Rosario Del Tala, Argentina
Rosario Del Tala, Argentina
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Ravasi P.,National University of Rosario | Braia M.,National University of Rosario | Eberhardt F.,National University of Rosario | Elena C.,Keclon Inc. | And 7 more authors.
Journal of Biotechnology | Year: 2015

Enzymatic oil degumming (removal of phospholipids) using phospholipase C (PLC) is a well-established and environmentally friendly process for vegetable oil refining. In this work, we report the production of recombinant Bacillus cereus PLC in Corynebacterium glutamicum ATCC 13869 in a high cell density fermentation process and its performance in soybean oil degumming. A final concentration of 5.5. g/L of the recombinant enzyme was achieved when the respective gene was expressed from the tac promoter in a semi-defined medium. After treatment with trypsin to cleave the propeptide, the mature enzyme completely hydrolyzed phosphatidylcholine and phosphatidylethanolamine, which represent 70% of the phospholipids present in soybean oil. The results presented here show the feasibility of using B. cereus PLC for oil degumming and provide a manufacturing process for the cost effective production of this enzyme. © 2015 Elsevier B.V.


PubMed | National University of Rosario and Keclon Inc.
Type: | Journal: Journal of biotechnology | Year: 2015

Enzymatic oil degumming (removal of phospholipids) using phospholipase C (PLC) is a well-established and environmentally friendly process for vegetable oil refining. In this work, we report the production of recombinant Bacillus cereus PLC in Corynebacterium glutamicum ATCC 13869 in a high cell density fermentation process and its performance in soybean oil degumming. A final concentration of 5.5g/L of the recombinant enzyme was achieved when the respective gene was expressed from the tac promoter in a semi-defined medium. After treatment with trypsin to cleave the propeptide, the mature enzyme completely hydrolyzed phosphatidylcholine and phosphatidylethanolamine, which represent 70% of the phospholipids present in soybean oil. The results presented here show the feasibility of using B. cereus PLC for oil degumming and provide a manufacturing process for the cost effective production of this enzyme.


Keclon Inc. | Entity website

Keclon es una empresa argentina de biotecnologa dedicada al desarrollo de nuevas enzimas para la produccin de aceites y biodiesel. Nuestra plataforma exclusiva nos permite generar rpidamente enzimas nuevas y mejoradas que pueden reducir significativamente costos de produccin utilizando herramientas de ingeniera gentica, biologa sinttica y tcnicas de evolucin dirigida ...


Keclon Inc. | Entity website

Llamado a Licitacin- KECLON S.A ...


Keclon Inc. | Entity website

Contacto menzella@keclon.com0341-153304478 peiru@keclon ...


Keclon Inc. | Entity website

Fundadores Keclon - Todos los derechos reservados - 2012 Rosario, Santa Fe, Argentina


Keclon Inc. | Entity website

Keclon - Todos los derechos reservados - 2012 Rosario, Santa Fe, Argentina


Keclon Inc. | Entity website

Directores Keclon - Todos los derechos reservados - 2012 Rosario, Santa Fe, Argentina


Keclon Inc. | Entity website

Fundadores Keclon - Todos los derechos reservados - 2012 Rosario, Santa Fe, Argentina

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