,University of Tokyo
International Journal of Systematic and Evolutionary Microbiology | Year: 2011

Two thermophilic, Gram-stain-positive, sporulating bacterial strains, which formed branched vegetative and aerial mycelia, were isolated from fallen leaves sampled from geothermal soils and designated ONI-1T and ONI-5T. Strain ONI-1T grew at 50-74 °C, with optimum growth at 60-65 °C, and strain ONI-5T grew at 45-74 °C, with optimum growth at 60-65 °C. The pH range for growth of the strains was pH 4.6-8.0, with optimum growth at pH 7.0. The DNA G+C contents of strains ONI-1T and ONI-5T were 60.2 and 58.1 mol%, respectively. The major fatty acid was iso-C17: 0 and the major menaquinone was MK-9(H2). The cell walls of the strains contained glutamic acid, serine, glycine, histidine, alanine and ornithine. The polar lipids consisted of phosphatidylinositol, phosphatidylglycerol and a glycolipid. The cell-wall sugar was rhamnose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belong to the class Ktedonobacteria and that strains ONI-1T and ONI-5T are most closely related to Thermosporothrix hazakensis SK20-1T (85.3 and 84.5% sequence similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.6%. Based on the phenotypic features and phylogenetic position, we propose that strains ONI-1T and ONI-5T constitute a novel genus containing two novel species, for which we propose the names Thermogemmatispora onikobensis gen. nov., sp. nov. (the type species; type strain ONI-1T =JCM 16817T =KCTC 19768T) and Thermogemmatispora foliorum sp. nov. (type strain ONI-5T =JCM 16818T =KCTC 19767T), within the new family Thermogemmatisporaceae fam. nov. and order Thermogemmatisporales ord. nov. © 2011 IUMS.


Yabe S.,Kennan Eisei Kogyo Co. | Aiba Y.,University of Tokyo | Sakai Y.,Kennan Eisei Kogyo Co. | Hazaka M.,Kennan Eisei Kogyo Co. | Yokota A.,University of Tokyo
International Journal of Systematic and Evolutionary Microbiology | Year: 2011

A thermophilic, Gram-positive bacterium that formed a branched vegetative mycelium was isolated from compost. The strain, designated I3T, grew at temperatures between 35 and 62 °C, with optimum growth at 50-55 °C. No growth was observed below 29 °C or above 65 °C. The pH range for growth was 5.7-10.0, the pH for optimum growth was 7.0 and no growth was observed below pH 5.6 or above pH 10.8. The DNA G+C content of strain I3 T was 69.2 mol%. The major fatty acids found were C15 : 0 iso (14.2 %), C15 : 0 anteiso (12.1 %), C17 : 0 iso (16.3 %) and C17 : 0 anteiso (21.7 %). The major menaquinones were MK-9(H4), MK-10(H4) and MK-11(H4). The cell wall contained glutamic acid, glycine, alanine and LL-diaminopimelic acid in a molar ratio of 1.0 : 3.9 : 0.6 : 0.5. The polar lipids consisted of ninhydrin-positive phosphoglycolipids, phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid. The cell-wall sugars were rhamnose and arabinose. 16S rRNA gene sequence analysis assigned this actinomycete to the family Nocardioidaceae, but its 16S rRNA gene sequence shared no more than 95.5% similarity with those of other members of the family. The chemotaxonomic and phenotypic characteristics of strain I3T differed in some respects from those of members of the genus Actinopolymorpha, the most closely related genus. Therefore, strain I3T represents a novel species in a new genus of the family Nocardioidaceae, for which the name Thermasporomyces composti gen. nov., sp. nov. is proposed. The type strain of the type species is I3T (=JCM 16421T=DSM 22891 T). © 2011 IUMS.


Yabe S.,Kennan Eisei Kogyo Co. | Aiba Y.,Kennan Eisei Kogyo Co. | Sakai Y.,Kennan Eisei Kogyo Co. | Hazaka M.,Kennan Eisei Kogyo Co. | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A Gram-negative bacterium, designated CKTN2T, was isolated from compost. Cells of strain CKTN2T were strictly aerobic rods. The isolate grew at 20-50 °C (optimum 40-45 °C), but not below 15 °C or above 52 °C, and at pH 5.9-8.8 (optimum pH 7.0), but not below pH 5.4 or above pH 9.3. The DNA G+C content was 40.3 mol%. The predominant menaquinone was MK- 7. The major fatty acids were iso-C15:0 (45.2 %), iso-C17:0 3-OH (11.1 %) and C18: 0 (14.5 %). Analysis of the 16S rRNA gene sequence of strain CKTN2T revealed that it is a member of the genus Sphingobacterium and is most closely related to Sphingobacterium alimentarium DSM 22362T (93.2% 16S rRNA gene sequence similarity). Strain CKTN2T could be distinguished from its closest phylogenetic relatives by different phenotypic characteristics. According to the phenotypic and genotypic characteristics, strain CKTN2T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium thermophilum sp. nov. is proposed. The type strain is CKTN2T (5JCM 17858T 5KCTC 23708T). © 2013 IUMS.


Yabe S.,Kennan Eisei Kogyo Co. | Aiba Y.,University of Tokyo | Sakai Y.,Kennan Eisei Kogyo Co. | Hazaka M.,Kennan Eisei Kogyo Co. | Yokota A.,University of Tokyo
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

A Gram-stain-positive thermophilic bacterium, designated strain Nis3T, was isolated from compost. The strain grew at 23-57 °C (optimum, 50 °C); no growth was observed below 15 or above 60 °C. The pH range for growth was 5.9-8.8 (optimum, 7.0); no growth was observed below pH 5.4 or above pH 9.3. The DNA G+C content of strain Nis3T was 63.4 mol%. The dominant quinone type was ubiquinone Q-10. The major fatty acids were C18:1ω7c, C19:0ω8c cyclo and C18:0. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol, phosphatidylmonomethylethanolamine, an unknown glycolipid and a ninhydrin-positive phospholipid. 16S rRNA gene sequence analysis assigned this bacterium to the family Phyllobacteriaceae in the Alphaproteobacteria but it shared less than 95.2 % sequence similarity with other members of the family. The chemotaxonomic and phenotypic characteristics of strain Nis3T differed in some respects from those of members of the family Phyllobacteriaceae. Therefore, strain Nis3T is considered to represent a novel species of a new genus in the family Phyllobacteriaceae, for which the name Thermovum composti gen. nov., sp. nov. is proposed. The type strain is Nis3T (= JCM 17863T = KCTC 23707T). © 2012 IUMS.


Park J.-S.,University of Tokyo | Kagaya N.,Technology Research Association for Next Generation Natural Product Chemistry | Hashimoto J.,Japan Biological Informatics Consortium JBIC | Izumikawa M.,Japan Biological Informatics Consortium JBIC | And 4 more authors.
ChemBioChem | Year: 2014

Two new acyloin compounds were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20-1T. Genome sequencing of the bacterium and biochemical studies identified the thiamine diphosphate (TPP)-dependent enzyme Thzk0150, which is involved in the formation of acyloin. Through extensive analysis of the Thzk0150-catalyzed reaction products, we propose a putative reaction mechanism involving two substrates: 4-methyl-2-oxovalerate as an acyl donor and phenyl pyruvate as an acyl acceptor. Discovery from a thermophile: Two new acyloins with R configurations at their chiral centers were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20-1T. These molecules were synthesized from two keto acid substrates by TPP-dependent Thzk0150 enzyme-catalyzed Cï£ ¿C bond condensation. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Yabe S.,Kennan Eisei Kogyo Co. | Aiba Y.,University of Tokyo | Sakai Y.,Kennan Eisei Kogyo Co. | Hazaka M.,Kennan Eisei Kogyo Co. | Yokota A.,University of Tokyo
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

We isolated from compost an aerobic, thermophilic, Gram-stain-positive, spore-forming bacterium that formed branched vegetative and aerial mycelia. This strain, designated SK20-1T, grew at 31-58 °C, with optimum growth at 50 °C, while no growth was observed below 28 or above 60 °C. The pH range for growth was 5.4-8.7, with optimum growth at pH 7.0, while no growth was observed below pH 5.0 or above pH 9.1. Strain SK20-1T was able to hydrolyse polysaccharides such as cellulose, xylan and chitin. The DNA G+C content was 54.0 mol%. The major fatty acid was iso-C17 : 0 and the major menaquinone was MK-9(H2). The cell wall contained glutamic acid, serine, alanine and ornithine in a molar ratio of 1.00 : 1.07 : 2.64 : 0.83. The polar lipids consisted of phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid. Cell-wall sugars were rhamnose and mannose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SK20-1 T belongs to the class Ktedonobacteria, and that the strain is most closely related to Ktedonobacter racemifer SOSP1-21T (88.5%). On the basis of its phenotypic features and phylogenetic position, we propose that SK20-1T represents a novel genus and species, Thermosporothrix hazakensis gen. nov., sp. nov., within the new family Thermosporotrichaceae fam. nov. The type strain of Thermosporothrix hazakensis is strain SK20-1 T (=JCM 16142T =ATCC BAA-1881T). In addition, we propose an emended description of the class Ktedonobacteria to classify the class in the phylum Chloroflexi. © 2010 IUMS.


Patent
Kennan Eisei Kogyo Co. | Date: 2012-05-31

This invention provides a novel cellulase derived from Thermosporothrix hazakensis. The cellulase derived from Thermosporothrix hazakensis has enzyme activity on at least -glucan, soluble cellulose, crystalline cellulose, phosphoric acid-swollen cellulose, and xylan.


PubMed | Kennan Eisei Kogyo Co.
Type: Journal Article | Journal: International journal of systematic and evolutionary microbiology | Year: 2013

A Gram-negative bacterium, designated CKTN2(T), was isolated from compost. Cells of strain CKTN2(T) were strictly aerobic rods. The isolate grew at 20-50 C (optimum 40-45 C), but not below 15 C or above 52 C, and at pH 5.9-8.8 (optimum pH 7.0), but not below pH 5.4 or above pH 9.3. The DNA G+C content was 40.3 mol%. The predominant menaquinone was MK-7. The major fatty acids were iso-C15:0 (45.2%), iso-C17:0 3-OH (11.1%) and C18:0 (14.5%). Analysis of the 16S rRNA gene sequence of strain CKTN2(T) revealed that it is a member of the genus Sphingobacterium and is most closely related to Sphingobacterium alimentarium DSM 22362(T) (93.2% 16S rRNA gene sequence similarity). Strain CKTN2(T) could be distinguished from its closest phylogenetic relatives by different phenotypic characteristics. According to the phenotypic and genotypic characteristics, strain CKTN2(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium thermophilum sp. nov. is proposed. The type strain is CKTN2(T) (=JCM 17858(T) =KCTC 23708(T)).

Loading Kennan Eisei Kogyo Co. collaborators
Loading Kennan Eisei Kogyo Co. collaborators