Kbinstitute Of Pharmaceutical Education And Research

Ghandinagar, India

Kbinstitute Of Pharmaceutical Education And Research

Ghandinagar, India
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Shah G.H.,Gujarat University | Patel B.G.,Gujarat University | Shah G.B.,Kbinstitute Of Pharmaceutical Education And Research
Asian Journal of Pharmaceutical and Clinical Research | Year: 2017

Objective: The objective of the present work is to develop carbon tetrachloride (CCl4)-induced chronic hepatotoxicity model in rats and its application in evaluation of hepatoprotective activity of silymarin. Methods: Animals were divided into four groups. Three groups were the disease induction group and 4th was the treatment group. In disease induction groups, chronic liver injury was induced by administration of CCl4 through intraperitoneal route (1 ml/kg) for 7-8 weeks. For treatment Group, 1 ml silymarin suspension (orally) and CCl4 was given for 7-8 weeks. During disease induction and treatment period (7-8 weeks), blood samples were collected and serum was separated which in turn used to analyze liver function tests such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphate (ALP), direct bilirubin, total protein (TP), and albumin (Alb) levels. Along with liver functional tests, tests to check cholesterol, glucose, and malondialdehyde (MDA) were also performed. Liver fibrosis and cirrhosis was quantified by histopathological studies of small portion of the excised liver. Model was validated by repetition of the experiment. Intermediate dissection was carried out to measure an extent of liver damage. Result: Serum SGOT, SGPT, ALP, and direct bilirubin were found to be significantly higher in CCl4 intoxicated rats. TP and Alb were decreased, and MDA was found to be significantly higher in CCl4 intoxicated rats, which is the main end product of lipid peroxidation. Whereas in the treatment group silymarin improved the liver functions in CCl4 toxicated drug. Conclusion: We conclude that protein oxidation may play a role in the pathogenesis of CCl4 induced liver injury. The accumulation of oxidized proteins may be an early indication of CCl4 induced liver damage and silymarin found to be effective in liver injury by inhibiting protein oxidation. © 2017 The Authors.


Shah G.B.,Kbinstitute Of Pharmaceutical Education And Research | Chorawala M.C.,Kbinstitute Of Pharmaceutical Education And Research | Trivedi V.R.,Kbinstitute Of Pharmaceutical Education And Research | Deshpande S.S.,Kbinstitute Of Pharmaceutical Education And Research
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

Sucrofer (an Iron Sucrose complex) is one of the widely used Indian preparations for treatment of Iron deficiency anemia. The study was conducted to determine the possible efficacy and safety differences between the innovator's Venofer and Indian similar (Sucrofer). Thirty rats were divided into three groups and assigned to receive Sucrofer, Venofer or isotonic saline solution (control). Five single intravenous doses of iron (40 mg iron/kg) or saline (equivalent volume) were administered every 7 days for 4 weeks. Blood and urine samples were collected for biological assessment prior to sacrifice (day 28) after which kidney, liver, and heart homogenates were collected for determination of antioxidant enzyme levels. Immmunohistochemistry techniques were used to identify tissue ferritin, TNFα and IL-6. Systolic blood pressure, creatinine clearance as well as proteinuria were not significantly altered by sucrofer as compared to Venofer throughout the study. Post 28 days treatment, serum levels of AST, ALT and ALP were also not altered by sucrofer as compared to both vehicle and Venofer group. Liver, heart and kidney catalase, thiobarbituric reactive species, CuZn-superoxide dismutase and glutathione peroxidase activity, and reduced to oxidized glutathione ratio were also not significantly altered by sucrofer as compared to venofer at 4 weeks of treatment. Serum iron and percentage transferrin saturation were elevated in both treatment groups (except control) and no differences in haemoglobin concentration were observed. Liver, kidney and heart TNFα and IL-6 were not significantly altered in both treatment groups as compared to control group on day 28. These findings suggest that both Venofer and Sucrofer have similar safety and efficacy profile.


Patel V.I.,Bs Patel Pharmacy College Linch | Patel V.I.,Kbinstitute Of Pharmaceutical Education And Research | Patel B.,Bs Patel Pharmacy College Linch | Patel B.,Kbinstitute Of Pharmaceutical Education And Research
International Journal of Pharma and Bio Sciences | Year: 2010

The present work deals with evaluation of anti-tubercular activity of various aldehyde derivatives synthesized by Claisen-Schmidt condensation method. The formation of pyrazole derivatives by reaction with phenyl isothiocyanate was also attempted. The synthesized derivatives were screened for anti-tubercular activity and the compounds. All the Compounds characterized on the basis of their IR, MASS, 1H NMR spectroscopic data analysis.

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