Kazan, Russia
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Sitdikova G.F.,Kazan University | Yakovlev A.V.,Kazan University | Odnoshivkina Y.G.,Kazan Medical University | Zefirov A.L.,Kazan Medical University
Neurochemical Journal | Year: 2011

Using electrophysiological and optical methods, we studied the effects of sodium hydrosulfide (NaHS), a hydrogen sulfide donor, on the dynamics of transmitter release and exo- and endocytosis of synaptic vesicles in motor nerve endings during long-term high-frequency stimulation (20 Hz) in experiments with the cutaneous pectoris frog muscle. H2S increased the amplitude of endplate currents under conditions of a single stimulation of the motor nerve and slowed down the depression of the end plate currents during high-frequency stimulation (20 Hz, 3 min). Using the endocytic fluorescent dye FM 1-43, we showed that NaHS increased the dye uptake during high-frequency stimulation as compared to the control. However, after termination of the high-frequency stimulation the fluorescence intensity of nerve terminals was lower in the presence of NaHS than in the control experiments. In addition, NaHS slowed the dye release from the nerve terminals that were loaded during 20 Hz stimulation. The results thus obtained indicate that H2S accelerates the synaptic vesicles cycle in frog motor nerve ending by enhancement of exocytosis and fast endocytosis of synaptic vesicles during high-frequency stimulation. © 2011 Pleiades Publishing, Ltd.


Yakovleva L.A.,Veteran Hospital | Zalyalova Z.A.,Kazan Medical University | Altunbaev R.A.,Kazan Medical University
Zhurnal Nevrologii i Psihiatrii imeni S.S. Korsakova | Year: 2015

Objective. To specify the character of pain syndromes and determine their relationship with main symptoms of ON and OFF periods in patients with complications of long-term treatment with levodopa medications. Materration of treatment with levodopa 6.9±1.2 years (from 5 to 9 years). The severity of disease course, charactial and methods. Authors examined 40 patients with Parkinson’s disease (27 women and 13 men), mean age 69.2±8 years (from 62 to 85 years), illness duration 9.2±1.2 years (from 7 to 11 years), duer and intensity of pain syndrome were assessed. Results and conclusion. We described pain syndromes that had differences in the pathogenesis and localization. Adjustment of the antiparkinsonian treatment resulted in the decrease in their severity. Motor fluctuations and drug-induced dyskinesia that was accompanied by pain sensations were the most frequent signs of Parkinson’s disease during its progression. The pain syndrome was related to main symptoms of the ON and OFF period in patients with complications of long-term treatment with levodopa. The pain fluctuations had both nociceptive and central neuropathic pain phenotypes. © 2015, Media Sphera. All rights reserved.


PubMed | Veteran Hospital and Kazan Medical University
Type: Journal Article | Journal: Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova | Year: 2015

To specify the character of pain syndromes and determine their relationship with main symptoms of ON and OFF periods in patients with complications of long-term treatment with levodopa medications.Authors examined 40 patients with Parkinsons disease (27 women and 13 men), mean age 69.28 years (from 62 to 85 years), illness duration 9.21.2 years (from 7 to 11 years), duration of treatment with levodopa 6.91.2 years (from 5 to 9 years). The severity of disease course, character and intensity of pain syndrome were assessed.We described pain syndromes that had differences in the pathogenesis and localization. Adjustment of the antiparkinsonian treatment resulted in the decrease in their severity. Motor fluctuations and drug-induced dyskinesia that was accompanied by pain sensations were the most frequent signs of Parkinsons disease during its progression. The pain syndrome was related to main symptoms of the ON and OFF period in patients with complications of long-term treatment with levodopa. The pain fluctuations had both nociceptive and central neuropathic pain phenotypes.


Malomouzh A.I.,Russian Academy of Sciences | Nurullin L.F.,Russian Academy of Sciences | Arkhipova S.S.,Russian Academy of Sciences | Nikolsky E.E.,Kazan Medical University
Muscle and Nerve | Year: 2011

In this study we demonstrate expression of the N-methyl-D-aspartate receptor NR1 subunit in the rat neuromuscular junction of skeletal muscles of different functional types (extensor digitorum longus, soleus, and diaphragm muscles) using fluorescence immunocytochemistry. Electron microscopic immunocytochemistry has shown that the NR1 subunit is localized solely on the sarcolemma in the depths of the postsynaptic folds. These findings suggest participation of the glutamatergic signaling system in functioning of the adult mammalian neuromuscular junction. © 2011 Wiley Periodicals, Inc.


Akhmadeev N.R.,Kazan Medical University | Miftahof R.,Arabian Gulf University
International Journal of Design and Nature and Ecodynamics | Year: 2010

A biomechanical model of the human stomach is proposed, that is based on detailed biological data of the structure and function of the organ. The process of electromechanical conjugation and the spread of the electromechanical wave along the stomach wall were analyzed numerically. Results revealed patterns of stress-strain distribution in the organ. Thus the fundus, the body and the antrum of the organ always experience biaxial stress-strain states, while the cardia and the pylorus undergo uniaxial loading. The circular smooth muscle layer produced greater total forces throughout in comparison to the outer longitudinal smooth muscle layer. The body of the organ along the lesser curvature and the cardia-fundus areas were overstressed compare to other regions. Although the theoretical results resemble qualitatively patterns of electrical and mechanical activity observed in vivo and in vitro there is currently no affirmative experimental evidence to provide a detailed quantitative comparison of the results. © 2010 WIT Press.


Petrov A.M.,Kazan Medical University | Kasimov M.R.,Kazan Medical University | Zefirov A.L.,Kazan Medical University
Acta Naturae | Year: 2016

Cholesterol is an important constituent of cell membranes and plays a crucial role in the compartmentalization of the plasma membrane and signaling. Brain cholesterol accounts for a large proportion of the body's total cholesterol, existing in two pools: the plasma membranes of neurons and glial cells and the myelin membranes . Cholesterol has been recently shown to be important for synaptic transmission, and a link between cholesterol metabolism defects and neurodegenerative disorders is now recognized. Many neurodegenerative diseases are characterized by impaired cholesterol turnover in the brain. However, at which stage the cholesterol biosynthetic pathway is perturbed and how this contributes to pathogenesis remains unknown. Cognitive deficits and neurodegeneration may be associated with impaired synaptic transduction. Defects in cholesterol biosynthesis can trigger dysfunction of synaptic transmission. In this review, an overview of cholesterol turnover under physiological and pathological conditions is presented (Huntington's, Niemann-Pick type C diseases, Smith-Lemli-Opitz syndrome). We will discuss possible mechanisms by which cholesterol content in the plasma membrane influences synaptic processes. Changes in cholesterol metabolism in Alzheimer's disease, Parkinson's disease, and autistic disorders are beyond the scope of this review and will be summarized in our next paper. © 2016 Park-media, Ltd.


Zalyalova Z.A.,Kazan Medical University
Zhurnal Nevrologii i Psihiatrii imeni S.S. Korsakova | Year: 2014

The most frequent causes of disability of patients with neurological diseases are motor disorders in the upper motor neuron lesion caused by the damage of the brain and/or the spinal cord that resulted in the formation of spastic paresis and paralysis. The correct understanding of the pathophysiological basis of clinical presentations of the upper motor neuron lesion will allow to chose the most adequate and prognostically successful methods of treatment. Currently, treatment with botulotoxin can be considered as such a method. This method in the combination with non-pharmacological rehabilitation decreases the activity of phasic and tonic stretching reflexes, associated contractions, synkinesia, spastic dystonia and spasticity that leads to the increase in muscle elasticity, mobility of extremities, reduction of pain, joint stiffness and soft tissue deformation that, in its turn, can increase the independence of the patient from any help. © 2014, Media Sphera. All rights reserved.


Gerasimova E.,Kazan Federal University | Lebedeva J.,Kazan Federal University | Yakovlev A.,Kazan Federal University | Zefirov A.,Kazan Medical University | And 2 more authors.
Neuroscience | Year: 2015

Hydrogen sulfide (H2S) is a widespread gasotransmitter also known as a powerful neuroprotective agent in the central nervous system. However, the action of H2S in peripheral synapses is much less studied. In the current project we studied the modulatory effects of the H2S donor sodium hydrosulfide (NaHS) on synaptic transmission in the mouse neuromuscular junction using microelectrode technique. Using focal recordings of presynaptic response and evoked transmitter release we have shown that NaHS (300μM) increased evoked end-plate currents (EPCs) without changes of presynaptic waveforms which indicated the absence of NaHS effects on sodium and potassium currents of motor nerve endings. Using intracellular recordings it was shown that NaHS increased the frequency of miniature end-plate potentials (MEPPs) without changing their amplitudes indicating a pure presynaptic effect. Furthermore, NaHS increased the amplitude of end-plate potentials (EPPs) without influencing the resting membrane potential of muscle fibers. l-cysteine, a substrate of H2S synthesis induced, similar to NaHS, an increase of EPC amplitudes whereas inhibitors of H2S synthesis (β-cyano-l-alanine and aminooxyacetic acid) had the opposite effect. Inhibition of adenylate cyclase using MDL 12,330A hydrochloride (MDL 12,330A) or elevation of cAMP level with 8-(4-chlorophenylthio)-adenosine 3′,5′-cyclic monophosphate (pCPT-cAMP) completely prevented the facilitatory action of NaHS indicating involvement of the cAMP signaling cascade. The facilitatory effect of NaHS was significantly diminished when intracellular calcium (Ca2+) was buffered by 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis acetoxymethyl ester (BAPTA-AM) and ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid acetoxymethyl ester (EGTA-AM). Activation of ryanodine receptors by caffeine or ryanodine increased acetylcholine release and prevented further action of NaHS on transmitter release, likely due to an occlusion effect. Inhibition of ryanodine receptors by ryanodine or dantrolene also reduced the action of NaHS on EPC amplitudes. Our results indicate that in mammalian neuromuscular synapses endogenously produced H2S increases spontaneously and evoked quantal transmitter release from motor nerve endings without changing the response of nerve endings. The presynaptic effect of H2S appears mediated by intracellular Ca2+ and cAMP signaling and involves presynaptic ryanodine receptors. © 2015 IBRO.


PubMed | Kazan Medical University
Type: | Journal: Khirurgiia | Year: 2016

Post-sternotomy mediastinitis is a severe complication of cardiac surgery and accompanied by high mortality and cost of treatment. To date, there are no large clinical trials defining treatment of these patients. In this study the authors offer early postoperative wound sanitation, use of vacuum drainage in all cases and surgical repair with local tissues. Sternal destruction is cured by osteosynthesis with wire sutures and perforated metal plates. Proposed algorithm showed low incidence of reccurent wound infection, mortality and duration of treatment.


PubMed | Kazan Medical University
Type: Journal Article | Journal: Acta naturae | Year: 2016

Cholesterol is an important constituent of cell membranes and plays a crucial role in the compartmentalization of the plasma membrane and signaling. Brain cholesterol accounts for a large proportion of the bodys total cholesterol, existing in two pools: the plasma membranes of neurons and glial cells and the myelin membranes . Cholesterol has been recently shown to be important for synaptic transmission, and a link between cholesterol metabolism defects and neurodegenerative disorders is now recognized. Many neurodegenerative diseases are characterized by impaired cholesterol turnover in the brain. However, at which stage the cholesterol biosynthetic pathway is perturbed and how this contributes to pathogenesis remains unknown. Cognitive deficits and neurodegeneration may be associated with impaired synaptic transduction. Defects in cholesterol biosynthesis can trigger dysfunction of synaptic transmission. In this review, an overview of cholesterol turnover under physiological and pathological conditions is presented (Huntingtons, Niemann-Pick type C diseases, Smith-Lemli-Opitz syndrome). We will discuss possible mechanisms by which cholesterol content in the plasma membrane influences synaptic processes. Changes in cholesterol metabolism in Alzheimers disease, Parkinsons disease, and autistic disorders are beyond the scope of this review and will be summarized in our next paper.

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