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Pavan Kumar D.,Kcreddy Institute Of Pharmaceutical Science | Bala Swathi S.,P.A. College
Asian Journal of Pharmaceutical and Clinical Research

Transdermal delivery system bypass the hepatic first pass metabolism and avoid drug degradation due to gastrointestinal pH, enzymes etc., minimize plasma level fluctuations and extend the drug activity besides improving patient compliance. Transdermal films of metoprolol tartarate were prepared using polymers such as ethyl cellulose, poly vinyl alcohol,hydroxy propyl methyl cellulose(HPMC), eudragit S 100. Triethyl citrate was used as plasticizer.The study was undertaken to report the film forming properties of polymers used and in vitro drug release from the prepared monolithic matrices. Effect of drug loading on the drug release rate was also studied. The transdermal films were prepared using solvent casting method. These films were evaluated for Thickness, Percent moisture loss, Percent moisture absorption, Drug content, Weight variation and Folding endurance. In-vitro drug release kinetics was studied using Franz-diffusion cell. Drug release followed zero order kinetics. Drug loading at different concentrations found to have less effect on the film forming properties of the constituent polymers. Results have shown enhanced flux per unit time across rat skin. In conclusion combination of ethyl cellulose, poly vinyl alcohol,hydroxyl propyl methyl cellulose(HPMC), eudragit S100 and triethyl citrate can potentially be optimized to develop an effective transdermal drug delivery system for metoprolol tartarate. Source

Kalaichelvi R.,Kcreddy Institute Of Pharmaceutical Science | Srinivasa Rao D.,Kcreddy Institute Of Pharmaceutical Science
E-Journal of Chemistry

A rapid, simple and validated reversed-phase high-performance liquid chromatographic method has been developed for analysis of aripiprazole in tablet dosage form. Aripiprazole was separated on an ODS analytical column with a 40:60 (v/v) mixture of acetonitrile and triethanolamine buffer (5 mM, pH 3.5 ± 0.05 adjusted by addition of 85% phosphoric acid) as mobile phase at a flow rate of 1.5 mL -1. The effluent was monitored by UV detection at 254 nm. Calibration plots were linear in the range of 20 to 60 μg mL-1 and the LOD and LOQ were 0.411 and 1.248 μg mL-1, respectively. The high recovery and low relative standard deviation confirm the suitability of the method for routine quality control determination of aripiprazole in tablets. Source

Kumar D.P.,Kcreddy Institute Of Pharmaceutical Science | Rao D.S.,Kcreddy Institute Of Pharmaceutical Science
Journal of Chemical and Pharmaceutical Sciences

A simple, sensitive and specific liquid chromatography¡Velectrospray ionization-mass spectrometry method was developed for the quantitative determination of Oxolamine phosphate in human plasma. Sample preparation involved simple liquid¡Vliquid extraction. Bromhexine was used as internal standard. The separation of the analyte, internal standard and possible endogenous compounds were accomplished on a Shim-pack ODS column (150 mm×4.6 mm i.d., 5 μm) with methanol-Vwater (98:2, v/v) as mobile phase. A Turbo-Ion spray source was interfaced between the HPLC and triple quadrupole mass spectrometer (MDS Sciex API 4000). The precursor-product ion m/z was 246.3 → 86.1 m/z and 377.3 → 263.9 m/z were used for quantification of an analyte and its IS. The method was validated over the range of 0.5-V60 ng/mL and the results were acceptable. The method could offer the advantages of shorter run time (5.0 min) and lower LLOQ (0.5 ng/mL) with a decreased plasma volume requirement (250μL). Source

Praveen Kumar D.,Kcreddy Institute Of Pharmaceutical Science | Srinivasa Rao D.,Kcreddy Institute Of Pharmaceutical Science
Journal of Chemical and Pharmaceutical Sciences

The validated protein precipitation method was applied for estimation of Valsartan (VL) in rat plasma with VLD9 as an internal standard (ISTD) by using HPLC-ESI-MS/MS. The chromatographic separation was achieved with 0.1% formic acid in combination with methanol (25:75 v/v) using the C18 column Ascentis Express (50 mm × 4.6 mm, 2.7 μm). The total analysis time was 3 min and flow rate was set to 0.6 ml/min. The mass transitions of VL, VLD9 obtained were m/z 436.3→234.9 and m/z 445.3→234.9. The standard curve shows correlation coefficient (r2) greater than 0.9993 with a range of 5-10000 ng/ml using the linear regression model. The validated method was applied to bioavailability study of ten male wistar rats through intravenous administration of 1.88 mg/kg of body weight of test formulation. Source

Pavan Kumar A.,Kcreddy Institute Of Pharmaceutical Science | Kalaichelvi R.,Kcreddy Institute Of Pharmaceutical Science
Journal of Chemical and Pharmaceutical Sciences

Valsartan is an angiotensin-receptor blocker (ARB) that may be used to treat a variety of cardiac conditions. The analytical method was developed by studying different parameters. First of all, maximum absorbance was found to be at 229nm and the peak purity was excellent. Injection volume was selected to be 20μ l which gave a good peak area. The column used for study was Inertsil C18. The percent recovery was found to be 98.0-101.50 was linear and precise over the same range. Both system and method precision was found to be accurate and well within range. Source

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