Kemin Industries Zhuhai Co.

Zhuhai, China

Kemin Industries Zhuhai Co.

Zhuhai, China
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Liu W.,Chinese Academy of Fishery Sciences | Wu J.P.,Chinese Academy of Fishery Sciences | Li Z.,Kemin Industries Zhuhai Co. | Duan Z.Y.,Kemin Industries Zhuhai Co. | Wen H.,Chinese Academy of Fishery Sciences
Aquaculture Nutrition | Year: 2017

This study was conducted to investigate the effects of dietary protease on growth performance, feed utilization, whole-body proximate composition, nutrient digestibility, intestinal and hepatopancreas structure of juvenile Gibel carp, Carassius auratus gibelio (mean weight 8.08 ± 0.18 g). Six diets were prepared, including a positive control diet (dietary protein 350 g/kg, PC), one negative control diet (dietary protein 33 g/kg, NC) and four protease supplementations diets, which were 75, 150, 300 and 600 mg/kg protease NC diet. After 12 weeks of diet feeding in indoor recycle aquarium tanks, no significant difference (p > .05) was found on growth performance between fish fed diet with 75-600 mg/kg protease and the PC group. Compared with the fish fed the NC diet, the specific growth rate of fish fed 300 mg/kg protease increased significantly (p < .05), as well as protein efficiency ratios (p < .05), while feed conversion was the opposite (p < .05). The nutrient digestibility of crude protein and lipid was higher (p < .05) in fish fed 150 mg/kg protease diet than the PC diet. Whole-body proximate composition of fish was not affected (p > .05) by the dietary treatment. Serum alkaline phosphatase and albumin were significantly affected by dietary protease (p < .05), while the content of total protein, glucose, triglyceride, total cholesterol, aspartate aminotransferase and alanine aminotransferase activities in serum was not affected (p > .05). Foregut muscular thickness was thinner (p < .05), when the fish fed diets supplementation of protease in 150 or 600 mg/kg diet than the NC diet. Protease activities in hepatopancreas and foregut were higher (p < .05), in the fish fed 150 or 300 mg/kg protease diet than the fish fed the PC diet, but those in the mid- and hindgut were not significantly affected (p > .05) by the dietary treatments. Based on the regression analysis of weight gain rate, the optimal dietary inclusion level of protease was 400 mg/kg in the diet for juvenile Carassius auratus gibelio. © 2017 John Wiley & Sons Ltd.

Samuel K.G.,Chinese Academy of Agricultural Sciences | Wang J.,Chinese Academy of Agricultural Sciences | Yue H.Y.,Chinese Academy of Agricultural Sciences | Wu S.G.,Chinese Academy of Agricultural Sciences | And 3 more authors.
Poultry Science | Year: 2017

Gallic acid (GA), widely distributed in plants and feeds, is known to have a diverse range of activities such as anti-oxidant, anti-inflammatory, antibacterial, anti-allergic, anti-mutagenic, and anticarcinogenic. The purpose of this study was to investigate the efficacy of inclusion of dietary GA at levels 0, 25, 50, 75, 100, or 150 mg/kg on growth performance, antioxidant status, and jejunum intestinal morphology of broiler chicks. In total, 630 one-day-old Arbor Acres (AA) male broiler chicks were randomly allotted to 6 treatment groups for a period of 6 weeks. The results indicate that dietary addition of GA at 75 to 100 mg/kg improved feed conversion efficiency in both the grower (d 21 to 42, P = 0.045) and overall (d 1 to 42, P = 0.026) periods. Dietary addition of GA at a concentration ≥100 mg/kg was able to exhibit higher breast muscle ratio at 42 d (P = 0.043). Interestingly, dietary GA inclusion level from 50 to 100 mg/kg reduced the crypt depth (P = 0.009) and increased the villus height:crypt depth ratio (VCR) of the birds (P = 0.006). Dietary supplementation of GA at 100 mg/kg decreased plasma malondialdehyde (MDA) content at 42 d of age (P = 0.030). Moreover, dietary addition of GA linearly increased plasma total antioxidant capacity (P = 0.039) and plasma total superoxide dismutase activities (P = 0.049) at 21 d of age. However, analysis of plasma biochemical markers revealed that dietary supplementation of GA did not exhibit beneficial health effects. Overall, we conclude that 75 to 100 mg/kg of GA are suitable for enhanced growth performance and health benefits in a broiler diet. © 2017 Poultry Science Association Inc.

Yu G.,Sichuan Agricultural University | Chen D.,Sichuan Agricultural University | Yu B.,Sichuan Agricultural University | He J.,Sichuan Agricultural University | And 6 more authors.
Animal Feed Science and Technology | Year: 2016

The objective of this study was to determine the effect of a coated protease on apparent (AID) and standardized (SID) ileal digestibility of crude protein (CP) and amino acids (AA) in weaned piglets. Eighteen barrows with initial body weight (BW) of 13.5. ±. 0.2. kg were randomly allotted to 3 diets (control, protease diet, and nitrogen-free diet) with 6 piglets per diet after fitting with a T-cannula in the distal ileum. Control and protease diets were corn-soybean meal based diet supplemented with or without 200. mg/kg of coated protease. The nitrogen-free diet was used to measure basal endogenous losses of AA. The experiment lasted for 7 days, and ileal digesta were collected on days 6 and 7. Results showed that the coefficients of AID and SID of CP and total AA were increased by protease supplementation (P <. 0.05). For indispensable AA, the coefficients of AID of Ile, Lys, Met and Thr, and the coefficients of SID of Ile, Lys, Met, Thr and Trp were increased by protease (P <. 0.05). For dispensable AA, both the coefficients of AID and SID of Ala, Asp, Cys, Gly and Ser were increased by protease (P <. 0.05). In conclusion, dietary supplementation with coated protease increases coefficients of AID and SID of CP and most AAs in weaned piglets. © 2016 Elsevier B.V.

Zhang B.,China Agricultural University | Haitao L.,Kemin Industries Zhuhai Co. | Zhao D.,China Agricultural University | Guo Y.,China Agricultural University | Barri A.,Kemin Agrifoods North America
Animal Feed Science and Technology | Year: 2011

A completely randomized design study with a 3 × 2 factorial arrangement was conducted to evaluate the effects of three different fat sources (soybean oil, tallow, and poultry fat) with or without emulsifier supplementation on performance, coefficient of total tract apparent digestibility (CTTAD) of fatty acids, and apparent metabolizable energy (AME) content in broiler chickens. Two hundred and fifty-two one-day-old male Arbor Acres broiler chickens were randomly divided into 6 different treatments: (T1) basal diet containing soybean oil without lysophosphatidylcholine (LPC) supplementation, (T2) basal diet containing soybean oil with LPC supplementation, (T3) basal diet containing tallow without LPC supplementation, (T4) basal diet containing tallow with LPC supplementation, (T5) basal diet containing poultry fat without LPC supplementation, and (T6) basal diet containing poultry fat with LPC supplementation. Body weight gains from broiler chicks fed diets containing tallow were lower (P<0.05) than the body weight gains from chicks that were fed diets containing soybean oil or poultry fat in both the starter and grower periods. Birds fed diets containing tallow had the highest FCR (P<0.05), followed by the birds that were fed diets containing poultry fat, and soybean oil. The CTTAD of C16:0, C18:2, and C18:3n3 was greater (P<0.05) for broilers fed diets containing soybean oil than for those fed diets containing tallow or poultry fat in the starter period. The addition of LPC increased (P<0.05) body weight gain of broiler chickens in the starter period and the AME of the diets in the grower period, and tended to reduce FCR (P=0.072) in the starter period. LPC supplementation increased (P<0.05) the CTTAD of C16:0, C18:1n7 and C18:1n9 in the starter period, and of C18:2, and C18:3n3 in the grower period (P<0.05). There were no significant interactions between fat sources and the addition of LPC. These data indicated that LPC supplementation can improve body weight gain of broiler chickens in the starter period. This effect may be associated with an increase of CTTAD of FA due to LPC activity. © 2010.

Wang Y.,CAS South China Sea Fisheries Research Institute | Li Z.,Kemin Industries Zhuhai Co. | Li J.,Chinese Academy of Fishery Sciences | Niu J.,CAS South China Sea Fisheries Research Institute | And 3 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2013

Intensively-reared white shrimp Litopenaeus vannamei are subjected to various environmental stresses that can have severe effects on their physiology. For example, low salinity stress decreases the activity of antioxidant enzymes. How to reduce the adverse effects of multiple environmental stresses on shrimp has become a new challenge in aquaculture. Chlorogenic acid has been documented to effectively scavenge free radicals and improve antioxidant defenses in humans and animals. The objectives of this study were to evaluate the effects of chlorogenic acid on the antioxidant system of L. vannamei, and determine whether it protects the shrimp against low salinity stress. A total of 360 shrimps with an average initial body weight of about 6.7 g were divided randomly into four replicated (×3) treatments, and reared indoors in 500-L glass-fiber tanks containing 300 L of natural seawater. The shrimp were fed one of four diets, a control diet without chlorogenic acid, and three diets containing different levels (100, 200, 400 mg/ kg diet) of chlorogenic acid. Shrimps in each treatment were hand-fed one of the four diets to apparent satiation three times daily; uneaten food and feces were removed by siphoning. Prior to the beginning of the feeding trial, all shrimps were acclimatized for one week. Each tank was continuously oxygenated using an air pump, and 30% of the seawater was renewed daily. During the experimental period, water temperature, salinity, pH and other water quality parameters were kept stable. After being fed for 28 days, all shrimps were subjected to acute low salinity stress in which salinity was decreased rapidly from 32 to 10 for 72 h. The results indicated that at normal salinity (32), there were no significant differences in the survival, the total antioxidant status (T- AOC), superoxide dismutase (SOD) and catalase (CAT) activities of the hemolymph of white shrimps that were fed diets with or without chlorogenic acid. However, the glutathione peroxidase (GPx) activity and gene expression of GPx and CAT of shrimp hemolymph increased significantly (P < 0.05) when shrimps were, for 14 days, fed diets containing different concentrations of chlorogenic acid. After exposure to low-salinity seawater for 24 h, survival of shrimps fed diets supplemented with chlorogenic acid increased by 10% compared with the control treatment, although the difference between the control and treatments was not significant (P>0.05). Activities of T-AOC, SOD and GPx of shrimp hemolymph in all treatments increased significantly after 24 h of low salinity stress, which suggests that low salinity induced an antioxidant stress response in the shrimps. Moreover, GPx and CAT activities of hemolymph in shrimp fed diets supplemented with chlorogenic acid were significantly higher than those fed the control diet after 24 h of low salinity stress (P<0.05). Over a period of 72 h of exposure to low salinity, activities of T-AOC, GPx and CAT, along with gene expression of GPx and CAT of hemolymph in white shrimp fed dietary chlorogenic acid, were significantly higher than those measured in the control animals. In conclusion, the findings of this study suggest that chlorogenic acid can serve as an effective antioxidant to regulate the antioxidant defense system of white shrimp and improve resistance against low salinity stress.

Wang Y.,Key Laboratory of Aquatic Product Processing | Li Z.,Kemin Industries Zhuhai Co. | Li J.,Chinese Academy of Fishery Sciences | Duan Y.-F.,Key Laboratory of Aquatic Product Processing | And 4 more authors.
Fish and Shellfish Immunology | Year: 2015

An eight-week feeding trial followed by an acute combined stress test of low-salinity and nitrite were performed to evaluate effects of chlorogenic acid (CGA) on growth performance and antioxidant capacity of white shrimp Litopenaeus vannamei. Shrimp were randomly allocated in 12 tanks (30 shrimp per tank) and triplicate tanks were fed with a control diet or diets containing different levels of CGA (100, 200 and 400mgkg-1 feed) as treatment groups. Growth performance including weight gain (WG), biomass gain (BG), feed conversion ratio (FCR), and feed intake were determined after feeding for 56 days. Antioxidant capacity were evaluated by determining the activity of total antioxidant status (TAS), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) as well as the gene expression of GSH-Px and CAT in the hepatopancreas of shrimp at the end of feeding trial and again at the end of the combined stress test. The results indicated that supplemention of CGA had no significant effects on the growth performance and the activities of TAS, SOD, GSH-Px and CAT in hepatopancreas of shrimp cultured under normal conditions for 56 days. However, compared with the control group, CGA (200, 400mgkg-1 feed) significantly improved the resistance of L.vannamei against the combined stress of low-salinity and nitrite, as indicated by the significant (P<0.05) higher survival, higher activities of TAS, GSH-Px and CAT, as well as higher transcript levels of GPx and CAT gene in shrimp treated with CGA in the combined tress test. Our findings suggested that CGA possessed dual-modulatory effects on antioxidant capacity of L.vannamei and could be a potential feed additive that can enhance shrimp resistance against environmental stresses. The recommended application dosage is 200mgkg-1 and further studies are needed to clarify the action model of CGA efficiency. © 2015 Elsevier Ltd.

PubMed | Kemin Industries Zhuhai Co. and China Agricultural University
Type: Journal Article | Journal: Asian-Australasian journal of animal sciences | Year: 2016

Two experiments were conducted to evaluate effects of coated compound proteases (CC protease) on apparent total tract digestibility (ATTD) of nitrogen (N) and energy, and apparent ileal digestibility (AID) of amino acids (AA) and nutrients in diets for pigs. In Exp. 1, 12 crossbred barrows (initial body weight: 20.141.71 kg) were housed in individual metabolism crates and allotted into 2 treatments with 6 piglets per treatment according to weight in a randomized complete block design. The 2 diets were corn-soybean meal basal diets with (0.2 g/kg) or without CC protease supplementation. The CC protease supplementation increased (p<0.05) the digestible and metabolizable N and energy values and the digestibility and retention rate of N in the diet. The ATTD of energy and nutrients had been improved (p<0.05) in the diet supplemented with CC protease. In Exp. 2, 12 crossbred barrows (initial body weight: 20.791.94 kg), fitted with T-cannulas at the distal ileum, were blocked by body weight into 2 groups with 6 pigs each. The diets were the same as those in Exp. 1. The CC protease increased (p<0.05) the AID of crude protein and some essential AA including arginine, isoleucine and leucine. The AID and ATTD of energy and nutrients had been improved (p<0.05) by supplemental CC protease, but the hindgut digestibility of nutrients was unaffected. Overall, the CC protease improved the ATTD of N and energy and AID of some indispensible AA and nutrients in the corn-soybean meal diet for pigs. Therefore, the CC protease supplement could improve the utilization of protein in the corn-soybean meal diet and thus contribute to lower N excretion to the environment.

Peng Q.Y.,Sun Yat Sen University | Peng Q.Y.,Kemin Industries Zhuhai Co. | Li J.D.,Liaoning University | Li Z.,Kemin Industries Zhuhai Co. | And 2 more authors.
Animal Feed Science and Technology | Year: 2016

This study was conducted to investigate the effects of diets containing oregano essential oil (OEO) on growth performance, carcass traits and intestinal morphology of broilers during a 42-day production period. A total of 448 one-day-old Arbor Acres broilers were randomly distributed into 4 dietary treatments with 7 replicate pens per treatment and 16 birds per pen. The dietary treatments were as follows: (1) corn-soybean basal diet (control, CON), (2) CON + 8 mg/kg avilamycin (AVI), (3) CON + 300 mg/kg OEO preparation (OEO300), and (4) CON + 600 mg/kg OEO preparation (OEO600). The results showed that both OEO300 and OEO600 groups increased (P = 0.007) the final body weight compared to the CON group. On day 21, OEO600 increased average daily gain (ADG) and lowered feed conversion ratio (both P < 0.05) compared to the CON. On day 42, both OEO300 and OEO600 groups increased (P < 0.05) ADG and average daily feed intake (ADFI) compared to the CON. Moreover, OEO600 increased (P < 0.05) ADG and ADFI compared with AVI. Carcass parameters were similar among avilamycin and OEO preparation treatments. Avilamycin and OEO supplementation improved (P < 0.05) the dressing percentage and eviscerated rate compared with CON. The broilers fed with OEO600 had the highest (P = 0.003) breast muscle percentage, and the lowest (P = 0.008) abdominal fat percentage. All treatments had no effect on the villus height in the jejunum of broilers. However, OEO300 and OEO600 decreased (P = 0.000) the crypt depth and increased (P = 0.000) the villus height to crypt depth ratio compared with CON and AVI, which may contribute to the improvement of growth performance. In conclusion, OEO supplementation exhibited a significantly positive effect on the growth performance, carcass traits and intestinal health of broilers, indicating that OEO may be a promising alternative to antibiotic growth promoters. © 2016 Elsevier B.V.

Liu H.,Guangdong Ocean University | Li Z.,Kemin Industries Zhuhai Co. | Tan B.,Guangdong Ocean University | Lao Y.,Kemin Industries Zhuhai Co. | And 3 more authors.
Fish and Shellfish Immunology | Year: 2014

The common pathogens in aquaculture are very different from those in terrestrial animals. The objective of this study was to isolate probiotic strain (s) from the digestive tract of healthy white shrimp Litopenaeus vannamei which was effective against aquatic animal pathogens. The putative probiotic strain S12 was identified as Bacillus subtilis based on the morphological and biochemical properties and 16S rDNA gene sequencing. The L. vannamei were fed with five different diets: control (basal diet with no probiotics or antibiotics), antibiotic control (basal diet supplemented with 0.3% florfenicol), basal diet supplemented with 5 × 109 cfu kg-1 5 × 1010 cfu kg-1 and 5 × 1011 cfu kg-1 probiotic S12 (PS1-3). Each diet was randomly fed to quadruplication groups of 40 shrimps (0.4 ± 0.01 g) reared in tanks. After an 8-week feeding, the survival rate of shrimps fed with PS1 and PS3 were the highest among all treatments (P < 0.05). The moisture content of shrimps fed with florfenicol was significantly lower than that of the control group (P < 0.05). The supplement of probiotic S12 decreased the body crude lipid significantly (P < 0.05). The activities of phagocytic rate, lysozyme (LZ), superoxide dismutase phenoloxidase (SOD) and antibacterial activity were significantly higher than those in the control (P < 0.05), and the activities of SOD and the antibacterial activity in PS2 and PS3 were significantly higher than those in antibiotic control (P < 0.05). When infected with Vibrio harveyi at 4-weeks, the mortality was significantly lower (P < 0.05) in PS2 and PS3 groups than that in the control. After being infected with V. harveyi at 8-weeks, the mortality was significantly lower in the probiotic and antibiotic groups than that in the control (P < 0.05). This study suggested that probiotics could be used as an effective immunopotentiator, the optimal dose of the probiotic strain S12 is 5 × 1010 cfu kg-1 diet. © 2014 Elsevier Ltd.

PubMed | Kemin Industries Zhuhai Co., Zhejiang University of Technology and Zhejiang University
Type: Journal Article | Journal: Journal of dairy science | Year: 2014

This experiment was conducted to determine the influences of supplementing different levels of an additive containing lutein in the diet of Chinese Holstein lactating cows on production performance, antioxidative plasma metabolites, and milk quality. This study was performed on 60 multiparous Holstein dairy cows in peak lactation. The cows were randomly allocated to 1 of 4 homogeneous treatments, with lutein preparation (extracted from marigolds; effective lutein content was 2%) added at levels of 0, 100, 150, and 200 g/d per head, with the actual available amounts being 0, 2, 3, and 4 g of lutein/d per head, respectively. The experiment lasted for 13 wk, with the first week for adaptation. Milk yield and milk compositions were recorded weekly, and milk concentrations of lutein, dry matter intake, and antioxidative blood index were analyzed in the first, fourth, seventh, and thirteenth week of the study. The results showed that adding lutein in the diet had no effect on dry matter intake compared with the control group; however, it slowed down the trend of decline in milk yield, and had a linear incremental effect on milk yield with increasing concentration of lutein. Dietary lutein tended to quadratically increase the percentage of milk fat, and linearly increased milk lactose concentration, with the highest value when treated at 200 g of lutein preparation/d per head, and decreased somatic cell count, with the lowest values when treated with 150 and 200 g of lutein preparation/d per head. The concentration of lutein in milk linearly increased with the incorporation of the additive, with a value of 0.59, 0.70, 1.20, and 1.50 g/100mL when treated with 0, 100, 150, and 200 g/d, respectively. Total plasma antioxidant capacity tended to linearly increase in cows fed lutein preparation, whereas plasma superoxide dismutase and glutathione peroxidase activities did not differ significantly. In conclusion, addition of lutein in the diet could improve the production performance and health status of dairy cows.

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