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Tsidulko A.Y.,Karolinska Institutet | Matskova L.,Karolinska Institutet | Astakhova L.A.,Kemerovo Institute of Food Science and Technology | Ernberg I.,Karolinska Institutet | Grigorieva E.V.,Karolinska Institutet
Oncotarget | Year: 2015

The involvement of proteoglycans (PGs) in EBV-host interactions and lymphomagenesis remains poorly investigated. In this study, expression of major proteoglycans (syndecan-1, glypican-1, perlecan, versican, brevican, aggrecan, NG2, serglycin, decorin, biglycan, lumican, CD44), heparan sulphate (HS) metabolic system (EXT1/2, NDST1/2, GLCE, HS2ST1, HS3ST1/2, HS6ST1/2, SULF1/2, HPSE) and extracellular matrix (ECM) components (collagen 1A1, fibronectin, elastin) in primary B cells and EBV carrying cell lines with different phenotypes, patterns of EBV-host cell interaction and viral latency stages (type I-III) was investigated. Primary B cells expressed a wide repertoire of PGs (dominated by serglycin and CD44) and ECM components. Lymphoblastoid EBV+ B cell lines (LCLs) showed specific PG expression with down-regulation of CD44 and ECM components and up-regulation of serglycin and perlecan/HSPG2. For Burkitt's lymphoma cells (BL), serglycin was down-regulated in BL type III cells and perlecan in type I BL cells. The biosynthetic machinery for HS was active in all cell lines, with some tendency to be down-regulated in BL cells. 5'-aza-dC and/or Trichostatin A resulted in transcriptional upregulation of the genes, suggesting that low expression of ECM components, proteoglycan core proteins and HS biosynthetic system is due to epigenetic suppression in type I cells. Taken together, our data show that proteoglycans are expressed in primary B lymphocytes whereas they are not or only partly expressed in EBV-carrying cell lines, depending on their latency type program. Source


Esina Z.N.,Kemerovo State University | Miroshnikov A.M.,Kemerovo Institute of Food Science and Technology | Korchuganova M.R.,Kemerovo State University
Journal of Physical Chemistry B | Year: 2015

The flash point is one of the most important characteristics used to assess the ignition hazard of mixtures of flammable liquids. To determine the flash points of mixtures of secondary alcohols with n-alkanes, it is necessary to calculate the activity coefficients. In this paper, we use a model that allows us to obtain enthalpy of fusion and enthalpy of vaporization data of the pure components to calculate the liquid-solid equilibrium (LSE) and vapor-liquid equilibrium (VLE). Enthalpy of fusion and enthalpy of vaporization data of secondary alcohols in the literature are limited; thus, the prediction of these characteristics was performed using the method of thermodynamic similarity. Additionally, the empirical models provided the critical temperatures and boiling temperatures of the secondary alcohols. The modeled melting enthalpy and enthalpy of vaporization as well as the calculated LSE and VLE flash points were determined for the secondary alcohol and n-alkane mixtures. © 2015 American Chemical Society. Source


Prosekov A.,Kemerovo Institute of Food Science and Technology | Ulrikh E.,Institute of Higher Education in Food Sciences | Dishluk L.,Institute of Higher Education in Food Sciences | Babich O.,Kemerovo Institute of Food Science and Technology
Biology and Medicine | Year: 2015

This article investigates the chemical resistance of films and capsules based on vegetable analogs of pharmaceutical gelatin and estimates the chemical resistance of films and capsules per hour for the final destruction of structures in aggressive environments. It has been established that the greatest dissolution rate of films and capsules based on vegetable analogs of pharmaceutical gelatin is in concentrated hydrochloric acid, which to some extent reproduces the acidic environment of the stomach. The chemical resistance of films and capsules based on vegetable analogs of pharmaceutical gelatin is also associated with the ability of the filler to be wetted by the aggressive environment. It has been established that the best wetting of films and capsules occurs also with concentrated hydrochloric acid. The impact of hydrochloric acid leads to the loosening of the polymer, to a decrease in its density, and consequently to rapid degradation and dissolution. It is proved that the air cavities and microcracks facilitate migration of the fluid into the film or capsule, which reduces the intermolecular interactions and reduces the strength characteristics of the material. Source


Ivanova S.A.,Kemerovo Institute of Food Science and Technology | Pavsky V.A.,Kemerovo Institute of Food Science and Technology
Theoretical Foundations of Chemical Engineering | Year: 2014

A stochastic model studying the process of formation and destruction of protein gas-liquid disperse system (foam) is proposed. The model allows one to describe the process state at each time moment of the first cycle. Mathematical expectation, variance, and other moments of random values, which characterize the number of bubbles per unit of volume, and the function that describes the rate of foam destruction, are proposed as a basis to calculate the efficiency parameters of the foaming process. The model could be useful for the imitation modeling of the foaming process. © 2014, Pleiades Publishing, Ltd. Source


Prosekov A.Y.,Kemerovo State University | Dyshlyuk L.S.,Kemerovo Institute of Food Science and Technology | Milentieva I.S.,Kemerovo Institute of Food Science and Technology | Sukhikh S.A.,Kemerovo Institute of Food Science and Technology | And 5 more authors.
International Journal of Pharmacy and Technology | Year: 2016

Objective: to study the biocompatibility of 17 strains of lactic acid bacteria previously isolated from the gastrointestinal tract of healthy people and people with oncological diseases. Methods: Lactic acid bacteria obtained from the digestive tract of healthy people and people with oncological diseases sick people from different age groups served as the basis of composing symbiotic consortia with a view to expand their effect. In vitro studies proved their inhibitory effect against lymphoma cell cultures, prostate cancer, breast cancer, hepatocellular carcinoma, brain cancer and pancreas cancer. Results: The paper established that biocompatibility of all group strains is characteristic of the Bifidobacterium breve and Lactobacillus casei strains, conditional biocompatibility can be identified in Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus paracasei, respectively for the first and second groups. Biological incompatibility was observed in combinations: Bifidobacterium bifidum + Micrococcus spp.; Lactobacillus salivarius + Micrococcus spp. Conclusions: Study of antitumor properties of lactobacilli consortia composed of 17 previously selected probiotic strains demonstrate high antitumor activity of these consortia to cancer cell lines of Burkitts lymphoma (BRL2), human prostate cancer DU 145, human breast cancer MDAMB-231 and MCF7, hepatocellular carcinoma HepG2, brain cancer U-87, human pancreatic cancer PANC-1. © 2016, International Journal of Pharmacy and Technology. All rights reserved. Source

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